公开(公告)号：US20220013343A1

公开(公告)日：2022-01-13

申请号：US17186650

申请日：2021-02-26

申请人： Bruker Daltonik GmbH

发明人： Tobias BOSKAMP

IPC分类号： H01J49/00 ,  G01N33/483

摘要： The disclosure relates to a method for processing a mass spectrum, comprising: (i) providing the mass spectrum which contains a plurality of data pairs, each data pair being representative of a mass value or mass-related value on a mass scale or mass-related scale and an abundance value or abundance-related value associated with the respective mass value or mass-related value, (ii) selecting a plurality of intervals on the mass scale or mass-related scale, each interval containing a multitude of the said data pairs, (iii) for each interval, applying a first mathematical-statistical analysis to the said data pairs contained in the respective interval in order to derive an interval-specific peak width, and (iv) using the said interval-specific peak widths to determine an estimated peak width for each mass value or mass-related value on the mass scale or mass-related scale.

公开(公告)号：US20210217601A1

公开(公告)日：2021-07-15

申请号：US17219230

申请日：2021-03-31

申请人： Bruker Daltonik GmbH

发明人： Martin SCHÜRENBERG ,  Alexander VOSSGRÖNE

IPC分类号： H01J49/04 ,  G01N1/28 ,  B01L3/00

摘要： The invention relates to methods for drawing-off liquid from individual droplets which are in a predefined arrangement on a flat substrate and have sedimented material enclosed in them. A mask of an absorbent material comprising a pattern of indentations or holes which corresponds at least partially to the regular arrangement of the individual droplets, or a stiff, rigid plate of an absorbent material is positioned above the flat substrate in such a way that the droplets come into contact with the absorbent material peripherally so that liquid is drawn off there-into. The invention also relates to a mask of an absorbent material with a substantially rectangular shape which has a predefined pattern of indentations or holes for the purpose of separating liquid and sedimented material enclosed therein.

公开(公告)号：US11049705B2

公开(公告)日：2021-06-29

申请号：US16359491

申请日：2019-03-20

申请人： Bruker Daltonik GmbH

发明人： Sebastian Böhm ,  Andreas Haase ,  Jens Höhndorf

IPC分类号： H01J49/02 ,  H01J49/40 ,  H01J49/00

摘要： The disclosure relates to a method of operating a secondary-electron multiplier in the ion detector of a mass spectrometer so as to prolong the service life, wherein the secondary-electron multiplier is supplied with an operating voltage in such a way that an amplification of less than 106 secondary electrons per impinging ion results, while the output current of the secondary-electron multiplier is amplified using an electronic preamplifier mounted close to the secondary-electron multiplier with such a low noise level that the current pulses of individual ions impinging on the ion detector are detected above the noise at the input of a digitizing unit. Further disclosed are the use of the methods for imaging mass spectrometric analysis of a thin tissue section or mass spectrometric high-throughput analysis/massive-parallel analysis, and a time-of-flight mass spectrometer whose control unit is programmed to execute such methods.

公开(公告)号：US11017993B2

公开(公告)日：2021-05-25

申请号：US15912694

申请日：2018-03-06

申请人： Bruker Daltonik GmbH

发明人： Martin Schürenberg ,  Alexander Vossgröne

IPC分类号： H01J49/04 ,  B01L3/00 ,  G01N1/28

摘要： The invention relates to methods for drawing-off liquid from individual droplets which are in a predefined arrangement on a flat substrate and have sedimented material enclosed in them. A mask of an absorbent material comprising a pattern of indentations or holes which corresponds at least partially to the regular arrangement of the individual droplets, or a stiff, rigid plate of an absorbent material is positioned above the flat substrate in such a way that the droplets come into contact with the absorbent material peripherally so that liquid is drawn off there-into. The invention also relates to a mask of an absorbent material with a substantially rectangular shape which has a predefined pattern of indentations or holes for the purpose of separating liquid and sedimented material enclosed therein.

公开(公告)号：US10937639B2

公开(公告)日：2021-03-02

申请号：US16429576

申请日：2019-06-03

申请人： Bruker Daltonik GmbH

发明人： Jens Decker

IPC分类号： H01J49/00 ,  H01J49/40

摘要： The invention relates to selection of precursors from a measured mobility-mass map for tandem mass spectrometry and is based on processing a peak list from measured signals and clustering these peaks in the mobility-mass space.

