公开(公告)号：US07753955B2

公开(公告)日：2010-07-13

申请号：US10580559

申请日：2004-12-10

Applicant: Hank C. K. Wuh

Inventor： Hank C. K. Wuh

IPC: A61F2/10 ,  A61F2/52

CPC classification number: A61L27/38 ,  A61L27/303 ,  A61L27/60 ,  C12N5/0068 ,  C12N5/0075 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/54

Abstract: This present invention describes methods and compositions useful for the reconstruction of various soft tissue features such as lips, areola, and many other features by taking a mold of the skin feature to be replaced, such as the areola, prior to surgical resection, re-creating the size and shape of the soft tissue feature, for example, the nipple and areola, and making a polymer or biopolymer scaffold that is biocompatible, has the ability to allow the epithelization of the skin cells over the polymer, the capability of cell integration into the body of the scaffold, as well as the capability of infiltration of surrounding nerve fibers into the substance of the scaffold, so that the patient may have the benefit of a reconstructed soft tissue feature that not only has the same size and shape and appearance as the native tissue, but also has functional sensation.

Abstract translation: 本发明描述了用于重建各种软组织特征如嘴唇，乳晕和许多其它特征的方法和组合物，其通过在外科手术切除术之前通过取代待替换的皮肤特征的模具，​​例如乳晕， 产生软组织特征的尺寸和形状，例如乳头和乳晕，并且制备生物相容性的聚合物或生物聚合物支架，具有允许皮肤细胞在聚合物上上皮化的能力，细胞整合的能力 进入支架的体内，以及周围神经纤维渗入支架物质的能力，使得患者可能具有不仅具有相同尺寸和形状和外观的重建的软组织特征的益处 作为天然组织，也具有功能感。

公开(公告)号：US20070092550A1

公开(公告)日：2007-04-26

申请号：US10575246

申请日：2004-10-07

Applicant: Ge Ming Lui

Inventor： Ge Ming Lui

IPC: A61K35/32 ,  A61F2/02

CPC classification number: A61L27/3839 ,  A61F2/142 ,  A61K35/12 ,  A61K38/1808 ,  A61K38/1825 ,  A61L27/303 ,  A61L27/34 ,  A61L27/3808 ,  C12N5/0621 ,  C12N2501/115 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/70 ,  C12N2533/90 ,  C08L89/00

Abstract: This invention discloses methods to attach and grow a monolayer of cultured human corneal endothelial cells onto the endothelial side of the stroma synthesized from biopolymer to generate a more bio-equivalent artificial cornea. The approaches will include the use of attachment and growth promoting agents such as fibronectin, laminin, RGDS, collagen type IV, bFGF conjugated with polycarbophil, and EGF conjugated with polycarbophil. The patent also describes a method to create a self-sustaining polymer containing adhesive molecules and growth factors to support the attachment and proliferation of cultured human corneal endothelial cells for corneal transplantation either as a half-thickness device or full-thickness button replacement. An approach for the implantation of cultured retinal pigment epithelial (RPE) cells into the sub-retinal space for treatment of age-related macular degeneration (ARMD) is disclosed in this invention. This method will enable the delivery of the transplanted RPE in a sheet of monolayer cells and will be better suited to perform their physiological function.

Abstract translation: 本发明公开了将培养的人角膜内皮细胞的单层附着和生长到由生物聚合物合成的基质的内皮侧上以产生更生物等效的人造角膜的方法。 这些方法将包括使用附着和生长促进剂如纤连蛋白，层粘连蛋白，RGDS，IV型胶原，与聚卡波非缀合的bFGF和与聚卡波非缀合的EGF。 该专利还描述了一种产生含有粘附分子和生长因子的自维持聚合物的方法，以支持用于角膜移植的培养的人角膜内皮细胞的附着和增殖，作为半厚度装置或全厚度按钮替换。 在本发明中公开了将培养的视网膜色素上皮（RPE）细胞植入用于治疗年龄相关性黄斑变性（ARMD）的亚视网膜空间的方法。 这种方法将能够将移植的RPE递送到单层细胞片中，并且将更适合于执行其生理功能。

公开(公告)号：US09458419B2

公开(公告)日：2016-10-04

申请号：US11680050

申请日：2007-02-28

Applicant: Garry Edgington ,  Shaosheng Dong ,  Ge Ming Lui ,  Hank Wuh ,  Stephen L. Sherman

Inventor： Garry Edgington ,  Shaosheng Dong ,  Ge Ming Lui ,  Hank Wuh ,  Stephen L. Sherman

