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公开(公告)号：US20230159979A1
公开(公告)日：2023-05-25
申请号：US17904916
申请日：2021-04-15
发明人： Daniel T. Chiu , Haobin Chen , Jiangbo Yu
CPC分类号： C12Q1/32 , C12Q1/008 , C09K11/06 , C09K11/025 , B82Y20/00
摘要： Transducers, kits, systems, and methods for determining a concentration of an analyte are described. In an embodiment, the transducers include a chromophore; and an enzyme physically associated with the chromophore. In an embodiment, the transducer is configured to catalyze a reaction comprising a plurality of reaction elements. In an embodiment, the plurality of reaction elements comprises one or more reactants including the analyte and one or more products. In an embodiment, an amount of fluorescence emitted from the chromophore is determined by a concentration of a reaction element of the plurality of reaction elements.
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公开(公告)号：US11648560B2
公开(公告)日：2023-05-16
申请号：US16639083
申请日：2018-08-15
发明人： Daniel T. Chiu , Mengxia Zhao , Eleanor S. Shah , Perry G. Schiro , Hui Min Yu , Wei-Feng Fang , Jui-Lin Chen
CPC分类号： B01L3/502761 , B01L3/502715 , B01L3/502738 , G01N15/1434 , G01N15/1459 , G01N33/4915 , B01L2200/0663 , B01L2300/06 , B01L2300/0681 , B01L2300/0864 , B01L2400/06 , B01L2400/084 , G01N15/1484 , G01N2015/1006 , G01N2015/149 , G01N2015/1486
摘要： Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and can be contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.
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公开(公告)号：US11480575B2
公开(公告)日：2022-10-25
申请号：US16702176
申请日：2019-12-03
发明人： Daniel T. Chiu , Mengxia Zhao , Wyatt Nelson , Perry G. Schiro
IPC分类号： G01N33/574 , B01L3/00 , G01N15/06 , G01N15/14 , G01N33/49 , G01N21/64 , G01N33/53 , G01N35/10 , G01N35/00 , G01N15/10
摘要： Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.
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公开(公告)号：US20220252607A1
公开(公告)日：2022-08-11
申请号：US17672586
申请日：2022-02-15
发明人： Daniel T. Chiu , Changfeng Wu , Jason McNeill , Jiangbo Yu
摘要： The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.
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公开(公告)号：US11408884B2
公开(公告)日：2022-08-09
申请号：US16702228
申请日：2019-12-03
发明人： Daniel T. Chiu , Wei Sun , Jiangbo Yu , Changfeng Wu , Fangmao Ye
IPC分类号： G01N33/58 , C09K11/06 , G01N33/543 , C09K11/02 , C08G61/02 , C08G73/00 , C08G75/32 , G01N33/533
摘要： Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.
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公开(公告)号：US20200011874A1
公开(公告)日：2020-01-09
申请号：US16556006
申请日：2019-08-29
发明人： Daniel T. Chiu , Changfeng Wu , Jiangbo Yu
摘要： The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.
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公开(公告)号：US10429294B2
公开(公告)日：2019-10-01
申请号：US15563121
申请日：2016-04-01
发明人： Daniel T. Chiu , Chun-Ting Kuo , Jiangbo Yu
IPC分类号： G01N33/58 , G01N21/64 , G01N21/25 , G01N15/14 , A61K49/00 , C12Q1/68 , C12N13/00 , G01N15/10
摘要： Methods of optically marking and sorting adherent cells are provided. The methods include providing a plurality of adherent cells attached to a substrate, each adherent cell of the plurality of adherent cells having an optical marker. The methods also include selectively applying light energy to a subset of the plurality of adherent cells, and detaching the plurality of adherent cells from the substrate. These methods also provide the sorting of the plurality of adherent cells.
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公开(公告)号：US10421070B2
公开(公告)日：2019-09-24
申请号：US14869871
申请日：2015-09-29
摘要： Embodiments of the present invention relate to methods and apparatuses for the discretization and manipulation of sample volumes that is simple, robust, and versatile. It is a fluidic device that partitions a sample by exploiting the interplay between fluidic forces, interfacial tension, channel geometry, and the final stability of the formed droplet and/or discretized volume. These compartmentalized volumes allow for isolation of samples and partitioning into a localized array that can subsequently be manipulated and analyzed. The isolation of the discretized volumes along with the device's inherent portability render our invention versatile for use in many areas, including but not limited to PCR, digital PCR, biological assays for diagnostics and prognostics, cancer diagnosis and prognosis, high throughput screening, single molecule and single cell reactions or assays, the study crystallization and other statistical processes, protein crystallization, drug screening, environmental testing, and the coupling to a wide range of analytical detection techniques for biomedical assays and measurements. The minimal fluid interconnects and simple flow geometry makes the device easy to use and implement, economical to fabricate and operate, and robust in its operations.
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公开(公告)号：US20190120857A1
公开(公告)日：2019-04-25
申请号：US16171918
申请日：2018-10-26
发明人： Daniel T. Chiu , Perry G. Schiro , Jason S. Kuo
IPC分类号： G01N33/68 , G01N33/574 , G01N33/53
摘要： Provided herein, among other aspects, are methods and apparatuses for ranking aliquots from a suspension containing bioparticles. In certain embodiments, the bioparticles may be cells, organelles, proteins, DNAs, debris of biological origin, microbeads coated with biological compounds, or viral particles. As such, the methods and apparatuses provided herein may be used to quantify rare cells such as circulating cancer cells, fetal cells and other rare cells present in bodily fluids for disease diagnosis, prognosis, or treatment.
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公开(公告)号：US10067139B2
公开(公告)日：2018-09-04
申请号：US14373835
申请日：2013-02-01
发明人： Daniel T. Chiu , Yuhui Jin , Fangmao Ye , Changfeng Wu , Yang-Hsiang Chan
摘要： Polymer nanoparticles and related methods include polymer dots having a coating including a polyelectrolyte polymer. The polymer dots can have a polyelectrolyte coating that can improve colloidal stability of the particles as compared to polymer dots not having the coating. A method of preparing a population of nanoparticles. The methods can include, e.g., providing the population of nanoparticles having a condensed semiconducting polymer; and combining, in a first aqueous solution comprising polyelectrolytes, the population of nanoparticles having the condensed semiconducting polymer to form a population of nanoparticles having a polyelectrolyte coating surrounding the condensed semiconducting polymer of each of the nanoparticles in the population. The methods can include a step of forming the condensed semiconducting polymer using nanoprecipitation or miniemulsion techniques. The polyelectrolyte coating can completely surround the condensed semiconducting polymer.
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