Production of wheat protein hydrolyzates by multistage hydrolysis with a
proteinase and peptidase
    7.
    发明授权
    Production of wheat protein hydrolyzates by multistage hydrolysis with a proteinase and peptidase 失效
    用蛋白酶和肽酶多级水解生产小麦蛋白水解产物

    公开(公告)号:US5945299A

    公开(公告)日:1999-08-31

    申请号:US875677

    申请日:1997-10-06

    摘要: Wheat protein hydrolyzates are produced by hydrolyzing a wheat protein-containing starting material, preferably a wheat protein isolate having a protein content of 70 to 90%, with a proteinase such as a serine protease at a pH of 2 to 5, then with a proteinase at a pH of 8 to 10 and finally with a peptidase at a pH of 6 to 7. The hydrolysis steps are preferably at a temperature below the gelatinization temperature of carbohydrate present in the protein. Each hydrolysis may be carried out for 1 to 24 hours at a temperature of 40 to 70.degree. C. To reduce traces of unwanted color formers, an adsorbent such as silica gel, aluminum oxide or activated carbon may be present during the hydrolysis steps. Aqueous wheat protein hydrolyzate solutions are obtained and, if required, may be concentrated, for example using falling film evaporators. The hydrolyzates have an average molecular weight in the range of 100 to 30,000, preferably 2,000 to 5,000, and a solids content of around 5 to 50%. The hydrolyzates may be condensed with a fatty acid or a fatty acid chloride containing 6 to 22 carbons such as lauric acid or coconut oil fatty acid to produce light-colored, storage-stable derivatives.

    摘要翻译: PCT No.PCT / EP96 / 00147 Sec。 371日期:1997年10月6日 102(e)日期1997年10月6日PCT PCT 1996年1月16日PCT公布。 第WO96 / 22699号公报 日期1996年8月1日蛋白质水解产物是通过在蛋白质酶如丝氨酸蛋白酶的pH为2至5下水解具有蛋白质含量为70至90%的小麦蛋白质起始物质,优选蛋白质分解物, 然后用pH为8至10的蛋白酶,最后用pH6-7的肽酶进行。水解步骤优选在低于蛋白质中碳水化合物凝胶化温度的温度。 每个水解可以在40至70℃的温度下进行1至24小时。为了减少痕量的不需要的成色剂,在水解步骤期间可能存在诸如硅胶,氧化铝或活性炭的吸附剂。 获得水性小麦蛋白水解物溶液,如果需要,可以浓缩,例如使用降膜蒸发器。 水解产物的平均分子量为100至30,000,优选为2,000至5,000,固体含量为约5至50%。 水解产物可以与脂肪酸或含有6-22个碳原子的脂肪酰氯例如月桂酸或椰子油脂肪酸缩合,产生浅色的储存稳定的衍生物。