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18 条记录 (耗时 0.03 秒)

    Antisense oligonucleotide antibiotics complementary to the macromolecular synthesis operon, methods of treating bacterial infections and methods for identification of bacteria
    1.
    发明授权
    Antisense oligonucleotide antibiotics complementary to the macromolecular synthesis operon, methods of treating bacterial infections and methods for identification of bacteria 失效
    与大分子合成操纵子互补的反义寡核苷酸抗生素，细菌感染的治疗方法和细菌鉴定方法

    公开(公告)号：US5294533A

    公开(公告)日：1994-03-15

    申请号：US572191

    申请日：1990-08-23

    申请人： James R. Lupski ,  Leonard Katz

    发明人： James R. Lupski ,  Leonard Katz

    IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61P31/04 ,  C07H21/04 ,  C07K14/195 ,  C07K14/285 ,  C12N15/113 ,  C12N15/31 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/68 ,  C12R1/125 ,  C12R1/19 ,  C12R1/42 ,  C21Q1/68 ,  C12N15/00 ,  C12N15/11

    CPC分类号： C12Q1/18 ,  C07K14/195 ,  C07K14/285 ,  C12N15/113 ,  A61K38/00 ,  C12N2310/3517

    摘要： A method of interrupting the expression of a macromolecular synthesis operon in bacteria comprising the step of binding an antisense oligonucleotide to a single stranded DNA or to a mRNA transcribed from the macromolecular synthesis operon. The antisense oligonucleotide can be either sequence specific to a unique intergenic sequence or a sequence specific to a bacterial homologous sequence. By interrupting the expression of the macromolecular synthesis operon bacterial infections can be treated. Specific antisense oligonucleotides are disclosed. The ability of the antisense oligonucleotide to bind the mRNA or single stranded DNA also allows the identification of the bacteria by using a unique intergenic antisense oligonucleotide to bind to the single stranded DNA or to the mRNA transcribed from the macromolecular synthesis operon. A method for competitively inhibiting the protein products of the MMS operon with oligonucleotides is also disclosed. Methods of identifying unique intergenic sequence is also disclosed.

    摘要翻译： 一种中断细菌中大分子合成操纵子表达的方法，包括将反义寡核苷酸与单链DNA或从大分子合成操纵子转录的mRNA结合的步骤。 反义寡核苷酸可以是独特的基因间序列特异性序列或对细菌同源序列特异性的序列。 通过中断大分子合成操纵子的表达可以治疗细菌感染。 公开了具体的反义寡核苷酸。 反义寡核苷酸结合mRNA或单链DNA的能力还允许通过使用独特的基因间反义寡核苷酸结合单链DNA或从大分子合成操纵子转录的mRNA来鉴定细菌。 还公开了用寡核苷酸竞争性抑制MMS操纵子的蛋白质产物的方法。 还公开了识别独特的基因间序列的方法。

    Fingerprinting bacterial strains using repetitive DNA sequence amplification
    6.
    发明授权
    Fingerprinting bacterial strains using repetitive DNA sequence amplification 失效
    使用重复DNA序列扩增的指纹细菌菌株

    公开(公告)号：US5691136A

    公开(公告)日：1997-11-25

    申请号：US111077

    申请日：1993-08-24

    申请人： James R. Lupski ,  James Versalovic ,  Thearith Koeuth

    发明人： James R. Lupski ,  James Versalovic ,  Thearith Koeuth

    IPC分类号： C12N1/11 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6876 ,  C12Q1/689 ,  C12Q2600/16

    摘要： Oligonucleotide primers and methods for identifying strains of bacteria by genomic fingerprinting are described. The methods are applicable to a variety of samples. The testing procedure includes amplifying the bacterial DNA in the sample to be tested by adding a pair of outwardly-directed primers to the sample. The primers are capable of hybridizing to repetitive DNA sequences in the bacterial DNA and extending outwardly from one hybridizable repetitive sequence to another hybridizable repetitive sequence. After amplification the extension products are separated by size and the specific strain of bacteria is determined by measuring the pattern of sized extension products. The procedure to identify strains of bacteria by fingerprinting has a variety of uses including: identifying bacteria in infections, agriculture and horticulture plots, bioremediation, food monitoring, production monitoring and quality assurance and quality control.

    摘要翻译： 描述了寡核苷酸引物和通过基因组指纹识别细菌菌株的方法。 该方法适用于各种样品。 测试方法包括通过向样品中加入一对向外引物来扩增待测样品中的细菌DNA。 引物能够与细菌DNA中的重复DNA序列杂交并从一个可杂交的重复序列向外延伸到另一个可杂交的重复序列。 扩增后，延伸产物按大小分离，细菌的特异菌株通过测量大小的延伸产物的图案来确定。 通过指纹识别细菌菌株的方法有多种用途，包括：鉴定感染，农业和园艺地块中的细菌，生物修复，食品监测，生产监测和质量保证以及质量控制。

    Defects in periaxin associated with myelinopathies
    7.
    发明授权
    Defects in periaxin associated with myelinopathies 有权
    与myelinopathies相关的periaxin缺陷

    公开(公告)号：US07273698B2

    公开(公告)日：2007-09-25

    申请号：US10021955

    申请日：2001-12-13

    申请人： James R. Lupski ,  Cornelius F. Boerkoel, III ,  Hiroshi Takashima

    发明人： James R. Lupski ,  Cornelius F. Boerkoel, III ,  Hiroshi Takashima

    IPC分类号： C12Q1/68 ,  C07H21/02

    CPC分类号： C12Q1/6883 ,  C07K14/47 ,  C12Q2600/156 ,  G01N33/6893 ,  G01N2800/285

    摘要： The present invention relates to defects in periaxin (PRX) associated with myelinopathies, including Charcot-Marie-Tooth syndrome and/or Dejerine-Sottas syndrome. Unrelated individuals having a myelinopathy from Dejerine-Sottas syndrome have recessive PRX mutations. The PRX locus maps to a region associated with a severe autosomal recessive demyelinating neuropathy and is also syntenic to the Prx location on murine chromosome 7.

    摘要翻译： 本发明涉及与髓鞘病相关的periaxin（PRX）缺陷，包括Charcot-Marie-Tooth综合征和/或Dejerine-Sottas综合征。 不相关的患有Dejerine-Sottas综合征的髓鞘病的个体具有隐性PRX突变。 PRX位点映射到与严重常染色体隐性脱髓鞘神经病相关的区域，并且也与鼠染色体7上的Prx位点相同。