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    Methods for identifying and monitoring drug side effects
    3.
    发明授权
    Methods for identifying and monitoring drug side effects 有权
    鉴别和监测药物副作用的方法

    公开(公告)号：US07883858B2

    公开(公告)日：2011-02-08

    申请号：US12468834

    申请日：2009-05-19

    申请人： Leroy Hood ,  Biaoyang Lin

    发明人： Leroy Hood ,  Biaoyang Lin

    IPC分类号： G01N33/53

    CPC分类号： G01N33/57434 ,  G01N33/6842 ,  G01N33/6893

    摘要： The present invention relates generally to methods for identifying drug side effects by detecting perturbations in organ-specific molecular blood fingerprints. The invention further relates to methods for identifying drug-specific organ-specific molecular blood fingerprints. As such, the present invention provides compositions comprising organ-specific proteins, detection reagents for detecting such proteins, and panels and arrays for determining organ-specific molecular blood fingerprints.

    摘要翻译： 本发明一般涉及通过检测器官特异性分子血液指纹图中的扰动来鉴定药物副作用的方法。 本发明还涉及用于鉴定药物特异性器官特异性分子血液指纹图谱的方法。 因此，本发明提供了包含器官特异性蛋白质的组合物，用于检测这些蛋白质的检测试剂，以及用于测定器官特异性分子血液指纹图谱和阵列。

    THERMOSTABLE POLYMERASES HAVING ALTERED FIDELITY AND METHODS OF IDENTIFYING AND USING SAME
    4.
    发明申请
    THERMOSTABLE POLYMERASES HAVING ALTERED FIDELITY AND METHODS OF IDENTIFYING AND USING SAME 审中-公开
    具有变化的可塑性聚合物及其识别方法和使用方法

    公开(公告)号：US20090004656A1

    公开(公告)日：2009-01-01

    申请号：US11962067

    申请日：2007-12-20

    申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    IPC分类号： C12Q1/68 ,  C12Q1/48 ,  C12N9/10 ,  C12N15/11 ,  C12P19/34

    CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

    摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

    摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。

    Thermostable polymerases having altered fidelity and method of identifying and using same
    10.
    发明授权
    Thermostable polymerases having altered fidelity and method of identifying and using same 失效
    具有改变的保真度的热稳定性聚合酶和鉴定和使用它们的方法

    公开(公告)号：US06395524B2

    公开(公告)日：2002-05-28

    申请号：US08978806

    申请日：1997-11-26

    申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    IPC分类号： C12N910

    CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

    摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

    摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。