公开(公告)号：US11834711B2

公开(公告)日：2023-12-05

申请号：US17484674

申请日：2021-09-24

申请人： Karius, Inc.

发明人： Timothy A Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC分类号： C12Q1/6869 ,  C12N9/00 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12N9/12 ,  C12Q1/686 ,  C12Q1/6876

CPC分类号： C12Q1/6869 ,  C12N9/1247 ,  C12N9/93 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6855 ,  C12Q1/6876 ,  C12Q2521/107 ,  C12Q2533/101 ,  C12Y207/07006 ,  C12Y207/07049 ,  C12Y605/01001 ,  C12Y605/01003 ,  C12Q1/6806 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2535/122

摘要： The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US20160002716A1

公开(公告)日：2016-01-07

申请号：US14839433

申请日：2015-08-28

申请人： New England Biolabs, Inc.

发明人： Gregory Lohman ,  Thomas C. Evans, JR. ,  Larry A. McReynolds

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6862 ,  C12N9/93 ,  C12Q1/6853 ,  C12Y605/01003 ,  G01N2333/9015 ,  C12Q2521/501 ,  C12Q2561/125

摘要： Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.

摘要翻译： 提供组合物和方法用于在RNA夹板上连接长度大于8个核苷酸的多核苷酸。 连接反应在高或低ATP浓度下提供一致的结果。 该反应可以快速发生，并且在T4DNA连接酶的最佳条件下通常比T4DNA连接酶的效力至少高10倍，反应时间小于6小时，例如小于1小时。

公开(公告)号：US20120252691A1

公开(公告)日：2012-10-04

申请号：US13482927

申请日：2012-05-29

申请人： Christopher L. Etienne ,  Kay K. Opperman ,  Barbara J. Kaboord ,  Scott Meier ,  Jean-Samuel Schultz

发明人： Christopher L. Etienne ,  Kay K. Opperman ,  Barbara J. Kaboord ,  Scott Meier ,  Jean-Samuel Schultz

IPC分类号： C07H19/10 ,  C12Q1/68 ,  C40B30/04 ,  G01N33/566 ,  C07K17/10 ,  C07H21/02

CPC分类号： C12Q1/6876 ,  C07H19/10 ,  C07H19/20 ,  C12P19/34 ,  C12Q1/6816 ,  C12Y605/01001 ,  C12Y605/01002 ,  C12Y605/01003 ,  C12Q2525/117 ,  C12Q2563/131

摘要： Modified nucleotides, and methods to modify nucleotides with a moiety or label, such as biotin, that permits their detection and results in a modified nucleotide, and methods of use of the modified nucleotide in quantitative and qualitative assays.

摘要翻译： 修饰的核苷酸以及修饰具有部分或标记（例如生物素）的核苷酸的方法，其允许其检测并产生修饰的核苷酸，以及在定量和定性测定中使用经修饰的核苷酸的方法。

公开(公告)号：US20240294979A1

公开(公告)日：2024-09-05

申请号：US18486005

申请日：2023-10-12

申请人： Karius, Inc.

发明人： Timothy A. Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC分类号： C12Q1/6869 ,  C12N9/00 ,  C12N9/12 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876

CPC分类号： C12Q1/6869 ,  C12N9/1247 ,  C12N9/93 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2521/107 ,  C12Q2533/101 ,  C12Y207/07006 ,  C12Y207/07049 ,  C12Y605/01001 ,  C12Y605/01003

摘要： The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US20180127822A1

公开(公告)日：2018-05-10

申请号：US15792875

申请日：2017-10-25

申请人： Pierce Biotechnology, Inc.

发明人： Christopher L. Etienne ,  Kay K. Opperman ,  Barbara J. Kaboord ,  Scott D. Meier ,  Jean-Samuel Schultz

IPC分类号： C12Q1/6876 ,  C12P19/34 ,  C07H19/10 ,  C07H19/20

CPC分类号： C12Q1/6876 ,  C07H19/10 ,  C07H19/20 ,  C12P19/34 ,  C12Q1/6816 ,  C12Y605/01001 ,  C12Y605/01002 ,  C12Y605/01003 ,  C12Q2525/117 ,  C12Q2563/131

摘要： Modified nucleotides, and methods to modify nucleotides with a moiety or label, such as biotin, that permits their detection and results in a modified nucleotide, and methods of use of the modified nucleotide in quantitative and qualitative assays.

公开(公告)号：US20140179539A1

公开(公告)日：2014-06-26

申请号：US13829489

申请日：2013-03-14

申请人： New England Biolabs, Inc.