IPC: A61L2/00 ,  A61L2/18 ,  C11D17/04 ,  C11D3/22 ,  C11D3/37 ,  C11D11/00

CPC classification number: C11D17/041 ,  C11D3/222 ,  C11D3/223 ,  C11D3/225 ,  C11D3/226 ,  C11D3/227 ,  C11D3/228 ,  C11D3/3753 ,  C11D3/3765 ,  C11D3/3773 ,  C11D3/3776 ,  C11D11/0005 ,  C11D11/0023 ,  Y10T428/31855

Abstract: This invention relates to an aqueous polymer composition and to films formed from this aqueous composition. The aqueous composition may be used in a method for removing contaminate material from a substrate. The aqueous polymer composition may comprise: water; and at least one water-soluble film forming polymer. In one embodiment, the aqueous composition may further comprise at least one chelating agent and/or at least one surfactant. The aqueous polymer composition may be applied to a contaminated substrate or to a clean substrate which is subjected to subsequent contamination. The aqueous composition may be dehydrated and/or the polymer may be crosslinked to form a film. When applied to a contaminated substrate, the film may combine with the contaminates. When applied to a clean substrate, the contaminate material may subsequently contact and adhere to the film. The film combined with the contaminate material may be separated from the substrate, with the result being removal of the contaminate material from the substrate.

Abstract translation: 本发明涉及一种水性聚合物组合物和由该水性组合物形成的薄膜。 水性组合物可以用于从基材中除去污染物质的方法。 水性聚合物组合物可以包含：水; 和至少一种水溶性成膜聚合物。 在一个实施方案中，水性组合物还可包含至少一种螯合剂和/或至少一种表面活性剂。 水性聚合物组合物可以施加到受污染的基底或经受后续污染的清洁基底。 水性组合物可以脱水和/或聚合物可以交联以形成膜。 当应用于污染的基材时，该膜可能与污染物结合。 当施加到干净的基底上时，污染物质可以随后接触并粘附到膜上。 与污染物质结合的膜可以与基底分离，结果是从基底去除污染物质。

公开(公告)号：US07931938B2

公开(公告)日：2011-04-26

申请号：US10575247

申请日：2004-10-08

Applicant: Ge Ming Lui

Inventor： Ge Ming Lui

IPC: C23C16/26

CPC classification number: A61L27/3878 ,  A61L27/303 ,  A61L27/383 ,  C12M25/14 ,  C12N5/0068 ,  C12N5/0621 ,  C12N2502/28 ,  C12N2533/10 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/54

Abstract: This present invention describes a method of coating a polymer surface with diamond-like carbon (DLC) to render it useful as a carrier for cells derived from neural crest origin, preferable neuronal cells that form dendrites. The biopolymer to be coated with the DLC will include biodegradable polymers and other implantable biopolymers to act as a carrier system for cell transplantation into the various parts of the body, including the brain, the eye, the central and peripheral nervous system, the lung, the liver, the spleen, the kidney, and the bone and cartilage. The biopolymer can be in sheet form or microparticle form, and can be imbedded with, or incorporated into during its synthesis, attachment or growth promoting reagents to enhance and support neuraonal call attachment and growth. This coating method can also augment other coating agents such as extracellular matrix (ECM) secreted by cultured bovine corneal endothelial cells, as well as adhesive molecules such as fibronectin, laminin, and RGDS. The coating step can be a sequential process where the DLC layer will be added on top of an ECM coated surface or an attachment factor coated surface.

Abstract translation: 本发明描述了用金刚石样碳（DLC）涂覆聚合物表面的方法，以使其可用作来自神经嵴起源的细胞的载体，优选的形成树突的神经元细胞。 要涂覆DLC的生物聚合物将包括可生物降解的聚合物和其他可植入的生物聚合物，用作细胞移植到身体各个部位的载体系统，包括脑，眼睛，中枢和周围神经系统，肺， 肝，脾，肾，骨和软骨。 生物聚合物可以是片状或微粒形式，并且可以在其合成中嵌入或并入其中，附着或生长促进试剂以增强和支持神经调节附着和生长。 该涂布方法还可以增加其它包被剂，例如由培养的牛角膜内皮细胞分泌的细胞外基质（ECM），以及粘附分子如纤连蛋白，层粘连蛋白和RGDS。 涂覆步骤可以是顺序过程，其中DLC层将被添加到ECM涂覆的表面或附着因子涂覆的表面的顶部上。