公开(公告)号：US10006033B2

公开(公告)日：2018-06-26

申请号：US14853946

申请日：2015-09-14

申请人： The Regents of the University of California

发明人： James C. Liao ,  Igor Bogorad

IPC分类号： C12P7/16 ,  C12N1/20 ,  C12P7/18 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C07K1/00 ,  C12N15/52 ,  C12P7/06 ,  C12N9/10 ,  C12N9/88

CPC分类号： C12N15/52 ,  C12N9/1022 ,  C12N9/88 ,  C12P7/06 ,  C12P7/16 ,  C12Y202/01003 ,  C12Y401/02009 ,  C12Y401/02022 ,  C12Y401/02043 ,  Y02E50/10 ,  Y02E50/17

摘要： Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.

公开(公告)号：US20160376600A1

公开(公告)日：2016-12-29

申请号：US15038922

申请日：2014-11-25

申请人： GENOMATICA, INC.

发明人： Robin E. OSTERHOUT ,  Anthony P. BURGARD ,  Priti PHARKYA ,  Stefan ANDRAE

IPC分类号： C12N15/52 ,  C12N9/04 ,  C12N9/90 ,  C12N9/10 ,  C12P7/04 ,  C12N9/88

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12Y101/01244 ,  C12Y202/01003 ,  C12Y401/02043 ,  C12Y503/01027

摘要： The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

摘要翻译： 本发明提供具有甲醛固定通道，甲酸同化途径和/或与脂肪醇，脂肪醛，脂肪酸或异丙醇途径组合的甲醇代谢途径的非天然存在的微生物生物，其中所述微生物有选择地产生 脂肪醇，脂肪醛或指定长度的脂肪酸或异丙醇。 提供的微生物有利地增强了用于生产链长特异性脂肪醇，脂肪醛，脂肪酸或异丙醇的底物和/或途径中间体的生产。 在一些方面，本发明的微生物有选择性的基因破坏或酶减弱，其增加脂肪醇，脂肪醛或脂肪酸的产生。 本发明另外提供了使用上述微生物生产脂肪醇，脂肪醛，脂肪酸或异丙醇的方法。

公开(公告)号：US20160060635A1

公开(公告)日：2016-03-03

申请号：US14853946

申请日：2015-09-14

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： James C. Liao ,  Igor Bogorad

IPC分类号： C12N15/52 ,  C12P7/62

CPC分类号： C12N15/52 ,  C12N9/1022 ,  C12N9/88 ,  C12P7/06 ,  C12P7/16 ,  C12Y202/01003 ,  C12Y401/02009 ,  C12Y401/02022 ,  C12Y401/02043 ,  Y02E50/10 ,  Y02E50/17

摘要： Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.

摘要翻译： 提供了催化从甲醇，甲烷和/或甲醛合成化学物质和生物化学物质的微生物。 还提供了产生这种生物体的方法和使用这种生物体合成化学品和生物化学品的方法。