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16 条记录 (耗时 0.022 秒)

    Methods of transcriptionally modulating gene expression
    2.
    发明授权
    Methods of transcriptionally modulating gene expression 失效
    转录调控基因表达的方法

    公开(公告)号：US5776502A

    公开(公告)日：1998-07-07

    申请号：US458691

    申请日：1995-06-02

    申请人： J. Gordon Foulkes ,  Robert Franco ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    发明人： J. Gordon Foulkes ,  Robert Franco ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    IPC分类号： C12N15/85 ,  C12Q1/68 ,  C12N15/00 ,  A61K31/70 ,  A61K33/00 ,  A61K49/00

    CPC分类号： A61K31/7105 ,  C12N15/85 ,  C12Q1/6897 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/42 ,  C12N2830/85 ,  C12N2840/20

    摘要： The present invention provides a method of transcriptionally modulating the expression of a gene of interest, the expression of which is associated with a defined physiological or pathological effect within a multicellular organism. The method comprises contacting a cell which is capable of expressing the gene with an amount of a molecule effective to transcriptionally modulate expression of the gene and thereby affect the level of the protein encoded by the gene which is expressed by the cell. Molecules useful in the practice of the invention are characterized as follows (a) do not naturally occur in the cell, (b) specifically transcriptionally modulate expression of the gene of interest, and (c) bind to DNA or RNA or bind to a protein through a domain of such protein which is not a ligand binding domain of a receptor which naturally occurs in the cell, the binding of a ligand to which ligand binding domain is normally associated with the defined physiological or pathological effect.

    摘要翻译： 本发明提供了一种转录调节感兴趣的基因的表达的方法，其表达与多细胞生物体内具有规定的生理或病理作用相关。 该方法包括使能够表达基因的细胞与一定量的有效转录调节基因表达的分子接触，从而影响由细胞表达的基因编码的蛋白质的水平。 在本发明的实践中有用的分子的特征如下（a）天然不发生在细胞中，（b）特异地转录调节目的基因的表达，和（c）结合DNA或RNA或结合蛋白质 通过这种蛋白质的结构域，其不是天然存在于细胞中的受体的配体结合结构域，配体结合结构域通常与所定义的生理或病理作用相关联。

    Detection and quantification of neu related proteins in the biological fluids of humans
    5.
    发明授权
    Detection and quantification of neu related proteins in the biological fluids of humans 失效
    人类生物液体中neu相关蛋白的检测和定量

    公开(公告)号：US5401638A

    公开(公告)日：1995-03-28

    申请号：US806112

    申请日：1991-12-12

    申请人： Walter P. Carney ,  Sara J. McKenzie

    发明人： Walter P. Carney ,  Sara J. McKenzie

    IPC分类号： A61K38/00 ,  C07K14/71 ,  C07K16/32 ,  C12Q1/68 ,  G01N33/574 ,  G01N33/53

    CPC分类号： C07K14/71 ,  C07K16/32 ,  C12Q1/6886 ,  A61K38/00 ,  C12Q2600/158 ,  Y10S436/813

    摘要： This invention relates to a substantially purified p100 which is a human neu related protein having a molecular weight in the range from about 97,000 daltons to about 115,000 daltons which corresponds substantially to the extracellular domain of the human neu gene product, said protein being detectable in a biological fluid.In another embodiment this invention relates to assays for detecting this protein.Finally, this invention also concerns monoclonal antibodies which are capable of binding to p100.

    摘要翻译： 本发明涉及基本上纯化的p100，其是分子量在约97,000道尔顿至约115,000道尔顿范围内的人neu相关蛋白，其基本上对应于人neu基因产物的细胞外结构域，所述蛋白质可在 生物体液。 在另一个实施方案中，本发明涉及用于检测该蛋白质的测定法。 最后，本发明还涉及能够结合p100的单克隆抗体。

    Methods of transcriptionally modulating gene expression and of discovering chemicals capable as gene expression modulators
    6.
    发明授权
    Methods of transcriptionally modulating gene expression and of discovering chemicals capable as gene expression modulators 失效
    转录调控基因表达和发现能够作为基因表达调节剂的化学物质的方法

    公开(公告)号：US5976793A

    公开(公告)日：1999-11-02

    申请号：US683455

    申请日：1996-07-18

    申请人： J. Gordon Foulkes ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    发明人： J. Gordon Foulkes ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    IPC分类号： A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C07K14/775 ,  C12N15/67 ,  C12N15/79 ,  C12N15/85 ,  C12P19/34 ,  C12Q1/68 ,  C12N5/10

