公开(公告)号：US5294533A

公开(公告)日：1994-03-15

申请号：US572191

申请日：1990-08-23

申请人： James R. Lupski ,  Leonard Katz

发明人： James R. Lupski ,  Leonard Katz

IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61P31/04 ,  C07H21/04 ,  C07K14/195 ,  C07K14/285 ,  C12N15/113 ,  C12N15/31 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/68 ,  C12R1/125 ,  C12R1/19 ,  C12R1/42 ,  C21Q1/68 ,  C12N15/00 ,  C12N15/11

CPC分类号： C12Q1/18 ,  C07K14/195 ,  C07K14/285 ,  C12N15/113 ,  A61K38/00 ,  C12N2310/3517

摘要： A method of interrupting the expression of a macromolecular synthesis operon in bacteria comprising the step of binding an antisense oligonucleotide to a single stranded DNA or to a mRNA transcribed from the macromolecular synthesis operon. The antisense oligonucleotide can be either sequence specific to a unique intergenic sequence or a sequence specific to a bacterial homologous sequence. By interrupting the expression of the macromolecular synthesis operon bacterial infections can be treated. Specific antisense oligonucleotides are disclosed. The ability of the antisense oligonucleotide to bind the mRNA or single stranded DNA also allows the identification of the bacteria by using a unique intergenic antisense oligonucleotide to bind to the single stranded DNA or to the mRNA transcribed from the macromolecular synthesis operon. A method for competitively inhibiting the protein products of the MMS operon with oligonucleotides is also disclosed. Methods of identifying unique intergenic sequence is also disclosed.

摘要翻译： 一种中断细菌中大分子合成操纵子表达的方法，包括将反义寡核苷酸与单链DNA或从大分子合成操纵子转录的mRNA结合的步骤。 反义寡核苷酸可以是独特的基因间序列特异性序列或对细菌同源序列特异性的序列。 通过中断大分子合成操纵子的表达可以治疗细菌感染。 公开了具体的反义寡核苷酸。 反义寡核苷酸结合mRNA或单链DNA的能力还允许通过使用独特的基因间反义寡核苷酸结合单链DNA或从大分子合成操纵子转录的mRNA来鉴定细菌。 还公开了用寡核苷酸竞争性抑制MMS操纵子的蛋白质产物的方法。 还公开了识别独特的基因间序列的方法。

公开(公告)号：US07163658B2

公开(公告)日：2007-01-16

申请号：US10421343

申请日：2003-04-23

申请人： Rouvain Bension

发明人： Rouvain Bension

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  C21Q1/68

CPC分类号： C12Q1/6869 ,  C12Q2565/601 ,  C12Q2565/519 ,  C12Q2523/307

摘要： A method and device for sequencing at least a fragment of a linear polymer. The device comprises a well for placement of a rotaxane comprising the combination of a cyclic molecule and a linear polymer threaded through said cyclic molecule; a probe having the ability to move the linear polymer relative to the cyclic molecule while producing a signal resulting from the interaction of the cyclic molecule and a unit attached to the polymer; and means for reading said signal. The process comprises formation of the rotaxane, attachment of the probe, movement of the cyclic molecule relative to the linear polymer and the reading of signals. The device and method are especially useful for the sequencing of DNA.

摘要翻译： 用于对至少一个线性聚合物的片段进行测序的方法和装置。 该装置包括用于放置轮烷的孔，该轮烷包含环状分子和穿过所述环状分子的线性聚合物的组合; 探针具有相对于环状分子移动线性聚合物的能力，同时产生由环状分子与连接于聚合物的单元相互作用而产生的信号; 以及用于读取所述信号的装置。 该方法包括形成轮烷，探针的附着，环状分子相对于线性聚合物的移动和信号的读取。 该装置和方法对DNA的测序特别有用。

公开(公告)号：US06936419B1

公开(公告)日：2005-08-30

申请号：US10148140

申请日：2000-11-24

申请人： Kurt Berlin

发明人： Kurt Berlin

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07H21/04 ,  C21Q1/68

CPC分类号： C12Q1/6827 ,  C12Q1/6837 ,  C12Q2523/125

摘要： An oligomer array with PNA (peptide nucleic acid) and/or DNA oligomers on a surface is described, which comprises oligomers of between 6 and 20 monomers or nucleobases each, whereby each of these contains at least one sequence of the general formula DDCGDD or of the general formula DDTGDD or of the general formula HHCGHH or of the general formula HHCAHH, wherein H indicates one of the bases: adenine (A), cytosine (C), or thymine (T) and D represents one of the bases: adenine (A), guanine (G) or thymine (T), and wherein the site of the oligomers on the surface is correlated with the sequence of the oligomers. The oligomer arrays according to the invention are used for the detection of cytosine methylations in genomic DNA.

