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1.发明授权公开(公告)号：US11634457B2
公开(公告)日：2023-04-25
申请号：US16358845
申请日：2019-03-20
发明人： Nanying Bian , Christopher Gillespie , Matthew T. Stone , Mikhail Kozlov , Jie Chen , Martin Siwak
IPC分类号： B01D15/36 , C07K1/36 , C07K1/16 , B01D15/18 , C07K1/18 , C07K1/22 , C07K16/00 , B01J20/282 , C07K1/20 , B01D15/12 , B01J39/26 , B01J41/20 , B01J43/00 , B01J47/04 , B01J20/20 , B01D15/38
摘要： The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.
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公开(公告)号：US11617988B2
公开(公告)日：2023-04-04
申请号：US16207874
申请日：2018-12-03
发明人： Herbert Lutz , Joseph Parrella , Bala Raghunath
摘要： The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.
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公开(公告)号：US11602709B2
公开(公告)日：2023-03-14
申请号：US16328640
申请日：2017-08-29
摘要： Port protection device particularly suited for an encapsulated filter unit. In certain embodiments, the protection device comprises a main body defining an internal region configured to receive a port or the like protruding from an encapsulated filter. In certain embodiments, the main body includes a slot that allows for easy removal of the protection device from the port or the like. A tube management system may be provided to receive and hold tubing. One suitable tube management system includes two spaced tangs that extend from the main body of the protection device and are configured to cooperatively receive and hold tubing between them.
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公开(公告)号：US20220402968A1
公开(公告)日：2022-12-22
申请号：US17756096
申请日：2020-12-11
IPC分类号： C07K1/36 , C12M1/00 , C12N7/00 , C07K1/22 , C07K1/34 , B01D15/38 , B01D15/18 , B01D15/36 , B01D15/32
摘要： Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to filtration to form a clarified product sample; subjecting said clarified product sample to affinity chromatography to remove impurities; subsequently subjecting said product sample to diafiltration followed by virus filtration and optional concentration. The buffer used during the diafiltration step (and thus in the virus filtration step) is the buffer desired for the final formulation of the product.
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公开(公告)号：USD970517S1
公开(公告)日：2022-11-22
申请号：US29758166
申请日：2020-11-12
设计人： Sarah Trice , Lindsey Rickershauser , Xiang Wu , Churl Oh , Nadia Brugger , Emma Gardener
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公开(公告)号：USD962282S1
公开(公告)日：2022-08-30
申请号：US29758161
申请日：2020-11-12
设计人： Sarah Trice , Lindsey Rickershauser , Xiang Wu , Churl Oh , Nadia Brugger , Emma Gardener
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公开(公告)号：US20220186259A1
公开(公告)日：2022-06-16
申请号：US17605190
申请日：2020-06-25
发明人： Naohisa Yoshioka
摘要： Synthetic, self-replicating RNA vectors comprising sequence encoding at least one immortalization protein, and use of the self-replicating RNA vectors to extend the lifespan of primary cell populations and expand said populations of primary cells.
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公开(公告)号：US20220186235A1
公开(公告)日：2022-06-16
申请号：US17606644
申请日：2020-05-13
发明人： Naohisa Yoshioka
摘要： Synthetic, noninfectious, self-replicating RNA vectors that encode CRISPR proteins are provided. Each self-replicating RNA vector comprises a sequence encoding a plurality of non-structural replication complex proteins from an alphavirus and a sequence encoding a CRISPR protein. Also provided are methods for genome editing in which a synthetic self-replicating RNA vector is transfected into cells along with at least one corresponding guide RNA.
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公开(公告)号：US20220176278A1
公开(公告)日：2022-06-09
申请号：US17682221
申请日：2022-02-28
发明人： Jonathan Steen , Mikhail Kozlov , Herbert Lutz , Joseph Parrella , Elizabeth M. Goodrich , Bala Raghunath
摘要： Methods of filtering a liquid feed are disclosed. In one version, the method comprises passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system. In another version, the method of filtering a liquid feed, comprises passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.
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公开(公告)号：US11305271B2
公开(公告)日：2022-04-19
申请号：US15677526
申请日：2017-08-15
发明人： David Yavorsky , John Amara , Joaquin Umana , William Cataldo , Mikhail Kozlov , Matthew Stone
IPC分类号： B01D15/36 , B01J20/28 , B01J47/12 , B01J39/26 , C12N7/02 , B01J47/014 , B01J47/127 , B01D15/38 , C07K1/18 , B01J41/20 , B01D15/32 , B01J20/285 , B01J20/287 , B01J20/32 , C07K16/00 , C12N7/00 , G01N30/52
摘要： Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).
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