    CPC分类号： A61K31/7068 ,  A61K48/00 ,  C07K14/775 ,  C12N15/67 ,  C12Q1/6886 ,  C12Q1/6897 ,  C12Q2600/136

    摘要： The present invention provides a method of transcriptionally modulating the expression of a gene-of-interest. The method comprises contacting a cell which is capable of expressing the gene with an amount of a molecule effective to transcriptionally modulate expression of the gene and thereby affect the level of the protein encoded by the gene which is expressed by the cell. Molecules useful in the practice of the invention are characterized as follows (a) do not naturally occur in the cell, (b) bind to DNA or RNA or bind to a protein through a domain of such protein which is not a ligand binding domain of a receptor which naturally occurs in the cell. Additionally, this invention provides a method for determining whether a molecule known to be a modulator of protein biosynthesis is capable of transcriptionally modulating expression of a gene-of-interest.

    摘要翻译： 本发明提供了转录调节感兴趣基因表达的方法。 该方法包括使能够表达基因的细胞与一定量的有效转录调节基因表达的分子接触，从而影响由细胞表达的基因编码的蛋白质的水平。 在本发明的实践中有用的分子的特征如下（a）天然不发生在细胞中，（b）通过不属于这种蛋白质的配体结合域的蛋白质的结构域结合DNA或RNA或结合蛋白质 天然存在于细胞中的受体。 此外，本发明提供了一种用于确定已知是蛋白质生物合成调节剂的分子是否能够转录调节感兴趣的基因的表达的方法。

    Detection of neu p185 in cell lysates
    8.
    发明授权
    Detection of neu p185 in cell lysates 失效
    检测细胞裂解液中的neu p185

    公开(公告)号：US5604107A

    公开(公告)日：1997-02-18

    申请号：US220962

    申请日：1994-03-30

    申请人： Walter P. Carney ,  Sara J. McKenzie ,  Robert A. Weinberg

    发明人： Walter P. Carney ,  Sara J. McKenzie ,  Robert A. Weinberg

    IPC分类号： A61K38/00 ,  C07K14/71 ,  C07K16/32 ,  C12Q1/68 ,  G01N33/574 ,  G01N33/53

    CPC分类号： C07K14/71 ,  C07K16/32 ,  C12Q1/6886 ,  A61K38/00 ,  C12Q2600/158 ,  Y10S436/813

    摘要： This invention relates to a substantially purified p100 which is a human neu related protein having a molecular weight in the range from about 97,000 daltons to about 115,000 daltons which corresponds substantially to the extracellular domain of the human neu gene product, said protein being detectable in a biological fluid.In another embodiment this invention relates to assays for detecting this protein.Finally, this invention also concerns monoclonal antibodies which are capable of binding to p100.

    摘要翻译： 本发明涉及基本上纯化的p100，其是分子量在约97,000道尔顿至约115,000道尔顿范围内的人neu相关蛋白，其基本上对应于人neu基因产物的细胞外结构域，所述蛋白质可在 生物体液。 在另一个实施方案中，本发明涉及用于检测该蛋白质的测定法。 最后，本发明还涉及能够结合p100的单克隆抗体。

    Detection, quantitation and classification of RAS proteins in body fluids and tissues
    9.
    发明授权
    Detection, quantitation and classification of RAS proteins in body fluids and tissues 失效
    体液和组织中RAS蛋白的检测，定量和分类

    公开(公告)号：US5443956A

    公开(公告)日：1995-08-22

    申请号：US78485

    申请日：1993-06-17

    申请人： Walter P. Carney

    发明人： Walter P. Carney

    IPC分类号： C07K14/82 ,  C07K16/32 ,  G01N33/574 ,  G01N33/68 ,  G01N33/573 ,  G01N33/577

    CPC分类号： C07K14/82 ,  C07K16/32 ,  G01N33/5748 ,  G01N33/6893 ,  Y10S436/813

    摘要： Detection, quantitation and classification of ras p21 proteins in body fluids, tissues, or cells is described. Specifically, this disclosure concerns detecting and quantitating normal and mutated ras p21s from normal subjects, subjects suspected of having preneoplastic disease or subjects known or suspected of having neoplastic (cancer) disease. This invention also concerns the detection and quantitation of the ras p21 proteins into the three families of ras proteins designated Ha, Ki and N in body fluids and tissues.

    摘要翻译： 描述体液，组织或细胞中ras p21蛋白的检测，定量和分类。 具体地，本公开涉及检测和定量正常和突变的ras p21s，来自正常受试者，怀疑患有肿瘤前疾病的受试者或已知或怀疑具有肿瘤（癌症）疾病的受试者。 本发明还涉及将ras p21蛋白检测和定量分析为体液和组织中称为Ha，Ki和N的ras蛋白质的三个家族。