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    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL
    1.
    发明申请
    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL 审中-公开
    在甲醇存在下增强特定长度脂肪醇的微生物生产方法

    公开(公告)号：US20160376600A1

    公开(公告)日：2016-12-29

    申请号：US15038922

    申请日：2014-11-25

    Applicant: GENOMATICA, INC.

    Inventor： Robin E. OSTERHOUT ,  Anthony P. BURGARD ,  Priti PHARKYA ,  Stefan ANDRAE

    IPC: C12N15/52 ,  C12N9/04 ,  C12N9/90 ,  C12N9/10 ,  C12P7/04 ,  C12N9/88

    CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12Y101/01244 ,  C12Y202/01003 ,  C12Y401/02043 ,  C12Y503/01027

    Abstract: The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

    Abstract translation: 本发明提供具有甲醛固定通道，甲酸同化途径和/或与脂肪醇，脂肪醛，脂肪酸或异丙醇途径组合的甲醇代谢途径的非天然存在的微生物生物，其中所述微生物有选择地产生 脂肪醇，脂肪醛或指定长度的脂肪酸或异丙醇。 提供的微生物有利地增强了用于生产链长特异性脂肪醇，脂肪醛，脂肪酸或异丙醇的底物和/或途径中间体的生产。 在一些方面，本发明的微生物有选择性的基因破坏或酶减弱，其增加脂肪醇，脂肪醛或脂肪酸的产生。 本发明另外提供了使用上述微生物生产脂肪醇，脂肪醛，脂肪酸或异丙醇的方法。

    ALCOHOL DEHYDROGENASE VARIANTS
    4.
    发明公开
    ALCOHOL DEHYDROGENASE VARIANTS 审中-公开

    公开(公告)号：US20230159902A1

    公开(公告)日：2023-05-25

    申请号：US17861036

    申请日：2022-07-08

    Applicant: Genomatica, Inc.

    Inventor： Stefan ANDRAE ,  Michael Patrick KUCHINSKAS ,  Jingyi LI ,  Harish NAGARAJAN ,  Priti PHARKYA

    IPC: C12N9/04 ,  C12P7/24 ,  C12P7/18

    CPC classification number: C12N9/0006 ,  C12P7/24 ,  C12Y101/01244 ,  C12P7/18 ,  Y02P20/10 ,  C12Y101/01001

    Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS
    6.
    发明申请
    MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS 审中-公开

    公开(公告)号：US20170159075A1

    公开(公告)日：2017-06-08

    申请号：US15039221

    申请日：2014-11-25

    Applicant: GENOMATICA, INC.

    Inventor： Robin E. OSTERHOUT ,  Anthony P. BURGARD ,  Priti PHARKYA ,  Stefan ANDRAE

    IPC: C12P5/02 ,  C12P7/04 ,  C12N15/81 ,  C12P17/10 ,  C12P13/00 ,  C12N15/70 ,  C12P7/18 ,  C12P7/44

    Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

    MICROORGANISMS AND METHODS FOR PRODUCING BUTADIENE AND RELATED COMPOUNDS BY FORMATE ASSIMILATION
    8.
    发明申请
    MICROORGANISMS AND METHODS FOR PRODUCING BUTADIENE AND RELATED COMPOUNDS BY FORMATE ASSIMILATION 审中-公开
    微生物和通过正式评估生产丁二烯及相关化合物的方法

    公开(公告)号：US20150050708A1

    公开(公告)日：2015-02-19

    申请号：US14213806

    申请日：2014-03-14

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Stefan ANDRAE

    IPC: C12P5/02 ,  C12N15/81 ,  C12P7/16 ,  C12N15/70 ,  C12P7/04 ,  C12P7/18

    CPC classification number: C12P5/026 ,  C12N15/52 ,  C12P7/04 ,  C12P7/16 ,  C12P7/18 ,  Y02E50/10 ,  Y02E50/343

    Abstract: Provided herein are non-naturally occurring microbial organisms having a formaldehyde fixation pathway and a formate assimilation pathway, which can further include a methanol metabolic pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. These microbial organisms can further include a butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol.

    Abstract translation: 本文提供了具有甲醛固定途径和甲酸同化途径的非天然存在的微生物生物，其可以进一步包括甲醇代谢途径，甲醇氧化途径，氢化酶和/或一氧化碳脱氢酶。 这些微生物可进一步包括丁二烯，1,3-丁二醇，巴豆醇或3-丁烯-2-醇途径。 另外提供了使用这些微生物生产丁二烯，1,3-丁二醇，巴豆醇或3-丁烯-2-醇的方法。

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