公开(公告)号：US09541553B2

公开(公告)日：2017-01-10

申请号：US14179152

申请日：2014-02-12

Applicant: OREGON HEALTH AND SCIENCE UNIVERSITY

Inventor： Louis Picker ,  Klaus Frueh ,  Scott G. Hansen ,  Collin Powers

IPC: G01N33/569 ,  A61K39/02 ,  A61K39/12 ,  A61K39/00 ,  A61K39/21 ,  A61K39/245 ,  C12N7/00

CPC classification number: G01N33/56972 ,  A61K39/00 ,  A61K39/0011 ,  A61K39/02 ,  A61K39/12 ,  A61K39/21 ,  A61K39/245 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/16121 ,  C12N2710/16122 ,  C12N2710/16131 ,  C12N2710/16134 ,  C12N2710/16143 ,  C12N2710/16162 ,  C12N2710/16171 ,  C12N2710/24143 ,  C12N2740/10034 ,  C12N2740/15034 ,  C12N2740/15043 ,  C12N2800/24

Abstract: This invention also relates to recombinant vectors expressing one or more of the human CMV (HCMV) glycoproteins US2, US3, US6 and US11 or corresponding functional rhesus CMV (RhCMV) homologues Rh182, Rh184, Rh185 or Rh189, methods of making them, uses for them, expression products from them, and uses for the expression products. This invention also relates to recombinant cytomegalovirus vectors vectors lacking one or more of the glycoproteins, methods of making them, uses for them, expression products from them, and uses for the expression products.

Abstract translation: 本发明还涉及表达一种或多种人CMV（HCMV）糖蛋白US2，US3，US6和US11或相应的功能性恒河猴CMV（RhCMV）同系物Rh182，Rh184，Rh185或Rh189的重组载体，其制备方法，用于 他们表达产品，并用于表达产品。 本发明还涉及缺少一种或多种糖蛋白的重组巨细胞病毒载体载体，制备它们的方法，它们的用途，来自它们的表达产物以及用于表达产物的用途。

公开(公告)号：US20160243216A1

公开(公告)日：2016-08-25

申请号：US15033267

申请日：2014-10-28

Applicant: THE UNIVERSITY OF SYDNEY

Inventor： Barry Slobedman ,  Simone Forbes

IPC: A61K39/245 ,  C07K14/565 ,  A61K39/39 ,  C12N7/00

CPC classification number: A61K39/245 ,  A61K39/12 ,  A61K39/39 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/55516 ,  A61K2039/55522 ,  A61K2039/572 ,  C07K14/565 ,  C12N7/00 ,  C12N2710/16121 ,  C12N2710/16132 ,  C12N2710/16134 ,  C12N2710/16151 ,  C12N2710/16162

Abstract: The invention relates to a CMV strain comprising interferon bets (IFNb) useful in immuno-stimulatory compositions and vaccines

Abstract translation: 本发明涉及包含用于免疫刺激组合物和疫苗的干扰素注射（IFNβ）的CMV菌株

公开(公告)号：US09078867B2

公开(公告)日：2015-07-14

申请号：US13695668

申请日：2011-05-05

Applicant: Christian Thirion ,  Ulrich Koszinowski ,  Christian A. Mohr ,  Zsolt Ruzsics

Inventor： Christian Thirion ,  Ulrich Koszinowski ,  Christian A. Mohr ,  Zsolt Ruzsics

IPC: A61K39/245 ,  C12N7/00 ,  C12N15/38 ,  A61K39/00

CPC classification number: A61K39/245 ,  A61K39/12 ,  A61K2039/525 ,  A61K2039/5254 ,  A61K2039/575 ,  C12N7/00 ,  C12N2710/16111 ,  C12N2710/16121 ,  C12N2710/16134 ,  C12N2710/16152 ,  C12N2710/16162 ,  C12N2710/16171 ,  C12N2710/16511 ,  C12N2830/003

Abstract: The present invention is related to a beta-herpesvirus, wherein the beta-herpesvirus is spread-deficient.

