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公开(公告)号：WO2019147310A9
公开(公告)日：2019-08-01
申请号：PCT/US2018/049281
申请日：2018-08-31
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Abstract: Aspects of the present disclosure relate to filtration systems and methods for production of biologically-produced products, which may include pharmaceutical and/or protein products. Certain biomanufacturing systems described herein comprise a bioreactor (e.g., a perfusion bioreactor, a chemostat) and a filter probe comprising a filter bundle comprising a plurality of hollow fibers (e.g., longitudinally aligned hollow fibers). According to some embodiments, a center-to-center distance between any two hollow fibers within the fiber bundle at one or more points along a length of the fiber bundle is relatively large (e.g., greater than or equal to an average outer diameter of the hollow fibers of the fiber bundle, greater than or equal to 1.1 times a minimum diameter of the two hollow fibers). In some embodiments, the hollow fibers within the fiber bundle are arranged in an array (e.g., a hexagonal, linear, annular, or square array).
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2.发明申请
公开(公告)号：WO2019147310A3
公开(公告)日：2019-08-01
申请号：PCT/US2018/049281
申请日：2018-08-31
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
IPC: B01D63/02
Abstract: Aspects of the present disclosure relate to filtration systems and methods for production of biologically-produced products, which may include pharmaceutical and/or protein products. Certain biomanufacturing systems described herein comprise a bioreactor (e.g., a perfusion bioreactor, a chemostat) and a filter probe comprising a filter bundle comprising a plurality of hollow fibers (e.g., longitudinally aligned hollow fibers). According to some embodiments, a center-to-center distance between any two hollow fibers within the fiber bundle at one or more points along a length of the fiber bundle is relatively large (e.g., greater than or equal to an average outer diameter of the hollow fibers of the fiber bundle, greater than or equal to 1.1 times a minimum diameter of the two hollow fibers). In some embodiments, the hollow fibers within the fiber bundle are arranged in an array (e.g., a hexagonal, linear, annular, or square array).
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公开(公告)号：WO2018132635A9
公开(公告)日：2018-07-19
申请号：PCT/US2018/013443
申请日：2018-01-12
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventor： GIERAHN, Todd, Michael , TU, Ang , LOVE, J., Christopher
Abstract: The invention includes methods for enriching for T cell receptor- or B cell receptor-encoding transcripts from a single cell RNA sequencing library, (ii) sequencing T cell receptor- or B cell receptor-encoding transcripts from a single cell RNA sequencing library, and (iii) targeted tagmentation of T cell receptor- or B cell receptor-encoding transcripts.
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公开(公告)号：WO2015023982A1
公开(公告)日：2015-02-19
申请号：PCT/US2014/051343
申请日：2014-08-15
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventor： SHAREI, Armon, R. , ADALSTEINSSON, Viktor, A. , CHO, Nahyun , LANGER, Robert, S. , LOVE, J., Christopher , JENSEN, Klavs, F.
IPC: B07B1/00
CPC classification number: B01L3/502761 , B01L2200/0652 , B01L2300/08 , B01L2400/0487 , B82Y30/00 , C12N5/0634 , C12N5/0693 , C12Q1/04 , G01N1/30 , G01N15/1459 , G01N15/1484 , G01N33/574 , G01N2015/0065 , G01N2015/1006 , G01N2015/1081
Abstract: Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.
Abstract translation: 基于所述细胞的物理性质分离或鉴定细胞可以包括提供细胞悬浮液; 将所述悬浮液通过包括收缩的微流体通道; 使细胞悬液通过狭窄; 并使所述细胞悬浮液与化合物接触。 与相对较小的细胞相比，收缩部的尺寸可以优先变形相对较大的细胞。
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公开(公告)号：WO2022159910A1
公开(公告)日：2022-07-28
申请号：PCT/US2022/013769
申请日：2022-01-25
Applicant: THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , THE GENERAL HOSPITAL CORPORATION
Inventor： LOVE, J., Christopher , MARTIN, Alonso, Maria Carmen , BHATIA, Sangeeta, N. , TABRIZI, Shervin , ADALSTEINSSON, Viktor, A. , XIONG, Kan
IPC: C12N15/10 , C08L101/12 , C12Q1/6825 , G01N33/551
Abstract: This disclosure provides a method for substantially increasing the concentration of cfDNA in a patient. By injecting a patient with lipid and/or polymer nanoparticles, agents that bind cfDNA, or inhibit deoxyribonucleases prior to collection of a sample of cfDNA, e.g., by way of a liquid biopsy, major pathways for the degradation of cfDNA are temporarily blocked, permitting transient accumulation of cfDNA. This strategy has the potential to dramatically enhance the quality of detection achieved by downstream cfDNA analytical applications, such as sequencing applications.
