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    METHODS AND COMPOSITIONS FOR INTERNALLY CONTROLLED IN SITU ASSAYS
    2.
    发明申请
    METHODS AND COMPOSITIONS FOR INTERNALLY CONTROLLED IN SITU ASSAYS 有权

    公开(公告)号：US20250034624A1

    公开(公告)日：2025-01-30

    申请号：US18763973

    申请日：2024-07-03

    Applicant: 10x Genomics, Inc.

    Inventor： Felice Alessio BAVA

    IPC: C12Q1/6827 ,  C12Q1/6841 ,  C12Q1/6853 ,  C12Q1/6876

    Abstract: The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, the methods involve the use of a set of polynucleotides, including one or more polynucleotides (e.g., a detection padlock probe) for detecting a region of interest and one or more polynucleotides (e.g., a control padlock probe) as an internal control, for analyzing target nucleic acids. In some aspects, the presence, amount, and/or identity of a region of interest in a target nucleic acid is analyzed in situ. Also provided are polynucleotides, sets of polynucleotides, compositions, and kits for use in accordance with the methods.

    METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING
    4.
    发明申请
    METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING 有权

    公开(公告)号：US20250019761A1

    公开(公告)日：2025-01-16

    申请号：US18648154

    申请日：2024-04-26

    Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

    Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

    IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

    Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

    COMPOSITIONS AND METHODS FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS
    8.
    发明申请
    COMPOSITIONS AND METHODS FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS 有权

    公开(公告)号：US20240425940A1

    公开(公告)日：2024-12-26

    申请号：US18757285

    申请日：2024-06-27

    Applicant: Cue Health Inc.

    Inventor： Ayub Khattak

    IPC: C12Q1/70 ,  C12Q1/6844 ,  C12Q1/6876

    Abstract: The present disclosure relates generally to recombinant nucleic acid primers (oligonucleotide) comprising a hairpin structure and a methylated RNA region, and nucleic acid amplification processes comprising the recombinant nucleic acid primers for the isothermal amplification of target nucleic acids with higher specificity and sensitivity in single or multiplexed assays. Further provided are compositions comprising the recombinant nucleic acid primers, and devices suitable for use in or with the nucleic acid amplification processes.

    SYSTEMS AND METHODS FOR PROTEIN DETECTION
    9.
    发明申请
    SYSTEMS AND METHODS FOR PROTEIN DETECTION 有权

    公开(公告)号：US20240425921A1

    公开(公告)日：2024-12-26

    申请号：US18787313

    申请日：2024-07-29

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  ONE LAMBDA, INC.

    Inventor： Rui PEI ,  Peter VANDER HORN ,  Hong MA ,  Ito WAKEFIELD

    IPC: C12Q1/6876 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6844

    Abstract: Methods, systems, and devices for analyzing a sample for the presence of a target protein are provided. A sample can be processed, the processing generating an amplification product of a template nucleic acid formed in response to a target protein being present in the sample. The amplification product can be contacted with a detection probe to generate a second product comprising a labeled nucleic acid. A lateral flow substrate can be contacted with the second product, the lateral flow substrate comprising a capture moiety configured to bind the labeled nucleic acid. The methods can be carried out in one or more chambers of a single device or multiple devices. Detection probe bound to amplicons of the template nucleic acid captured on the lateral flow substrate can be read visually or using a sensor.

    EPITRANSCRIPTOME EVALUATION
    10.
    发明申请
    EPITRANSCRIPTOME EVALUATION 有权

    公开(公告)号：US20240425914A1

    公开(公告)日：2024-12-26

    申请号：US18687476

    申请日：2022-08-29

    Applicant: HTG Molecular Diagnostics, Inc.

    Inventor： Debrah Thompson

    IPC: C12Q1/6869 ,  C12Q1/6804 ,  C12Q1/6876

    Abstract: The present disclosure provides methods of using quantitative nuclease protection sequencing (qNPS) methods to evaluate epitranscriptomes. The disclosed methods capture nucleic acid molecules that are modified as a result of exposure to an agent, such as stress, a mutagen, a microbe (such as a pathogen), or therapeutic agent. The resulting modified nucleic acid molecules are then directly or indirectly sequenced using nuclease protection probes (NPPs) and qNPS, allowing for the detection of target and off-target modifications of an epitranscriptome.

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