摘要翻译： 描述了在表面上具有PNA（肽核酸）和/或DNA寡聚体的低聚物阵列，其包含6至20个单体或核碱基的寡聚物，其中每个含有至少一个通式DDCGDD的序列或 通式DDTGDD或通式为HHCGHH或通式HHCAHH，其中H表示碱基之一：腺嘌呤（A），胞嘧啶（C）或胸腺嘧啶（T），D表示碱基之一：腺嘌呤（ A），鸟嘌呤（G）或胸腺嘧啶（T），并且其中表面上的寡聚物的位点与寡聚体的序列相关。 根据本发明的寡聚体阵列用于检测基因组DNA中的胞嘧啶甲基化。

公开(公告)号：US07413859B2

公开(公告)日：2008-08-19

申请号：US10841413

申请日：2004-05-07

申请人： Christian Paulus ,  Petra T. Schindler-Bauer

发明人： Christian Paulus ,  Petra T. Schindler-Bauer

IPC分类号： C21Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： G01N33/5438

摘要： Method for detecting macromolecular biopolymers using a unit for immobilizing macromolecular biopolymers, in which the unit is provided with first molecules serving as capture molecules. The method includes the steps of bringing a sample into contact with the unit, it being possible for the sample to contain the macromolecular biopolymers, and the macromolecular biopolymers or the first molecules having a marking which is used to generate a detectable signal, binding macromolecular biopolymers contained in the sample to the capture molecules, thereby forming complexes comprising capture molecules and macromolecular biopolymers, exciting the emission of a signal by means of the marking, detecting the signal emitted by means of the marking, separating the complexes comprising capture molecules and macromolecular biopolymers, thereby altering the intensity of the emitted signal, and detecting the separation of the complexes comprising capture molecules and macromolecular biopolymers by means of the change in the intensity of the signal.

摘要翻译： 使用用于固定大分子生物聚合物的单元检测大分子生物聚合物的方法，其中该单元具有用作捕获分子的第一分子。 该方法包括使样品与单元接触的步骤，样品可以含有大分子生物聚合物，大分子生物聚合物或具有用于产生可检测信号的标记的第一分子，结合大分子生物聚合物 包含在捕获分子中的样品，从而形成包含捕获分子和大分子生物聚合物的复合物，通过标记激发信号的发射，检测通过标记发射的信号，分离包含捕获分子和大分子生物聚合物的复合物 从而改变发射信号的强度，并通过信号强度的变化来检测包含捕获分子和大分子生物聚合物的络合物的分离。

公开(公告)号：US07189508B2

公开(公告)日：2007-03-13

申请号：US10197026

申请日：2002-07-17

申请人： Joseph Sorge ,  Anne M. Whalen

发明人： Joseph Sorge ,  Anne M. Whalen

IPC分类号： C21Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6823 ,  C12Q2537/1373 ,  C12Q2537/137 ,  C12Q2565/1015 ,  C12Q2537/1376

摘要： The invention relates to generating a signal indicative of a target nucleic acid sequence, comprising forming a complex by incubating a sample comprising a target nucleic acid sequence with a probe comprising a first and second subunit, and a binding moiety, and dissociating the first and second subunit to release the first subunit and generate a signal. The invention also relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising forming a complex by incubating a target nucleic acid sequence, an upstream primer and a probe comprising a first and second subunit, and a binding moiety. The primer is extended with a nucleic acid polymerase to displace a portion of the first subunit from the target nucleic acid strand thereby dissociating the first subunit from the second subunit to release the first subunit and generate a signal.

摘要翻译： 本发明涉及产生指示靶核酸序列的信号，包括通过将包含靶核酸序列的样品与包含第一和第二亚基的探针和结合部分一起孵育而形成复合物，并解离第一和第二 子单元释放第一个子单元并产生信号。 本发明还涉及产生指示样品中靶核酸序列存在的信号的方法，包括通过孵育靶核酸序列，上游引物和包含第一和第二亚基的探针形成复合物， 和结合部分。 用核酸聚合酶扩增引物以从目标核酸链取代部分第一亚基，从而将第一亚基与第二亚基解离以释放第一亚基并产生信号。

公开(公告)号：US6054279A

公开(公告)日：2000-04-25

申请号：US120916

申请日：1998-07-20

申请人： James G. Nadeau ,  J. Bruce Pitner ,  James L. Schram ,  C. Preston Linn ,  Glenn P. Vonk ,  G. Terrance Walker

发明人： James G. Nadeau ,  J. Bruce Pitner ,  James L. Schram ,  C. Preston Linn ,  Glenn P. Vonk ,  G. Terrance Walker

IPC分类号： G01N21/64 ,  C07H21/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/58 ,  C21Q1/68

CPC分类号： C12Q1/6818 ,  C12Q1/6825

摘要： Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.

摘要翻译： 通过连接到形成供体/受体染料对的两种染料来修饰单链信号引物。 这两种染料位于信号引物足够接近的空间上，第一种染料的荧光被第二种染料淬灭。 信号引物还可以包含两种染料之间的限制性内切核酸酶识别位点（RERS）。 由于信号引物最初是单链的，并且在不存在靶标的情况下保持单链，限制性内切核酸酶识别位点不受限制性内切核酸酶的切割或切割。 然而，在靶的存在下，信号引物和限制性内切核酸酶识别位点被限制性内切核酸酶双链和切割或切割。 切割或切口分离两种染料，并且由于猝灭降低引起的荧光变化被检测为目标序列或靶序列扩增的存在的指示。