利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

17 条记录 (耗时 0.017 秒)

    Tumor marker protein and antibodies thereto for cancer risk assessment or diagnosis
    4.
    发明授权
    Tumor marker protein and antibodies thereto for cancer risk assessment or diagnosis 失效
    肿瘤标志物蛋白及其抗体用于癌症风险评估或诊断

    公开(公告)号：US5310653A

    公开(公告)日：1994-05-10

    申请号：US12972

    申请日：1993-02-02

    申请人： Margaret Hanausek-Walaszek ,  Thomas J. Slaga ,  Zbigniew Walaszek

    发明人： Margaret Hanausek-Walaszek ,  Thomas J. Slaga ,  Zbigniew Walaszek

    IPC分类号： C07K14/47 ,  C07K16/18 ,  G01N33/574 ,  A61K37/08 ,  A61K39/00 ,  C07K15/18

    CPC分类号： G01N33/57476 ,  C07K14/47 ,  C07K16/18 ,  G01N33/5743 ,  G01N33/57438 ,  G01N33/57496

    摘要： A tumor-associated marker protein was purified and antibodies thereto developed for cancer diagnosis and assessment of cancer risk associated with the long-term use of synthetic steroid hormones, both contraceptive and non-contraceptive, and other drugs that exhibit tumor promotional properties. The marker protein and antibodies thereto provided are interspecies immunologically cross-reactive.In summary, the marker p65 tumor-associated factor of the present invention has the following characteristics:(a) binds substantially completely to a phenyl hydrophobic interaction column in a buffer containing 20% ammonium sulfate and eluted at ca. 16% ammonium sulfate;(b) localized primarily in the nuclear envelopes with only small amounts present in the cytoplasm from where is released to the blood circulation in vivo or cell culture medium in vitro;(c) induced in normal, adult tissues by chemical carcinogens (initiators) but not by tumor promoters, the carcinogen-induced production being enhanced by the latter.Also disclosed herein are processes for purifying the 65 kDa tumor marker from plasma, tumor cytosol or ascitic fluid of carcinoma bearing animals; processes for producing antisera and purified antibody preparations to the 65 kDa tumor marker; and methods using antibody to the 65 kDa to diagnose or assess the likelihood of cancer.

    摘要翻译： 纯化了与肿瘤相关的标记蛋白，并且其抗体开发用于癌症诊断和评估与长期使用合成类固醇激素（避孕和非避孕药）以及其它展现肿瘤促进性质的药物相关的癌症风险。 标记蛋白及其提供的抗体是免疫交叉反应性的种间。 总之，本发明的标记p65肿瘤相关因子具有以下特征：（a）基本上完全与含有20％硫酸铵的缓冲液中的苯基疏水相互作用柱结合并在约5℃下洗脱。 16％硫酸铵; （b）主要在核包膜中定位，其中只有少量存在于细胞质中，其体内释放到体内血液循环或体外细胞培养基; （c）通过化学致癌物（引发剂）而不是由肿瘤启动子在正常的成年组织中诱导，致癌物诱导的生产由后者增强。 本文还公开了从承载动物的血浆，肿瘤细胞质或腹水中纯化65kDa肿瘤标志物的方法; 制备抗血清和纯化抗体制剂至65kDa肿瘤标志物的方法; 以及使用65kDa抗体诊断或评估癌症可能性的方法。

    Processing method for manufacturing black tea and an improved black tea
    6.
    发明授权
    Processing method for manufacturing black tea and an improved black tea 失效
    制造红茶和改良红茶的加工方法

    公开(公告)号：US06811799B2

    公开(公告)日：2004-11-02

    申请号：US10625500

    申请日：2003-07-22

    申请人： Jifu Zhao ,  Thomas J. Slaga

    发明人： Jifu Zhao ,  Thomas J. Slaga

    IPC分类号： A23F300

    CPC分类号： C12Y110/03001 ,  A23F3/10

    摘要： Fresh tea leaves or green tea are converted to black tea using tyrosinase oxidation which alters catechines (a group of green tea polyphenols) to theaflavins. Theaflavins are unique black tea polyphenols and a potential anti-sunburn and chemopreventive agents. The oxidation reaction is performed on leaves which contain moisture only in an amount that the oxidation reaction occurs under the conditions of temperature of 20-50° C., air or O2 (0.2 to 2 moles O2/kg dry tea) supply and pH 5.0 to 7.5 at localized sites on and within the tea leaf structure. The moisture is supplied by moistening fresh tea leaves or dry green tea to a limited degree with a proper amount and concentration of monophenol, catechol and polyphenol oxidases, and tyrosinase, or their combinations. Obtained black tea contains much more theaflavins than regular black tea, and is, therefore, a more healthful beverage than that produced through use of conventionally processed black tea.

