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    Multiplexed method for the identification and quantitation of minor alleles and polymorphisms
    1.
    发明授权
    Multiplexed method for the identification and quantitation of minor alleles and polymorphisms 有权

    公开(公告)号：US11680289B2

    公开(公告)日：2023-06-20

    申请号：US17098993

    申请日：2020-11-16

    申请人： Agena Bioscience, Inc.

    发明人： Anders Olof Herman Nygren

    IPC分类号： C12P19/34 ,  C12Q1/6858 ,  C12Q1/6827 ,  C12Q1/686

    CPC分类号： C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6827 ,  C12Q1/6827 ,  C12Q2525/186 ,  C12Q2527/137 ,  C12Q2535/125 ,  C12Q2537/143 ,  C12Q2545/101

    摘要： Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

    Preparation enhancements and methods of use for MALDI mass spectrometry
    4.
    发明授权
    Preparation enhancements and methods of use for MALDI mass spectrometry 有权
    MALDI质谱法的制备增强和使用方法

    公开(公告)号：US09305756B2

    公开(公告)日：2016-04-05

    申请号：US13801526

    申请日：2013-03-13

    申请人： AGENA BIOSCIENCE, INC

    发明人： Thomas Becker ,  Stefan Berkenkamp

    IPC分类号： G01N24/00 ,  H01J49/04 ,  H01J49/16

    CPC分类号： H01J49/0418 ,  G01N33/483 ,  H01J49/164

    摘要： Provided herein are compositions and methods useful for preparing and analyzing a sample on a substrate by matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS). In some embodiments, compositions provided herein comprise a substrate, matrix and nanoparticles, and sometimes comprise one or more additives and sometimes an analyte. Compositions provided herein sometimes comprise nanoparticles that include or are made up of silicon dioxide.

    摘要翻译： 本文提供了可用于通过基质辅助激光解吸电离（MALDI）质谱法（MS）在基底上制备和分析样品的组合物和方法。 在一些实施方案中，本文提供的组合物包含底物，基质和纳米颗粒，并且有时包含一种或多种添加剂，有时包含分析物。 本文提供的组合物有时包括包含或由二氧化硅组成的纳米颗粒。

    MULTIPLEXED METHOD FOR THE IDENTIFICATION AND QUANTITATION OF MINOR ALLELES AND POLYMORPHISMS
    6.
    发明申请
    MULTIPLEXED METHOD FOR THE IDENTIFICATION AND QUANTITATION OF MINOR ALLELES AND POLYMORPHISMS 有权

    公开(公告)号：US20210062254A1

    公开(公告)日：2021-03-04

    申请号：US17098993

    申请日：2020-11-16

    申请人： Agena Bioscience, Inc.

    发明人： Anders Olof Herman NYGREN

    IPC分类号： C12Q1/6858 ,  C12Q1/6827 ,  C12Q1/686

    摘要： Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

    Compositions and processes for improved mass spectrometry analysis
    7.
    发明授权
    Compositions and processes for improved mass spectrometry analysis 有权

    公开(公告)号：US10329612B2

    公开(公告)日：2019-06-25

    申请号：US15840432

    申请日：2017-12-13

    申请人： AGENA BIOSCIENCE, INC.

    发明人： Thomas Becker

    IPC分类号： C12Q1/6872 ,  G01N33/68

    摘要： The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.

    Base specific cleavage of methylation-specific amplification products in combination with mass analysis
    8.
    发明授权
    Base specific cleavage of methylation-specific amplification products in combination with mass analysis 有权
    甲基化特异性扩增产物的碱基特异性切割与质量分析相结合

    公开(公告)号：US09249456B2

    公开(公告)日：2016-02-02

    申请号：US11089805

    申请日：2005-03-24

    申请人： Mathias Ehrich ,  Dirk Johannes Van Den Boom

    发明人： Mathias Ehrich ,  Dirk Johannes Van Den Boom

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6848 ,  C12Q1/6858 ,  C12Q2565/627 ,  C12Q2523/125 ,  C12Q2525/143

    摘要： Methods, combinations and kits are provided for identifying the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or for identifying the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule. Methylation state identification is performed by treating a methylated target nucleic acid molecule with a reagent that modifies one or more nucleotides in the target nucleic acid molecule as a function of the methylation state of the target nucleic acid molecule, methylation specifically amplifying treated target nucleic acid molecule, fragmenting amplified products, and detecting one or more fragments to thereby identify the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule.

    摘要翻译： 提供了用于鉴定靶核酸分子的甲基化状态，靶核酸分子中的核苷酸位点的甲基化状态或鉴定靶核酸中一个或多个甲基化或非甲基化核苷酸的位点的方法，组合和试剂盒 酸分子。 通过用靶核酸分子修饰一个或多个核苷酸的试剂处理甲基化靶核酸分子，作为靶核酸分子的甲基化状态的函数，特异性扩增处理的靶核酸分子的甲基化，进行甲基化状态鉴定 ，片段扩增产物，检测一个或多个片段，从而鉴定靶核酸分子的甲基化状态，靶核酸分子中的核苷酸位点的甲基化状态或一个或多个甲基化或非甲基化核苷酸的位点 靶核酸分子。