Abstract translation: 本发明涉及β-疱疹病毒，其中β-疱疹病毒是扩散缺陷型的。

公开(公告)号：US5906935A

公开(公告)日：1999-05-25

申请号：US946598

申请日：1997-10-07

Applicant: Thomas R. Jones ,  Ann E. Campbell

Inventor： Thomas R. Jones ,  Ann E. Campbell

IPC: C12N15/00 ,  A61K31/70 ,  A61K35/76 ,  A61K38/00 ,  A61K39/245 ,  A61K48/00 ,  A61P31/12 ,  C07K14/045 ,  C12N7/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/01 ,  C12N15/09 ,  C12N15/34 ,  C12N15/38 ,  C12N15/86 ,  C12N15/861 ,  C12R1/92 ,  C12N5/10 ,  C12N1/00 ,  C12N15/79

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/245 ,  C12N15/86 ,  C12N7/00 ,  A61K2039/5156 ,  A61K2039/5254 ,  A61K38/00 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2710/16121 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12N2710/16143 ,  C12N2710/16151 ,  C12N2710/16161 ,  C12N2710/16162

Abstract: Infection of human fibroblast cells with human cytomegalovirus (HCMV) causes down regulation of cell surface expression of MHC class I. The present invention is directed to a mutant with a 9-kb deletion in the S component of the HCMV genome (including open reading frames IRS1-US9 and US11) which failed to down regulate class I heavy chains. By examining the phenotypes of mutants with smaller deletions with this portion of the HCMV genome, a 7-kb region containing at least 9 open reading frames was shown to contain the genes required for reduction in heavy chain expression. Furthermore, it was determined that two subregions (A and B) of the 7-kb region each contained genes which were sufficient to cause heavy chain down regulation. In subregion B, the US11 gene product is involved. It encodes a endoglycosidase H-sensitive glycoprotein which is intracytoplasmic, similar to the adenovirus type 2 E3-19K glycoprotein which inhibits surface expression of class I heavy chains.

Abstract translation: 用人巨细胞病毒（HCMV）感染人成纤维细胞导致I类MHC细胞表面表达的下调。本发明涉及在HCMV基因组的S成分中具有9-kb缺失的突变体（包括开放阅读框 IRS1-US9和US11），其不能下调I类重链。 通过使用该部分HCMV基因组检查具有较小缺失的突变体的表型，显示含有至少9个开放阅读框的7-kb区域含有重链表达降低所需的基因。 此外，确定了7-kb区域的两个亚区（A和B）各自含有足以引起重链下调的基因。 在次区域B中，涉及US11基因产物。 它编码胞质内H敏感的糖蛋白，其是胞质内的，类似于抑制I类重链表面表达的2型E3-19K糖蛋白。

公开(公告)号：US5843458A

公开(公告)日：1998-12-01

申请号：US509214

申请日：1995-07-31

Applicant: Thomas R. Jones

Inventor： Thomas R. Jones

IPC: C12N15/00 ,  A61K31/70 ,  A61K35/76 ,  A61K38/00 ,  A61K39/245 ,  A61K48/00 ,  A61P31/12 ,  C07K14/045 ,  C12N7/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/01 ,  C12N15/09 ,  C12N15/34 ,  C12N15/38 ,  C12N15/86 ,  C12N15/861 ,  C12R1/92 ,  C12N15/63

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/245 ,  C12N15/86 ,  C12N7/00 ,  A61K2039/5156 ,  A61K2039/5254 ,  A61K38/00 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2710/16121 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12N2710/16143 ,  C12N2710/16151 ,  C12N2710/16161 ,  C12N2710/16162

Abstract: Infection of human fibroblast cells with human cytomegalovirus (HCMV) causes down-regulation of cell surface expression of MHC class I. A recombinant mutant HCMV which fails to down-regulate class I heavy chain expression is described. A method of controlling down-regulation of MHC class I expression in a cytomegalovirus infected cell, a pharmaceutical composition, a vaccine composition, a method of preventing or reducing susceptibility to acute cytomegalovirus in an individual, and a virus based gene therapy vector are also described.