公开(公告)号：US08941054B2

公开(公告)日：2015-01-27

申请号：US13094128

申请日：2011-04-26

申请人： Melvin Andrew Park ,  Desmond Allen Kaplan ,  Mark Ridgeway

发明人： Melvin Andrew Park ,  Desmond Allen Kaplan ,  Mark Ridgeway

IPC分类号： H01J49/00 ,  G01N27/62

CPC分类号： H01J49/06 ,  G01N27/622 ,  G01N27/624

摘要： Ions with a predetermined range of ion mobilities are produced by filtering input ions with at least two consecutive ion mobility high pass and/or low pass filters. Each ion mobility filter is formed by entraining ions in a moving gas and applying a DC electric field to the ions which causes the ions to move in a direction opposite to the gas flow. An ion mobility high pass filter is formed when the DC electric field drives the ions against the flow of gas, whereas an ion mobility low pass filter is formed when a the gas flow drives entrained ions against an DC electric field barrier.

摘要翻译： 具有预定范围的离子迁移率的离子通过用至少两个连续的离子迁移率高通和/或低通滤波器过滤输入离子而产生。 每个离子迁移率过滤器是通过夹杂在移动气体中的离子形成的，并且对离子施加直流电场，这导致离子沿与气流相反的方向移动。 当DC电场驱动离子抵抗气体流时，形成离子迁移率高通滤波器，而当气流驱动夹带的离子抵抗DC电场屏障时，形成离子迁移率低通滤波器。

公开(公告)号：US08927928B2

公开(公告)日：2015-01-06

申请号：US13465669

申请日：2012-05-07

申请人： Petra Decker ,  Oliver Räther

发明人： Petra Decker ,  Oliver Räther

IPC分类号： H01J49/40 ,  H01J49/00

CPC分类号： H01J49/0031 ,  H01J49/401

摘要： Methods are provided for acquiring sum spectra in a time-of-flight mass spectrometer with orthogonal pulsed acceleration, where each of the sum spectra is obtained from a plurality of summed individual spectra. The mass spectrometer has an ion storage device that collects the ions temporarily before they are transferred to an ion pulser, which pulses out the ions orthogonally. Acquisition conditions such as, for example, delay times between opening the ion storage device and the pulsed ejection in the ion pulser are varied for the individual spectra, which are added together to form the sum spectrum of ions with light masses and high masses.

摘要翻译： 提供了用于在具有正交脉冲加速度的飞行时间质谱仪中获取总和光谱的方法，其中每个和光谱从多个相加的单个光谱获得。 质谱仪具有离子存储装置，其在离子脉冲发生器转移到离子脉冲发生器之前临时收集离子，从而正交地离子出离子。 对于各个光谱而言，采集条件例如打开离子存储装置之间的延迟时间和在离子脉冲发生器中的脉冲喷射之间是变化的，这些光谱被加在一起以形成具有光质量和高质量的离子的和光谱。

公开(公告)号：US08912485B2

公开(公告)日：2014-12-16

申请号：US13103672

申请日：2011-05-09

申请人： Thomas Maier ,  Jochen Franzen

发明人： Thomas Maier ,  Jochen Franzen

IPC分类号： H01J49/00 ,  H01J49/16 ,  H01J49/40

CPC分类号： H01J49/0027 ,  H01J49/164 ,  H01J49/40

摘要： The invention relates to acquisition techniques for time-of-flight mass spectra with ionization of the analyte substances by matrix assisted laser desorption. Generally speaking, these acquisition techniques involve adding together a large number of individual time-of-flight spectra, each with restricted dynamic measuring range, to form a sum spectrum. The invention provides a method that improves, in particular, the reproducibility, the concentration accuracy and therefore the ability to quantify the mass spectra. Particular embodiments also increase the dynamic range of measurement. For this purpose, multiple series of mass spectra are acquired, whereby the energy density in the laser spot is increased in discrete steps. As a result, many ion signals saturate the detector and can therefore no longer be evaluated. However, it is possible to employ a technique in which the ion beam is increasingly defocused, or, secondly, to replace parts of the spectrum that are subject to saturation by intensity extrapolations from mass spectra acquired with lower energy density. In the first case, hundreds or thousands of individual mass spectra must be added together in order to increase the dynamic measuring range. In the second case, the finally acquired mass spectrum, with its replacements, forms a mass spectrum with a high dynamic measuring range, improved reproducibility and better concentration accuracy. The gradient of the increasing intensities of the ion signals, as a function of the energy density, supplies additional information about the proton affinity of the analyte ions. The concentration accuracy is enhanced because the increase in the number of proton donors in the ionization plasma leads to an increase in the ionization of those analyte substances that have a lower proton affinity.

