公开(公告)号：US12011459B2

公开(公告)日：2024-06-18

申请号：US16893246

申请日：2020-06-04

申请人： Immatics US, Inc. ,  Immatics Biotechnologies GmbH

发明人： Amir Alpert ,  Dominik Maurer ,  Anastasiya Smith ,  Claudia Wagner ,  Ali Mohamed

IPC分类号： A61K35/17 ,  A61K31/437 ,  A61K31/5383 ,  A61K35/76 ,  C07K14/47 ,  C07K14/725 ,  C12N5/0783 ,  G01N15/14 ,  G01N33/554 ,  G01N15/10 ,  G01N15/149

CPC分类号： A61K35/17 ,  A61K31/437 ,  A61K31/5383 ,  A61K35/76 ,  C07K14/47 ,  C07K14/7051 ,  C12N5/0636 ,  G01N15/1459 ,  G01N33/554 ,  C12N2501/2307 ,  C12N2501/50 ,  C12N2523/00 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N15/149 ,  G01N2333/705 ,  G01N2333/7051 ,  G01N2500/10

摘要： Described herein are methods for preparing T cells, including isolating CD8+ T cells from a blood sample obtained from a patient or a donor, culturing the isolated CD8+ T cells in the presence of at least one cytokine, contacting the cultured CD8+ T cells with a multimer containing a target peptide in a complex with an MHC molecule and with at least one binding agent that binds to a T cell surface molecule, in which the multimer is labelled with a first detectable agent and the binding agent is labelled with a second detectable agent, sorting the contacted CD8+ T cells to collect the sorted CD8+ T cells that are detected positive for the first and the second detectable agents, and expanding the collected CD8+ T cells.

公开(公告)号：US11668713B2

公开(公告)日：2023-06-06

申请号：US16347502

申请日：2017-11-16

申请人： University of Washington

发明人： Daniel T. Chiu ,  Chun-Ting Kuo ,  Li Wu

IPC分类号： G01N33/554 ,  G01N33/533 ,  B01L3/00 ,  G01N15/14 ,  G01N21/64

CPC分类号： G01N33/554 ,  B01L3/502715 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/533 ,  B01L2300/047 ,  B01L2300/06 ,  B01L2300/0627 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2300/18 ,  G01N2021/6432

摘要： Methods and systems for improved labeling and/or de-labeling a molecule or cell in the context of scientific experimentation, industrial applications, and clinical investigation, including the means to repeat the process of labeling and de-labeling in an efficient manner.

公开(公告)号：US20230029386A1

公开(公告)日：2023-01-26

申请号：US17932600

申请日：2022-09-15

申请人： EMORY UNIVERSITY ,  ONE LAMBDA, INC.

发明人： Robert A. Bray ,  Jar-How Lee

IPC分类号： C12Q1/68 ,  G01N33/554 ,  C07K14/74 ,  G01N33/68 ,  C12N15/85

摘要： Described herein are materials and methods of incorporating CLIP peptide into the peptide binding groove of HLA Class II antigens and using such HLA Class II antigens for the detection of alloantibodies.

公开(公告)号：US11428692B2

公开(公告)日：2022-08-30

申请号：US16033064

申请日：2018-07-11

申请人： VISIONGATE, INC.

发明人： Daniel J. Sussman ,  Timothy Bell ,  Frances Ginn Howard ,  Jonus Reyna

IPC分类号： G01N33/533 ,  G01N33/574 ,  G01N21/64 ,  G01N33/554

摘要： A method for enhancing gating performance of a cell sorter to prepare an enriched specimen for optical tomography cell analysis includes introducing a specimen into a FACS to generate 2D event data; generating a first scatterplot of the 2D data; identifying target objects; constructing a boundary within the first scatterplot to produce a first gate; counting target objects within the first gate; comparing the number of target objects within the first gate to a first predetermined value and adjusting the first gate as necessary. A boundary around a set of target objects is constructed in a second scatterplot to produce a subset second gate and target objects within the second gate are counted and the count compared to a second predetermined value. When a boundary around target objects meets specifications the first and second gates are stored in memory and used to enrich patient specimens.