发明人： Gregory Lohman ,  Thomas C. Evans

IPC分类号： C12Q1/68 ,  C12N9/00

CPC分类号： C12N9/93 ,  C12N9/00 ,  C12Q1/6853 ,  C12Q1/6862 ,  C12Q2521/501 ,  C12Q2561/125 ,  C12Y605/01003

摘要： Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.

摘要翻译： 提供组合物和方法用于在RNA夹板上连接长度大于8个核苷酸的多核苷酸。 连接反应在高或低ATP浓度下提供一致的结果。 该反应可以快速发生，并且在T4DNA连接酶的最佳条件下通常比T4DNA连接酶的效力至少高10倍，反应时间小于6小时，例如小于1小时。

公开(公告)号：US20240277872A1

公开(公告)日：2024-08-22

申请号：US18560348

申请日：2022-05-11

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Xiao Wang ,  Hailing Shi ,  Abhishek Aditham

IPC分类号： A61K48/00 ,  A61K38/53 ,  C12N15/10 ,  C12N15/67

CPC分类号： A61K48/005 ,  A61K38/53 ,  C12N15/1013 ,  C12N15/67 ,  C12Y605/01003 ,  C12N2310/313 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/336

摘要： Disclosed herein are modified niRNAs and modified non-coding RNAs with poly(A) tails containing modified nucleotides and/or secondary structures, which may be made by ligation of a tailing nucleic acid onto the 3′ terminal end of an RNA. Also provided are compositions comprising one or more modified mRNAs or modified non-coding RNAs provided herein, and methods of using said compositions for therapeutic or agricultural applications.

公开(公告)号：US20240182968A1

公开(公告)日：2024-06-06

申请号：US18438896

申请日：2024-02-12

申请人： 10x Genomics, Inc.

发明人： Felice Alessio Bava

IPC分类号： C12Q1/6876 ,  C12Q1/6837

CPC分类号： C12Q1/6876 ,  C12Q1/6837 ,  C12Q2521/507 ,  C12Q2525/173 ,  C12Q2531/125 ,  C12Y605/01001 ,  C12Y605/01003

摘要： This disclosure relates to methods for increasing capture efficiency of a spatial array using rolling circle amplification of a padlock probe that hybridizes to a capture probe. Also provided are methods for using such spatial arrays to detect a biological analyte in a biological sample.

公开(公告)号：US20240076748A1

公开(公告)日：2024-03-07

申请号：US18455201

申请日：2023-08-24

申请人： INSTITUT PASTEUR DE MONTEVIDEO ,  UNIVERSIDAD DE LA REPÚBLICA ,  JOHNS HOPKINS UNIVERSITY

发明人： Juan Pablo Tosar ,  Bruno Costa ,  Alfonso Cayota ,  Kenneth Witwer

IPC分类号： C12Q1/6886 ,  C12N9/00 ,  C12N9/12 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/686

CPC分类号： C12Q1/6886 ,  C12N9/1205 ,  C12N9/93 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Y207/01078 ,  C12Y605/01003

摘要： The present disclosure relates to a method of enzymatically repairing RNAs that are nicked or at least partially cleaved. The method comprises providing a biological sample containing RNAs that are nicked or at least partially cleaved; purifying the nicked or at least partially cleaved RNAs contained in the biological sample, under a non-denaturing condition, to remove non-RNA components; and treating the purified RNAs with at least one of the following: (i) one or more enzymes that exhibit the activity of an RNA 3′ phosphatase or cyclic phosphatase and the activity of an RNA 5′ kinase, and an RNA ligase, or (ii) a 3′-5′ RNA ligase, thereby forming repaired RNAs from the nicked or at least partially cleaved RNA. The present disclosure also relates to a method of detecting RNAs by enzymatically repairing RNAs that are nicked or at least partially cleaved, and detecting the repaired RNAs.

公开(公告)号：US09828633B1

公开(公告)日：2017-11-28

申请号：US14883810

申请日：2015-10-15

申请人： Pierce Biotechnology, Inc.

发明人： Christopher L. Etienne ,  Kay K. Opperman ,  Barbara J. Kaboord ,  Scott D. Meier ,  Jean-Samuel Schultz

IPC分类号： C07H19/20 ,  C07H19/10 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C07H19/10 ,  C07H19/20 ,  C12P19/34 ,  C12Q1/6816 ,  C12Y605/01001 ,  C12Y605/01002 ,  C12Y605/01003 ,  C12Q2525/117 ,  C12Q2563/131

摘要： Modified nucleotides, and methods to modify nucleotides with a moiety or label, such as biotin, that permits their detection and results in a modified nucleotide, and methods of use of the modified nucleotide in quantitative and qualitative assays.