公开(公告)号：US20240309417A1

公开(公告)日：2024-09-19

申请号：US18413954

申请日：2024-01-16

申请人： Ramon Gonzalez

发明人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

IPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/00 ,  C12P13/04

CPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0077 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P5/02 ,  C12P5/026 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/001 ,  C12P13/04 ,  C12Y101/01 ,  C12Y101/03039 ,  C12Y102/01 ,  C12Y102/04001 ,  C12Y103/05001 ,  C12Y103/99 ,  C12Y108/01004 ,  C12Y114/15003 ,  C12Y203/01012 ,  C12Y203/01054 ,  C12Y207/01031 ,  C12Y208/03 ,  C12Y401/00 ,  C12Y401/01 ,  C12Y401/01047 ,  C12Y401/03024 ,  C12Y402/01 ,  C12Y402/01002 ,  C12Y402/01011 ,  C12Y501/99001 ,  C12Y501/99002 ,  C12Y504/02 ,  C12Y504/99 ,  C12Y504/99001 ,  C12Y504/99002 ,  C12Y602/01 ,  C12Y602/01005 ,  C12Y604/01003 ,  Y02E50/30

摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

公开(公告)号：US11913049B2

公开(公告)日：2024-02-27

申请号：US16878032

申请日：2020-05-19

申请人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

发明人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

IPC分类号： C12N9/00 ,  C12P19/32 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/46 ,  C12N9/04 ,  C12N9/02 ,  C12P7/625 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/42 ,  C12P9/00 ,  C12P13/00 ,  C12P13/04

CPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/001 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0077 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P5/02 ,  C12P5/026 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/001 ,  C12P13/04 ,  C12Y101/01 ,  C12Y101/03039 ,  C12Y102/01 ,  C12Y102/04001 ,  C12Y103/05001 ,  C12Y103/99 ,  C12Y108/01004 ,  C12Y114/15003 ,  C12Y203/01012 ,  C12Y203/01054 ,  C12Y207/01031 ,  C12Y208/03 ,  C12Y401/00 ,  C12Y401/01 ,  C12Y401/01047 ,  C12Y401/03024 ,  C12Y402/01 ,  C12Y402/01002 ,  C12Y402/01011 ,  C12Y501/99001 ,  C12Y501/99002 ,  C12Y504/02 ,  C12Y504/99 ,  C12Y504/99001 ,  C12Y504/99002 ,  C12Y602/01 ,  C12Y602/01005 ,  C12Y604/01003 ,  Y02E50/30

摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

公开(公告)号：US20180355394A1

公开(公告)日：2018-12-13

申请号：US15562606

申请日：2016-03-31

申请人： Ramon Gonzales

发明人： Ramon Gonzales

IPC分类号： C12P19/32 ,  C12N9/02 ,  C12N9/04 ,  C12N9/00 ,  C12N9/88 ,  C12N9/10 ,  C12N9/90 ,  C12P7/40 ,  C12P7/46 ,  C12P7/42 ,  C12P9/00 ,  C12P7/02 ,  C12P7/24 ,  C12P5/02 ,  C12P13/00 ,  C12P13/04

CPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0077 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P5/02 ,  C12P5/026 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/001 ,  C12P13/04 ,  C12Y101/01 ,  C12Y101/03039 ,  C12Y102/01 ,  C12Y102/04001 ,  C12Y103/05001 ,  C12Y103/99 ,  C12Y108/01004 ,  C12Y114/15003 ,  C12Y203/01012 ,  C12Y203/01054 ,  C12Y207/01031 ,  C12Y208/03 ,  C12Y401/00 ,  C12Y401/01 ,  C12Y401/01047 ,  C12Y401/03024 ,  C12Y402/01 ,  C12Y402/01002 ,  C12Y402/01011 ,  C12Y501/99001 ,  C12Y501/99002 ,  C12Y504/02 ,  C12Y504/99 ,  C12Y504/99001 ,  C12Y504/99002 ,  C12Y602/01 ,  C12Y602/01005 ,  C12Y604/01003 ,  Y02E50/343

摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.