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Patent Agency Ranking
公开(公告)号：WO2018183848A8
公开(公告)日：2018-10-04
申请号：PCT/US2018/025406
申请日：2018-03-30
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventor： LOVE, J., Christopher , MATTHEWS, Catherine, Bartlett , LOVE, Kerry, R. , KUO, Angel
Abstract: Disclosed is media for cultivating cells, e.g., Pichia Pastoris cells, as well as cultures containing cells, methods for making and using the media, and kits comprising the media. The media is particularly useful in the context of therapeutic protein production.
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公开(公告)号：WO2018132635A8
公开(公告)日：2018-07-19
申请号：PCT/US2018/013443
申请日：2018-01-12
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventor： GIERAHN, Todd, Michael , TU, Ang , LOVE, J., Christopher
Abstract: The invention includes methods for enriching for T cell receptor- or B cell receptor-encoding transcripts from a single cell RNA sequencing library, (ii) sequencing T cell receptor- or B cell receptor-encoding transcripts from a single cell RNA sequencing library, and (iii) targeted tagmentation of T cell receptor- or B cell receptor-encoding transcripts.
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公开(公告)号：WO2019113457A1
公开(公告)日：2019-06-13
申请号：PCT/US2018/064494
申请日：2018-12-07
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventor： LOVE, J., Christopher , GIERAHN, Todd, Michael , SHALEK, Alexander, K. , WADSWORTH, Marc, Havens , HUGHES, Travis, K.
IPC: B01L3/00 , C12Q1/6806 , C12N15/10 , C12M1/12 , G03F7/00
CPC classification number: C12Q1/6874 , B01L3/5085 , B01L3/50855 , B01L2200/0647 , B01L2300/0681 , B01L2300/0819 , B01L2300/0829 , B01L2300/0851 , C12N15/1068 , C12N15/1093 , C12N15/1096 , C12Q1/6806 , G03F7/2014 , C12Q2525/15 , C12Q2525/155 , C12Q2525/191 , C12Q2533/101 , C12Q2563/179 , C12Q2525/173
Abstract: This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and ease of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.
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公开(公告)号：WO2018183848A1
公开(公告)日：2018-10-04
申请号：PCT/US2018/025406
申请日：2018-03-30
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventor： LOVE, J., Christopher , MATTHEWS, Catherine, Bartlett , LOVE, Kerry, R. , KUO, Angel
Abstract: Disclosed is media for cultivating cells, e.g., Pichia Pastoris cells, as well as cultures containing cells, methods for making and using the media, and kits comprising the media. The media is particularly useful in the context of therapeutic protein production.
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公开(公告)号：WO2019147310A2
公开(公告)日：2019-08-01
申请号：PCT/US2018/049281
申请日：2018-08-31
Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Abstract: Aspects of the present disclosure relate to filtration systems and methods for production of biologically-produced products, which may include pharmaceutical and/or protein products. Certain biomanufacturing systems described herein comprise a bioreactor (e.g., a perfusion bioreactor, a chemostat) and a filter probe comprising a filter bundle comprising a plurality of hollow fibers (e.g., longitudinally aligned hollow fibers). According to some embodiments, a center-to-center distance between any two hollow fibers within the fiber bundle at one or more points along a length of the fiber bundle is relatively large (e.g., greater than or equal to an average outer diameter of the hollow fibers of the fiber bundle, greater than or equal to 1.1 times a minimum diameter of the two hollow fibers). In some embodiments, the hollow fibers within the fiber bundle are arranged in an array (e.g., a hexagonal, linear, annular, or square array).
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