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19 条记录 (耗时 0.027 秒)

    Directed content presentation
    1.
    发明授权
    Directed content presentation 有权
    定向内容演示

    公开(公告)号：US09451308B1

    公开(公告)日：2016-09-20

    申请号：US13556117

    申请日：2012-07-23

    申请人： Zhenyu Liu ,  Yong Yao

    发明人： Zhenyu Liu ,  Yong Yao

    IPC分类号： H04H60/32 ,  H04N21/262

    CPC分类号： H04N21/26241 ,  H04N21/4126 ,  H04N21/4394 ,  H04N21/44227 ,  H04N21/812

    摘要： Methods, systems, and apparatus, including computer programs encoded on computer storage media, for content presentation. One of the methods includes determining current programming being presented to a user from a first device, wherein the determining includes receiving information from the first device associated with the current programming being presented; determining one or more sponsored content items to provide to a second device, wherein the one or more sponsored content items are identified as associated with the current programming including determining that one or more presentation criteria as specified by a respective sponsored content provider have been satisfied; and providing the one or more sponsored content items to the second device.

    摘要翻译： 方法，系统和装置，包括在计算机存储介质上编码的计算机程序，用于内容呈现。 所述方法之一包括确定从第一设备呈现给用户的当前节目，其中所述确定包括从所述第一设备接收与正在呈现的当前节目相关联的信息; 确定一个或多个赞助的内容项目以提供给第二设备，其中所述一个或多个赞助的内容项目被识别为与当前节目相关联，包括确定已经满足由相应的赞助内容提供商指定的一个或多个呈现准则; 以及将所述一个或多个赞助的内容项目提供给所述第二设备。

    Cell-based assay for G-protein-coupled receptor-mediated activity employing a mutated cyclic nucleotide-gated ion channel and a membrane potential dye
    2.
    发明授权
    Cell-based assay for G-protein-coupled receptor-mediated activity employing a mutated cyclic nucleotide-gated ion channel and a membrane potential dye 有权
    使用突变的环核苷酸门控离子通道和膜电位染料的G蛋白偶联受体介导活性的基于细胞的测定

    公开(公告)号：US07384755B2

    公开(公告)日：2008-06-10

    申请号：US11492216

    申请日：2006-07-25

    申请人： Yong Yao ,  Liang Cao

    发明人： Yong Yao ,  Liang Cao

    IPC分类号： G01N33/53 ,  G01N33/566

    CPC分类号： G01N33/566 ,  G01N2333/726 ,  G01N2500/02 ,  G01N2500/04 ,  G01N2500/10

    摘要： Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.

    摘要翻译： 公开了其用途的组合物和方法，例如鉴定G-蛋白偶联受体，调节G蛋白偶联受体活性的配体和化合物。 组合物和方法使用环状核苷酸门控通道和荧光染料来检测响应于G蛋白偶联受体的刺激的细胞内cAMP水平的变化。 可以在多种测定中检测G蛋白偶联受体的激活，包括使用荧光显微镜的基于细胞的成像测定和具有多孔板和荧光板读数器的高通量测定。

    Gαq protein variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors
    4.
    发明授权
    Gαq protein variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors 有权
    Galphaq蛋白质变体及其在分析和发现化学感应受体激动剂和拮抗剂中的用途

    公开(公告)号：US07041457B2

    公开(公告)日：2006-05-09

    申请号：US09989497

    申请日：2001-11-21

    申请人： Yong Yao ,  Hong Xu

    发明人： Yong Yao ,  Hong Xu

    IPC分类号： G01N33/53

    CPC分类号： C07K14/4722 ,  C07K2319/00

    摘要： The invention provides a series of Gαq protein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in intracellular IP3, or calcium. In a particular embodiment, the Gαq protein variants can be expressed in mammalian cell lines or Xenopus oocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording.

    摘要翻译： 本发明提供了一系列以过度混杂的方式功能性地与感觉细胞受体例如味道GPCR（TR））和嗅觉GPCR（OR）偶联的Galpha蛋白质变体。 根据本发明，功能性偶联可以通过例如测量细胞内IP3或钙的变化来确定。 在一个具体实施方案中，GalphaQ蛋白变体可以在哺乳动物细胞系或非洲爪蟾卵母细胞中表达，然后使用钙荧光成像和电生理记录进行评估。

    G&agr;q protein variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors
    5.
    发明授权
    G&agr;q protein variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors 有权
    Galphaq蛋白质变体及其在分析和发现化学感应受体激动剂和拮抗剂中的用途

    公开(公告)号：US06818747B2

    公开(公告)日：2004-11-16

    申请号：US09984292

    申请日：2001-10-29

    申请人： Yong Yao ,  Hong Xu

    发明人： Yong Yao ,  Hong Xu

    IPC分类号： C07K1900

    CPC分类号： C07K14/4722 ,  C07K2319/00

    摘要： The invention provides a series of G&agr;q protein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in intracellular IP3, or calcium. In a particular embodiment, the G&agr;q protein variants can be expressed in mammalian cell lines or Xenopus oocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording.