    摘要翻译： 使用酪氨酸酶氧化将新鲜的茶叶或绿茶转化为红茶，其将茶几（一组绿茶多酚）改变为茶黄素。 茶黄素是独特的红茶多酚和潜在的抗晒伤和化学预防剂。 在温度为20-50℃，空气或O2（0.2〜2摩尔O 2 / kg干茶）供给条件下的氧化反应发生量和pH5.0的条件下，对含有水分的叶子进行氧化反应 到茶叶结构内部和内部的局部位置为7.5。 通过用适量和浓度的苯酚，儿茶酚和多酚氧化酶和酪氨酸酶或它们的组合，在有限程度上润湿新鲜茶叶或干绿茶来供应水分。 获得的红茶比常规红茶含有更多的茶黄素，因此比通过使用常规加工的红茶产生的饮料更健康。

    Method for promoting extraction of beneficial compounds into beverages naturally containing same, and for extending the duration of freshness of coffee
    7.
    发明授权
    Method for promoting extraction of beneficial compounds into beverages naturally containing same, and for extending the duration of freshness of coffee 有权
    用于促进将有益化合物提取到天然含有它们的饮料中并延长咖啡新鲜度的方法

    公开(公告)号：US06669979B1

    公开(公告)日：2003-12-30

    申请号：US09481279

    申请日：2000-01-11

    申请人： Jifu Zhao ,  Thomas J. Slaga ,  Rajesh Agarwal ,  Brian Slaga

    发明人： Jifu Zhao ,  Thomas J. Slaga ,  Rajesh Agarwal ,  Brian Slaga

    IPC分类号： A23F500

    CPC分类号： A23F5/14 ,  A23F5/267

    摘要： A process for increasing the levels of polyphenolic acid release into coffee which is brewed from beans which are bathed in an aqueous sodium bicarbonate solution shortly after roasting. The same process is applied to other polyphenolics containing beverage substrates, including teas. The bathing of beverage substrates (coffee beans and tea leaves, for example) with the aqueous sodium bicarbonate does not adversely change the taste of the produced beverage, and, in fact, in the case of coffee, such process actually extends the apparent freshness of coffee by inhibiting in the condensation of tannins in the coffee brew, thereby providing, not only the health benefits of the elevated levels of polyphenols, but a means to extend the freshness of the brewed beverage.

    摘要翻译： 一种将多酚酸释放到咖啡中的方法，该咖啡从焙烧后不久浸泡在碳酸氢钠水溶液中的豆制成。 相同的方法应用于含有饮料底物的其它多酚，包括茶。 用碳酸氢钠水溶液洗涤饮料底物（例如咖啡豆和茶叶）不会不利地改变所生产的饮料的味道，事实上，在咖啡的情况下，这种方法实际上延长了 咖啡通过抑制咖啡酿造中的单宁酸的冷凝，从而不仅提供了多酚的高水平的健康益处，而且提供了延长酿造的饮料的新鲜度的手段。

    Processing method for manufacturing black tea and an improved black tea
    10.
    发明授权
    Processing method for manufacturing black tea and an improved black tea 有权
    制造红茶和改良红茶的加工方法

    公开(公告)号：US06602527B1

    公开(公告)日：2003-08-05

    申请号：US09721438

    申请日：2000-11-22

    申请人： Thomas J. Slaga ,  Jifu Zhao

    发明人： Thomas J. Slaga ,  Jifu Zhao

    IPC分类号： A23F300

    CPC分类号： C12Y110/03001 ,  A23F3/10

    摘要： Fresh tea leaves or green tea are converted to black tea using tyrosinase oxidation which alters catechines (a group of green tea polyphenols) to theaflavins. Theaflavins are unique black tea polyphenols and a potential anti-sunburn and chemopreventive agents. The oxidation reaction is performed on leaves which contain moisture only in an amount that the oxidation reaction occurs under the conditions of temperature of 20-50° C., air or O2 (0.2 to 2 moles O2/kg dry tea) supply and pH 5.0 to 7.5 at localized sites on and within the tea leaf structure. The moisture is supplied by moistening fresh tea leaves or dry green tea to a limited degree with a proper amount and concentration of monophenol, catechol and polyphenol oxidases, and tyrosinase, or their combinations. Obtained black tea contains much more theaflavins than regular black tea, and is, therefore, a more healthful beverage than that produced through use of conventionally processed black tea.

    摘要翻译： 使用酪氨酸酶氧化将新鲜的茶叶或绿茶转化为红茶，其将茶几（一组绿茶多酚）改变为茶黄素。 茶黄素是独特的红茶多酚和潜在的抗晒伤和化学预防剂。 在温度为20-50℃，空气或O2（0.2〜2摩尔O 2 / kg干茶）供给条件下的氧化反应发生量和pH5.0的条件下，对含有水分的叶子进行氧化反应 到茶叶结构内部和内部的局部位置为7.5。 通过用适量和浓度的苯酚，儿茶酚和多酚氧化酶和酪氨酸酶或它们的组合，在有限程度上润湿新鲜茶叶或干绿茶来供应水分。 获得的红茶比常规红茶含有更多的茶黄素，因此比通过使用常规加工的红茶产生的饮料更健康。