Abstract translation: 用人巨细胞病毒（HCMV）感染人成纤维细胞导致MHC类I细胞表面表达下调。描述了不能下调I型重链表达的重组突变体HCMV。 还描述了控制巨细胞病毒感染细胞中MHC I类表达下调的方法，药物组合物，疫苗组合物，预防或降低个体中急性巨细胞病毒敏感性的方法和基于病毒的基因治疗载体 。

公开(公告)号：US09862972B2

公开(公告)日：2018-01-09

申请号：US14086602

申请日：2013-11-21

Applicant: Oregon Health & Science University

Inventor： Louis Picker ,  Klaus Früh ,  Scott Hansen

IPC: C12N15/86 ,  C12N7/00 ,  A61K39/12 ,  A61K39/00

CPC classification number: C12N15/86 ,  A61K39/12 ,  A61K2039/5256 ,  C12N7/00 ,  C12N2710/16121 ,  C12N2710/16143 ,  C12N2740/15034 ,  C12N2740/15071 ,  C12N2800/204 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/394 ,  Y02A50/412 ,  Y02A50/466

Abstract: Disclosed herein are recombinant CMV vectors which may comprise a heterologous antigen that can repeatedly infect an organism while inducing a CD8+ T cell response to immunodominant epitopes of the heterologous antigen. The CMV vector may comprise a deleterious mutation in the US11 glycoprotein or a homolog thereof.

公开(公告)号：US20170274057A1

公开(公告)日：2017-09-28

申请号：US15011601

申请日：2016-01-31

Applicant: Stipan JONJIC

Inventor： Stipan JONJIC

IPC: A61K39/00 ,  A61K39/02 ,  A61K39/04 ,  A61K39/12 ,  C12N7/00 ,  C12N7/04

CPC classification number: A61K39/00 ,  A61K39/0208 ,  A61K39/04 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N7/045 ,  C12N2710/16121 ,  C12N2710/16134 ,  C12N2710/16143 ,  C12N2710/16162 ,  C12N2740/16234 ,  C12N2760/16134 ,  C12N2760/18534 ,  Y02A50/412

Abstract: The present invention relates to a beta-herpesvirus, preferably a recombinant beta-herpesvirus, wherein the beta-herpesvirus comprises at least one heterologous nucleic acid, wherein the at least one heterologous nucleic acid comprises a gene encoding a cellular ligand.

公开(公告)号：US09395369B2

公开(公告)日：2016-07-19

申请号：US14059309

申请日：2013-10-21

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： Mark Schleiss

IPC: A61K39/245 ,  C12Q1/70 ,  G01N33/569 ,  C12N7/00 ,  A61K39/00

CPC classification number: G01N33/56994 ,  A61K39/00 ,  C12N7/00 ,  C12N2710/16121 ,  G01N2500/00

Abstract: The present disclosure provides an isolated or purified guinea pig cytomegalovirus (GPCMV) Strain CIDMTR, glycoproteins from GPCMV Strain CIDMTR, and methods of use thereof.

Abstract translation: 本公开提供了分离或纯化的豚鼠巨细胞病毒（GPCMV）菌株CIDMTR，来自GPCMV菌株CIDMTR的糖蛋白及其使用方法。

公开(公告)号：US20140302530A1

公开(公告)日：2014-10-09

申请号：US14179152

申请日：2014-02-12

Applicant: OREGON HEALTH AND SCIENCE UNIVERSITY

Inventor： Louis Picker ,  Klaus Frueh ,  Scott G. Hansen ,  Collin Powers

IPC: G01N33/569

CPC classification number: G01N33/56972 ,  A61K39/00 ,  A61K39/0011 ,  A61K39/02 ,  A61K39/12 ,  A61K39/21 ,  A61K39/245 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/16121 ,  C12N2710/16122 ,  C12N2710/16131 ,  C12N2710/16134 ,  C12N2710/16143 ,  C12N2710/16162 ,  C12N2710/16171 ,  C12N2710/24143 ,  C12N2740/10034 ,  C12N2740/15034 ,  C12N2740/15043 ,  C12N2800/24