公开(公告)号：US20220267827A1

公开(公告)日：2022-08-25

申请号：US17498627

申请日：2021-10-11

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Garry P. Nolan ,  Nikolay Samusik ,  Julia Kennedy-Darling ,  Yury Goltsev

IPC分类号： C12Q1/6804 ,  C12Q1/6813 ,  G01N33/53 ,  G01N1/30 ,  G01N33/543 ,  G01N33/554

摘要： Provided herein is a method and system for analyzing a sample. In some embodiments the method makes use of a plurality of capture agents that are each linked to a different oligonucleotide and a corresponding plurality of labeled nucleic acid probes, wherein each of the labeled nucleic acid probes specifically hybridizes with only one of the oligonucleotides. The sample is labeled with the capture agents en masse, and sub-sets of the capture agents are detected using iterative cycles using corresponding subsets of the labeled nucleic acid probes.

公开(公告)号：US10852300B2

公开(公告)日：2020-12-01

申请号：US15578493

申请日：2016-06-01

申请人： TANAKA KIKINZOKU KOGYO K.K.

发明人： Keita Suzuki ,  Hisahiko Iwamoto

IPC分类号： G01N33/554 ,  G01N33/569 ,  C07K14/30 ,  G01N33/543 ,  G01N33/531

摘要： The present invention is intended to provide an immunochromatographic analyzer that enables a quick and easy, high-sensitivity detection of Mycoplasma pneumoniae, and thus more reliable and faster diagnosis of mycoplasma pneumonia. The immunochromatographic analyzer according to the present invention is for detecting Mycoplasma pneumoniae, and includes a sample adding section, a label-substance retaining section, a chromatographic medium section having a detection section, and an absorbing section. The label-substance retaining section and the detection section contain an antibody that strongly recognizes domain III of P30 protein of Mycoplasma pneumoniae consisting of the amino acid sequence of SEQ ID NO: 2.

公开(公告)号：US10823719B2

公开(公告)日：2020-11-03

申请号：US15753303

申请日：2016-08-17

申请人： SWISS ANALYZE BACTERIA AG

发明人： Dieter Ebert ,  Mathias Reichl ,  Werner Lubitz

IPC分类号： G01N33/554 ,  G01N33/18 ,  G01N33/537 ,  G01N33/569 ,  G01N33/58 ,  G01N21/03 ,  G01N21/65

摘要： The invention relates to methods and devices for quantitative determination of bacteria.

公开(公告)号：US10456441B2

公开(公告)日：2019-10-29

申请号：US15892280

申请日：2018-02-08

申请人： The University of Vermont and State Agricultural College

发明人： Ralph C. Budd ,  Cheryl Collins

IPC分类号： G01N33/554 ,  C07K1/00 ,  A61K38/06 ,  A61K39/02 ,  A61K39/39 ,  A61K35/15 ,  A61K39/00 ,  A61K9/51

摘要： Methods and products for producing an antigen specific immune response are provided. The methods involve administration of a caspase inhibitor to a subject.

公开(公告)号：US20190264194A1

公开(公告)日：2019-08-29

申请号：US16005583

申请日：2018-06-11

申请人： The Regents of the University of California

发明人： Patrick S. Daugherty ,  Paul H. Bessette ,  Jeffrey Rice

IPC分类号： C12N15/10 ,  C07K14/245 ,  C40B40/10 ,  G01N33/554 ,  C40B30/04

摘要： Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed.

公开(公告)号：US10351970B2

公开(公告)日：2019-07-16

申请号：US15055814

申请日：2016-02-29

申请人： BioInvent International AB

发明人： Björn Frendéus

IPC分类号： G01N33/68 ,  G01N33/554 ,  C12N15/10 ,  C40B30/04

摘要： The present invention relates to screening methods and, in particular, to methods of screening anti-ligand libraries for identifying anti-ligands specific for differentially and/or infrequently expressed ligands. The method comprises the steps of providing a library of anti-ligands: providing a first subtractor ligand; providing a second target ligand; determining the amount of the first and second target ligands using one or more equations derived from the universal law of mass action; providing the determined amount of a first subtractor ligand; providing the determined amount of a second target ligand; providing separation means capable of use to isolate anti-ligand bound to the second target ligand from anti-ligand bound to the first subtractor ligand; exposing the library of to the first and second target ligands to permit binding of anti-ligands to ligands; and using the separation means to isolate the anti-ligand bound to second target ligand.