公开(公告)号：US12089574B2

公开(公告)日：2024-09-17

申请号：US16952807

申请日：2020-11-19

申请人： Regents of the University of Minnesota

发明人： Daniel J. Garry ,  Mary G. Garry ,  Naoko Koyano

IPC分类号： A01K67/0271 ,  A01K67/0276 ,  C12N5/073 ,  C12N5/077 ,  A61K35/34

CPC分类号： A01K67/0271 ,  A01K67/0276 ,  C12N5/0604 ,  C12N5/0658 ,  A01K2217/00 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/108 ,  A01K2267/025 ,  A61K35/34 ,  C12N2510/00 ,  C12N2517/02

摘要： Described herein is a method for producing a chimeric non-human animal expressing a human a MYF5, MYOD, MRF4 gene or a combination thereof gene comprising: a) generating an MYF5, MYOD, MRF4 or combination thereof null non-human animal cell, wherein both copies of the non-human MYF5, MYOD, MRF4 gene or combination thereof carry a mutation that prevents production of functional MYF5, MYOD, MRF4 protein or combination thereof in said non-human animal; b) creating a MYF5, MYOD, MRF4 or combination thereof null non-human blastocyst by somatic cell nuclear transfer comprising fusing a nucleus from said MYF5, MYOD, MRF4 or combination thereof null non-human animal cell of a) into an enucleated non-human oocyte and activating said oocyte to divide so as to form an MYF5, MYOD, MRF4 or combination thereof null non-human blastocyst; c) introducing human stem cells into the MYF5, MYOD, MRF4 or combination null non-human blastocyst of b); and d) implanting said blastocyst from c) into a pseudopregnant surrogate non-human animal to generate a chimeric non-human animal expressing human MYF5, MYOD, MRF4 or combination thereof.

公开(公告)号：US20240284884A1

公开(公告)日：2024-08-29

申请号：US18640282

申请日：2024-04-19

申请人： The Curators of the University of Missouri

发明人： Randall S. Prather ,  Kevin D. Wells ,  Kristin M. Whitworth

IPC分类号： A01K67/0276 ,  C07K14/705 ,  C12N15/85

CPC分类号： A01K67/0276 ,  C12N15/8509 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/108 ,  A01K2267/02 ,  C07K14/7056 ,  C07K14/70596

摘要： The present invention generally relates to genetically modified swine wherein at least one allele of a SIGLEC1 gene has been inactivated and/or at least one allele of a CD163 gene has been inactivated. Genetically modified swine having both alleles of the SIGLEC1 gene and/or both alleles CD163 gene inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSV). Methods for producing such transgenic swine are also provided.

公开(公告)号：US12048296B2

公开(公告)日：2024-07-30

申请号：US16972803

申请日：2019-06-07

申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

发明人： Yong Sam Kim ,  Jeong Heon Ko ,  Nan Ee Lee ,  Sun Hee Kim

IPC分类号： A01K67/0276 ,  C07K16/00 ,  C12N9/22 ,  C12N15/11 ,  G01N33/68

CPC分类号： A01K67/0276 ,  C07K16/00 ,  C12N9/22 ,  C12N15/11 ,  G01N33/68 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  C07K2317/41 ,  C12N2310/20 ,  C12N2800/80

摘要： The present disclosure relates to an aglycosylated antibody-producing transgenic mouse and the use of an aglycosylated antibody produced therefrom. When the transgenic mouse of the present disclosure is used, it is possible to easily produce aglycosylated antibodies against various target antigens, and to precisely diagnose disease by detecting a glycoprotein biomarker using the produced aglycosylated antibody.

公开(公告)号：US12037596B2

公开(公告)日：2024-07-16

申请号：US17196408

申请日：2021-03-09

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Jeffrey D. Lee ,  Alexander O. Mujica ,  Wojtek Auerbach ,  Ka-Man Venus Lai ,  David M. Valenzuela ,  George D. Yancopoulos

IPC分类号： A01K67/0278 ,  A01K67/0276 ,  A61D19/04 ,  C07K14/715 ,  C07K14/775 ,  C12N15/85 ,  C12N15/90

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K67/0278 ,  A61D19/04 ,  C07K14/7155 ,  C07K14/775 ,  C12N15/907 ,  A01K2217/07 ,  A01K2227/105 ,  A01K2267/0362 ,  A01K2267/0381 ,  C12N2015/8527 ,  C12N2800/30 ,  C12N2810/00

摘要： Compositions and methods are provided for modifying a rat genomic locus of interest using a large targeting vector (LTVEC) comprising various endogenous or exogenous nucleic acid sequences as described herein. Compositions and methods for generating a genetically modified rat comprising one or more targeted genetic modifications in their germline are also provided. Compositions and methods are provided which comprise a genetically modified rat or rat cell comprising a targeted genetic modification in the rat interleukin-2 receptor gamma locus, the rat ApoE locus, the rat Rag2 locus, the rat Rag1 locus and/or the rat Rag2/Rag1 locus. The various methods and compositions provided herein allows for these modified loci to be transmitted through the germline.

公开(公告)号：US20240196872A1

公开(公告)日：2024-06-20

申请号：US18101810

申请日：2023-01-26

申请人： Indiana University Research and Technology Corp

发明人： A. Joseph Tector

IPC分类号： A01K67/0276 ,  A61K35/407 ,  C12N9/02 ,  C12N9/10 ,  C12N9/22 ,  C12N9/40 ,  C12N15/85 ,  C12N15/877 ,  C12N15/90

CPC分类号： A01K67/0276 ,  A61K35/407 ,  C12N9/0071 ,  C12N9/1048 ,  C12N9/22 ,  C12N9/2465 ,  C12N15/8509 ,  C12N15/8778 ,  C12N15/907 ,  A01K2217/00 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/108 ,  A01K2267/025 ,  C07K2319/80

摘要： The invention provides double knockout transgenic pigs (GT/CMAH-KO pigs) lacking expression of any functional αGAL and CMAH. Double knockout GT/CMAH-KO transgenic organs, tissues and cells are also provided. Methods of making and using the GT/CMAH-KO pigs and tissue are also provided.