摘要翻译： 本发明涉及通过基质辅助激光解吸附物质分析物质离子化的飞行时间质谱采集技术。 一般来说，这些采集技术包括将大量具有限制动态测量范围的单个飞行时间频谱相加在一起以形成和频谱。 本发明提供了一种特别提高重现性，浓度精度以及因此量化质谱的能力的方法。 具体实施例还增加了测量的动态范围。 为此，获得了多个质谱系列，从而在离散步骤中激光斑点的能量密度增加。 因此，许多离子信号使检测器饱和，因此不再能被评估。 然而，可以采用这样一种技术，其中离子束越来越散焦，或者其次，通过由具有较低能量密度获得的质谱的强度外推替代经受饱和的部分光谱。 在第一种情况下，为了增加动态测量范围，必须将数百或数千个单个质谱加在一起。 在第二种情况下，最终获得的质谱与其替代物形成具有高动态测量范围，改进的再现性和更好的浓度精度的质谱。 作为能量密度的函数的离子信号的增加强度的梯度提供关于分析物离子的质子亲和力的附加信息。 由于离子化等离子体中的质子供体的数量的增加导致具有较低质子亲和力的分析物质的电离增加，所以浓度精度得到提高。

公开(公告)号：US08907271B2

公开(公告)日：2014-12-09

申请号：US13559006

申请日：2012-07-26

申请人： Claus Köster

发明人： Claus Köster

IPC分类号： H01J49/26 ,  H01J49/42

CPC分类号： H01J49/4245 ,  G01P3/48 ,  G05B9/03 ,  H01J49/26 ,  H01J49/42

摘要： Ions are introduced into a Kingdon ion trap in which the ions can oscillate harmonically in a potential well in the longitudinal direction, essentially decoupled from their transverse motion by a Kingdon ion guide, which can consist of a drill-hole through the wall of the ion trap housing electrodes and a central wire. An injection potential is first applied to the wire, but once the heaviest ions of interest have been injected into the trap, the potential of the wire is switched to the potential of the housing electrodes, to trap the ions in the trap. The ions introduced into the Kingdon ion trap may come from a small ion cloud, located in a Paul trap.

摘要翻译： 离子被引入到Kingdon离子阱中，其中离子可以在纵向方向上在势阱中谐波地振荡，基本上通过Kingdon离子导向器与它们的横向运动分离，Kingdon离子引导件可以由穿过离子壁的钻孔组成 陷阱壳电极和中心线。 首先将注入电位施加到导线上，但是一旦将最重的感兴趣的离子注入陷阱中，则将电线的电位切换到壳体电极的电位，以将离子捕获到陷阱中。 引入金登离子阱的离子可能来自位于保护陷阱中的小离子云。

公开(公告)号：US08877459B2

公开(公告)日：2014-11-04

申请号：US13786498

申请日：2013-03-06

申请人： Bruker Daltonik GmbH

发明人： Ulrich Weller

IPC分类号： C12Q1/04 ,  C12N1/00 ,  G01N33/569 ,  G01N33/68

CPC分类号： C12Q1/04 ,  G01N33/569 ,  G01N33/6851

摘要： Identification of infectious pathogens, particularly viruses, bacteria and other microorganisms is effected with a method whereby pathogens of acute infections can be identified, without first culturing them in external nutrient media, by mass spectrometric measurement of their protein profiles obtained from pathogens directly precipitated from body fluid into pellets by centrifuging. With this method, pathogens which cause acute infections can be identified in less than one hour.

摘要翻译： 感染性病原体，特别是病毒，细菌和其他微生物的鉴定是通过以下方法进行的：通过质谱法测定从身体直接沉淀的病原体获得的蛋白质图谱，可以鉴定出急性感染病原体，而无需先将其培养在外部营养培养基中 通过离心将液体转化成颗粒。 通过这种方法，可以在不到一小时的时间内鉴定引起急性感染的病原体。