Abstract: This invention also relates to recombinant vectors expressing one or more of the human CMV (HCMV) glycoproteins US2, US3, US6 and US11 or corresponding functional rhesus CMV (RhCMV) homologues Rh182, Rh184, Rh185 or Rh189, methods of making them, uses for them, expression products from them, and uses for the expression products. This invention also relates to recombinant cytomegalovirus vectors vectors lacking one or more of the glycoproteins, methods of making them, uses for them, expression products from them, and uses for the expression products.

Abstract translation: 本发明还涉及表达一种或多种人CMV（HCMV）糖蛋白US2，US3，US6和US11或相应的功能性恒河猴CMV（RhCMV）同系物Rh182，Rh184，Rh185或Rh189的重组载体，其制备方法，用于 他们表达产品，并用于表达产品。 本发明还涉及缺少一种或多种糖蛋白的重组巨细胞病毒载体载体，制备它们的方法，它们的用途，来自它们的表达产物以及用于表达产物的用途。

公开(公告)号：US5720957A

公开(公告)日：1998-02-24

申请号：US459586

申请日：1995-06-02

Applicant: Thomas R. Jones ,  Ann E. Campbell

Inventor： Thomas R. Jones ,  Ann E. Campbell

IPC: C12N15/00 ,  A61K31/70 ,  A61K35/76 ,  A61K38/00 ,  A61K39/245 ,  A61K48/00 ,  A61P31/12 ,  C07K14/045 ,  C12N7/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/01 ,  C12N15/09 ,  C12N15/34 ,  C12N15/38 ,  C12N15/86 ,  C12N15/861 ,  C12R1/92

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/245 ,  C12N15/86 ,  C12N7/00 ,  A61K2039/5156 ,  A61K2039/5254 ,  A61K38/00 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2710/16121 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12N2710/16143 ,  C12N2710/16151 ,  C12N2710/16161 ,  C12N2710/16162

Abstract: Infection of human fibroblast cells with human cytomegalovirus (HCMV) causes down regulation of cell surface expression of MHC class I. The present invention is directed to a mutant with a 9-kb deletion in the S component of the HCMV genome (including open reading frames IRS1-US9 and US11) which failed to down regulate class I heavy chains. By examining the phenotypes of mutants with smaller deletions with this portion of the HCMV genome, a 7-kb region containing at least 9 open reading frames was shown to contain the genes required for reduction in heavy chain expression. Furthermore, it was determined that two subregions (A and B) of the 7-kb region each contained genes which were sufficient to cause heavy chain down regulation. In subregion B, the US11 gene product is involved. It encodes a endoglycosidase H-sensitive glycoprotein which is intracytoplasmic, similar to the adenovirus type 2 E3-19K glycoprotein which inhibits surface expression of class I heavy chains.

Abstract translation: 用人巨细胞病毒（HCMV）感染人成纤维细胞导致I类MHC细胞表面表达的下调。本发明涉及在HCMV基因组的S成分中具有9-kb缺失的突变体（包括开放阅读框 IRS1-US9和US11），其不能下调I类重链。 通过使用该部分HCMV基因组检查具有较小缺失的突变体的表型，显示含有至少9个开放阅读框的7-kb区域含有重链表达降低所需的基因。 此外，确定了7-kb区域的两个亚区（A和B）各自含有足以引起重链下调的基因。 在次区域B中，涉及US11基因产物。 它编码胞质内H敏感的糖蛋白，其是胞质内的，类似于抑制I类重链表面表达的2型E3-19K糖蛋白。