公开(公告)号：US12012437B2

公开(公告)日：2024-06-18

申请号：US17484324

申请日：2021-09-24

申请人： The Regents of the University of California

发明人： Stephanie Cherqui

IPC分类号： C07K14/47 ,  A01K67/0276 ,  A01K67/0278 ,  A61K35/28 ,  A61P25/28 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86

CPC分类号： C07K14/47 ,  A01K67/0276 ,  A01K67/0278 ,  A61K35/28 ,  A61P25/28 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  A01K2207/15 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/0318 ,  C07K2319/60 ,  C12N2310/20 ,  C12N2740/16043 ,  C12N2800/80

摘要： Provided herein are methods for treating a disease or disorder associated with mitochondrial dysfunction through ex vivo introduction of a nucleic acid molecule into hematopoietic stem and progenitor cells (HSPCs) followed by transplantation of the HSPCs into a subject in need of treatment. The nucleic acid molecule may include a functional human frataxin (hFXN) or may include a gene editing system that when transfected into the cells removes a trinucleotide extension mutation of endogenous hFXN.

公开(公告)号：US12012436B2

公开(公告)日：2024-06-18

申请号：US16082487

申请日：2017-03-15

申请人： The Regents of the University of California

发明人： Stephanie Cherqui

IPC分类号： C07K14/47 ,  A01K67/0276 ,  A01K67/0278 ,  A61K35/28 ,  A61P25/28 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86

CPC分类号： C07K14/47 ,  A01K67/0276 ,  A01K67/0278 ,  A61K35/28 ,  A61P25/28 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  A01K2207/15 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/0318 ,  C07K2319/60 ,  C12N2310/20 ,  C12N2740/16043 ,  C12N2800/80

摘要： Provided herein are methods for treating a disease or disorder associated with mitochondrial dysfunction through ex vivo introduction of a nucleic acid molecule into hematopoietic stem and progenitor cells (HSPCs) followed by transplantation of the HSPCs into a subject in need of treatment. The nucleic acid molecule may include a functional human frataxin (hFXN) or may include a gene editing system that when transfected into the cells removes a trinucleotide extension mutation of endogenous hFXN.

公开(公告)号：US12102069B2

公开(公告)日：2024-10-01

申请号：US16825858

申请日：2020-03-20

申请人： Washington State University ,  University of Maryland ,  The University Court of The University of Edinburgh

发明人： Jon Michael Oatley ,  Christopher Bruce Alexander Whitelaw ,  Simon Geoffrey Lillico ,  Bhanu Prakash Telugu

IPC分类号： A01K67/00 ,  A01K67/0276 ,  C12N9/22 ,  C12N15/90 ,  G01N33/00

CPC分类号： A01K67/0276 ,  C12N9/22 ,  C12N15/907 ,  C12Y301/00 ,  A01K2217/075 ,  A01K2227/101 ,  A01K2227/108 ,  A01K2267/025

摘要： The present invention provides livestock animals and methods to create recipient animals for spermatogonial stem cell transplantation through modulation of the NANOS gene. In one embodiment genome editing issued to create animals with insertions or deletions (indels) that inactivate or otherwise modulate NANOS gene activity so that resulting males lack functional germ cells yet retain functional somatic cells, and females are fertile. These males can then be transplanted with donor spermatogonial stem cells and used for breeding.

公开(公告)号：US12070022B2

公开(公告)日：2024-08-27

申请号：US17379898

申请日：2021-07-19

申请人： Recombinetics, Inc.

发明人： Scott C. Fahrenkrug ,  Daniel F. Carlson

IPC分类号： C12N15/63 ,  A01K67/0275 ,  A01K67/0276 ,  C12N9/22 ,  C12N15/79 ,  C12N15/873 ,  C12N15/90

CPC分类号： A01K67/0276 ,  A01K67/0275 ,  C12N9/22 ,  C12N15/873 ,  C12N15/907 ,  A01K2217/07 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/101 ,  A01K2227/108 ,  A01K2267/02

摘要： The present disclosure relates to methods for making genetic edits in vitro in a non-human vertebrate cell or embryo at a plurality of target chromosomal DNA sites. Methods for making a non-human animal having multiplex genetic edits at a plurality of target chromosomal DNA sites and making a non-human vertebrate animal chimeric for host cells and donor cells are also considered.

公开(公告)号：US12036288B2

公开(公告)日：2024-07-16

申请号：US17241763

申请日：2021-04-27

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Lynn MacDonald ,  Naxin Tu ,  Cagan Gurer ,  Sean Stevens ,  Andrew J. Murphy

IPC分类号： G01N33/00 ,  A01K67/0276 ,  A01K67/0278 ,  A61K49/00 ,  C07K14/735 ,  G01N33/50 ,  A01K67/027

CPC分类号： A61K49/0008 ,  A01K67/0276 ,  A01K67/0278 ,  C07K14/70535 ,  G01N33/5014 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0387

摘要： Genetically modified non-human animals and methods and compositions for making and using them are provided, wherein the genetic modification comprises a deletion of the endogenous low affinity FcγR locus, and wherein the mouse is capable of expressing a functional FcRγ-chain. Genetically modified mice are described, including mice that express low affinity human FcγR genes from the endogenous FcγR locus, and wherein the mice comprise a functional FcRγ-chain. Genetically modified mice that express up to five low affinity human FcγR genes on accessory cells of the host immune system are provided.