    摘要翻译： 本发明提供了一系列Galphaq蛋白质变体，其以过度混杂的方式功能性地耦合到感觉细胞受体，例如味道GPCR（TR）和嗅觉GPCR（OR））。 根据本发明，功能性偶联可以通过例如测量细胞内IP3或钙的变化来确定。 在一个具体实施方案中，Galphaq蛋白质变体可以在哺乳动物细胞系或非洲爪蟾卵母细胞中表达，然后使用钙荧光成像和电生理记录进行评估。

    Gαq protein variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors
    6.
    发明授权
    Gαq protein variants and their use in the analysis and discovery of agonists and antagonists of chemosensory receptors 有权
    Galphaq蛋白质变体及其在分析和发现化学感应受体激动剂和拮抗剂中的用途

    公开(公告)号：US07291485B2

    公开(公告)日：2007-11-06

    申请号：US10952773

    申请日：2004-09-30

    申请人： Yong Yao ,  Hong Xu

    发明人： Yong Yao ,  Hong Xu

    IPC分类号： C12N15/62

    CPC分类号： C07K14/4722 ,  C07K2319/00

    摘要： The invention provides a series of Gαq protein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in intracellular IP3, or calcium. In a particular embodiment, the Gαq protein variants can be expressed in mammalian cell lines or Xenopus oocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording.

    摘要翻译： 本发明提供了一系列以过度混杂的方式功能性地与感觉细胞受体例如味道GPCR（TR））和嗅觉GPCR（OR）偶联的Galpha蛋白质变体。 根据本发明，功能性偶联可以通过例如测量细胞内IP3或钙的变化来确定。 在一个具体实施方案中，GalphaQ蛋白变体可以在哺乳动物细胞系或非洲爪蟾卵母细胞中表达，然后使用钙荧光成像和电生理记录进行评估。

    Novel cell-based assays for G-protein-coupled receptor-mediated activities
    7.
    发明申请
    Novel cell-based assays for G-protein-coupled receptor-mediated activities 有权
    用于G蛋白偶联受体介导的活性的新型基于细胞的测定

    公开(公告)号：US20070178483A1

    公开(公告)日：2007-08-02

    申请号：US11492216

    申请日：2006-07-25

    申请人： Yong Yao ,  Liang Cao

    发明人： Yong Yao ,  Liang Cao

    IPC分类号： C12Q1/68 ,  G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705 ,  C12N5/06 ,  C12N5/08

    CPC分类号： G01N33/566 ,  G01N2333/726 ,  G01N2500/02 ,  G01N2500/04 ,  G01N2500/10

    摘要： Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.

    摘要翻译： 公开了其用途的组合物和方法，例如鉴定G-蛋白偶联受体，调节G蛋白偶联受体活性的配体和化合物。 组合物和方法使用环状核苷酸门控通道和荧光染料来检测响应于G蛋白偶联受体的刺激的细胞内cAMP水平的变化。 可以在多种测定中检测G蛋白偶联受体的激活，包括使用荧光显微镜的基于细胞的成像测定和具有多孔板和荧光板读数器的高通量测定。

    Cell-based assays employing voltage and calcium dyes
    8.
    发明授权
    Cell-based assays employing voltage and calcium dyes 有权
    使用电压和钙染料的基于细胞的测定

    公开(公告)号：US07238213B2

    公开(公告)日：2007-07-03

    申请号：US10978282

    申请日：2004-11-01

    申请人： Yong Yao ,  Liang Cao ,  Jianming Lu ,  Isabel Llorente

    发明人： Yong Yao ,  Liang Cao ,  Jianming Lu ,  Isabel Llorente

    IPC分类号： C09B51/00 ,  A61B5/0275 ,  G01N33/567

    CPC分类号： C07K14/705 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/02 ,  G01N2500/04 ,  G01N2500/10

    摘要： Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.

    摘要翻译： 公开了使用环状核苷酸门控通道和荧光染料以及其他指示剂来检测细胞内cAMP水平的变化的新型组合物和方法，例如响应于G蛋白偶联受体的刺激。 包括一种或多种增加对cAMP的敏感性和减少二价阳离子介导的阻断的孔突变的CNG突变体用于多种测定，包括G蛋白偶联受体活性的检测。 还公开了特别适用于蛋白质相互作用的基于细胞的测定的新型染料猝灭剂制剂。