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    System for Screening Cells for High Expression of a Protein of Interest (Poi)
    6.
    发明申请
    System for Screening Cells for High Expression of a Protein of Interest (Poi) 审中-公开
    筛选高表达兴趣蛋白（Poi）的细胞系统

    公开(公告)号：US20080286824A1

    公开(公告)日：2008-11-20

    申请号：US12064287

    申请日：2006-08-25

    申请人： Philippe Dupraz ,  Michel Kobr

    发明人： Philippe Dupraz ,  Michel Kobr

    IPC分类号： C12Q1/02 ,  C07K14/00 ,  C07H21/04 ,  C12N15/63 ,  C12P21/04 ,  C12P21/06 ,  C12N15/00 ,  C12N5/08 ,  C12N5/00

    CPC分类号： C12N9/1205 ,  C07K2319/20 ,  C07K2319/60 ,  C12N9/1029 ,  C12N15/64 ,  G01N33/5023

    摘要： This invention refers to industrial production of proteins. More particularly, the invention refers to a fusion protein as a novel chimeric selection marker comprising a peptide conferring resistance to an antibiotic, or a fragment, allelic variant, splice variant or mutein thereof, and at least one sequence comprising SEQ ID NO: 1, 2 or 3, preferably for producing a protein of interest (POI). The inventive chimeric selection marker exhibits: (i) a resistance to an antibiotic; and (ii) a fluorescence activity upon binding of a ligand to the sequence comprising SEQ ID NO: 1, 2 or 3. The invention further refers to nucleic acids encoding the inventive fusion protein and to expression vectors, comprising the inventive fusion protein and additionally the protein of interest (POI). Finally, uses of the inventive chimeric selection marker for screening cells for high expression of a protein of interest (POI) are disclosed.

    摘要翻译： 本发明涉及蛋白质的工业化生产。 更具体地，本发明涉及作为新型嵌合选择标记的融合蛋白，其包含赋予抗生素或其片段等位基因变体，剪接变体或突变蛋白的抗性的肽，以及至少一个包含SEQ ID NO：1， 2或3，优选用于产生感兴趣的蛋白质（POI）。 本发明的嵌合选择标记显示：（i）对抗生素的抗性; 和（ii）在将配体结合到包含SEQ ID NO：1,2或3的序列时的荧光活性。本发明还涉及编码本发明融合蛋白的核酸和包含本发明融合蛋白的另外的表达载体 感兴趣的蛋白质（POI）。 最后，公开了本发明的嵌合选择标记用于筛选高表达目标蛋白（POI）的细胞的用途。

    Pharmaceutically active sulfanilide derivatives
    9.
    发明授权
    Pharmaceutically active sulfanilide derivatives 有权
    药物活性磺酰苯胺衍生物

    公开(公告)号：US07312358B2

    公开(公告)日：2007-12-25

    申请号：US10399040

    申请日：2001-10-15

    申请人： Anna Quattropani ,  Matthias Schwarz ,  Catherine Joran-Lebrun ,  Dennis Church ,  Alexander Scheer

    发明人： Anna Quattropani ,  Matthias Schwarz ,  Catherine Joran-Lebrun ,  Dennis Church ,  Alexander Scheer

    IPC分类号： C07C303/00

    CPC分类号： C07D213/53 ,  A61K31/18 ,  A61K31/4045 ,  A61K31/437 ,  A61K31/4402 ,  A61K31/4439 ,  A61K31/4706 ,  A61K31/472 ,  A61K31/5377 ,  A61K38/00 ,  C07D207/16 ,  C07D209/20 ,  C07D209/40 ,  C07D209/44 ,  C07D217/16 ,  C07D217/26 ,  C07D233/64 ,  C07D471/04 ,  C07K5/06026 ,  C07K5/06191

    摘要： The present invention relates to sulfanilide derivatives of formula (I), in which R1 and R2 are optionally substituted aryl and heteroaryl groups and the other variables are as defined in the claims, for use as pharmaceutically active compounds, as well as pharmaceutical formulations containing such sulfanilide derivatives. Said derivatives are useful in the treatment and/or prevention of preterm labor, premature birth, dysmenorrhea, inappropriate secretion of vasopressin, congestive heart failure, arterial hypertension, liver cirrhosis, nephrotic syndrome and ocular hypertension. In particular, the present invention is related to sulfanilide derivatives displaying a substantial modulatory, in particular antagonistic activity, of the oxytocin and/or vasopressin receptor. More preferably, said compounds are useful in the treatment and/or prevention of disease states mediated by oxytocin and/or vasopressin, including preterm labor, premature birth, dysmenorrhea, inappropriate secretion of vasopressin, congestive heart failure, arterial hypertension, liver cirrhosis, nephrotic syndrome and ocular hypertension. The present invention is furthermore related to novel sulfanilide derivatives as well as to methods of their preparation.

    摘要翻译： 本发明涉及式（I）的磺酰苯胺衍生物，其中R 1和R 2是任选取代的芳基和杂芳基，其它变量如 用作药物活性化合物，以及含有这种磺酰苯胺衍生物的药物制剂。 所述衍生物可用于治疗和/或预防早产，早产，痛经，不适当的血管加压素分泌，充血性心力衰竭，动脉高血压，肝硬化，肾病综合征和高眼压症。 特别地，本发明涉及展示催产素和/或加压素受体的实质性调节，特别是拮抗活性的磺酰苯胺衍生物。 更优选地，所述化合物可用于治疗和/或预防催产素和/或加压素介导的疾病状态，包括早产，早产，痛经，不适当的血管加压素分泌，充血性心力衰竭，动脉高血压，肝硬化，肾病 综合征和高眼压症。 本发明还涉及新的磺酰苯胺衍生物及其制备方法。