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公开(公告)号：WO2010072226A1
公开(公告)日：2010-07-01
申请号：PCT/DK2009/050353
申请日：2009-12-23
IPC分类号： C12N9/24
CPC分类号： C12N9/248
摘要： Polypeptides with xylanase activity modified to increase bran solubilisation and/or xylanase activity. The modification comprises modification of one or more amino acids in position 113, 122 or 175 in combination with one or more further amino acid modifications in position 11, 12, 13, 34, 54, 77, 81, 82, 104, 110, 113, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding to the position of Bacillus subtilis xylanase (SEQ ID NO 1).
摘要翻译： 具有木聚糖酶活性的多肽被修饰以增加麸质增溶和/或木聚糖酶活性。 该修饰包括与位置11,12,13,44,57,78,81,82,104,110,113中的一个或多个其它氨基酸修饰相结合的位置113,122或175中的一个或多个氨基酸的修饰 ，118,122,141,154,159,162,164,166,175或179，其中所述位置被确定为对应于枯草芽孢杆菌木聚糖酶（SEQ ID NO 1）的位置的位置。
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公开(公告)号：WO2011045629A1
公开(公告)日：2011-04-21
申请号：PCT/IB2009/054535
申请日：2009-10-15
申请人： DANISCO A/S , BRUNSTEDT, Janne , SØRENSEN, Jens, Frisbæk , SØE, Jørn, Borch , VEDEL, Charlotte, Johansen , WITTSCHIEBEN, Birgitte, Ø. , MIKKELSEN, Jørn, Dalgaard , POULSEN, Charlotte, Horsmans , JENSEN, Lene, B. , MILLER, Lone, B. , LARSEN, Morten, K , LORENTZEN, Rikke, H. , THOUDAHL, Charlotte, R. , CERVIN, Marguerite, A. , BOTT, Richard, R.
发明人： BRUNSTEDT, Janne , SØRENSEN, Jens, Frisbæk , SØE, Jørn, Borch , VEDEL, Charlotte, Johansen , WITTSCHIEBEN, Birgitte, Ø. , MIKKELSEN, Jørn, Dalgaard , POULSEN, Charlotte, Horsmans , JENSEN, Lene, B. , MILLER, Lone, B. , LARSEN, Morten, K , LORENTZEN, Rikke, H. , THOUDAHL, Charlotte, R. , CERVIN, Marguerite, A. , BOTT, Richard, R.
CPC分类号： C12N9/1029 , A21D8/02 , A23V2002/00 , C12N15/00
摘要： In one aspect of the present invention there is provided a method for preparing a variant lipid acyltransferase enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a parent lipid acyltransferase and comprises at least one modification (suitably at least two modifications) at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6. Preferable modifications are one or more of the following L3 IQ, H, N, T, F, Y or C (preferably L31 Q); M27R, G, H, K, Y, D, N, V, C, Q, L, E, S or F (preferably M27V); V85H, R5 D or E; I86R,Y, S, V, I, As T, M, F, C or L (preferably I86S or A); Al 19T or I; Y120K or E; W122S, L or A (preferably W122L); E201R; Q245S; F235A or V; W232G or S; and/or A236G or E.
摘要翻译： 在本发明的一个方面，提供了一种制备变体脂质酰基转移酶的方法，包括在宿主生物体中表达与编码亲本脂质酰基转移酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包含至少一个修饰 （适当地至少两个修饰）在编码的氨基酸序列中对应的位置与位于a）谷氨酸区域的氨基酸（即，优选氨基酸残基31,27,85， 86,119和120）; 和/或b）插入位点1（即氨基酸残基22-36）和/或c）插入位点2（即氨基酸残基74-88），其中峡谷区，插入位点1和/或插入位点2是 定义为当基于一级或三级结构对准的区域对应于如本文所示的酶的SEQ ID No.16或6的峡谷区，插入位点1或插入位点2（或上文所述的相应的氨基酸残基）。 优选的修饰是以下L3 IQ，H，N，T，F，Y或C（优选L31Q）中的一种或多种; M27R，G，H，K，Y，D，N，V，C，Q，L，E，S或F（优选M27V）; V85H，R5 D或E; I86R，Y，S，V，I，As T，M，F，C或L（优选I86S或A）; Al 19T或I; Y120K或E; W122S，L或A（优选W122L）; E201R; Q245S; F235A或V; W232G或S; 和/或A236G或E.
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公开(公告)号：WO2003020923A1
公开(公告)日：2003-03-13
申请号：PCT/IB2002/003797
申请日：2002-08-30
IPC分类号： C12N9/24
CPC分类号： C12N9/2482 , C12Y302/01008
摘要： The present invention relates to a variant xylanase polypeptide, or fragment thereof having xylanase activity, comprising one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor and has an altered thermo-sensitivity as compared with the parent xylanase enzyme.
摘要翻译： 本发明涉及具有木聚糖酶活性的变体木聚糖酶多肽或其片段，其包含一个或多个氨基酸修饰，使得多肽或其片段对木聚糖酶抑制剂具有改变的敏感性，并且与 亲本木聚糖酶。
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公开(公告)号：WO2013037933A2
公开(公告)日：2013-03-21
申请号：PCT/EP2012/068041
申请日：2012-09-14
IPC分类号： C12N9/24
CPC分类号： C12N9/2482 , A23L5/25 , C12C11/003 , C12C12/002 , C12N9/24 , C12N9/2408 , C12N9/242 , C12N9/244 , C12P7/06 , C12Y302/01006 , C12Y302/01008 , C12Y302/01014 , D21H17/005 , Y02E50/17
摘要： The present invention relates to new enzymes with improved properties and to compositions comprising these enzymes suitable for use in the production of a food, feed, or malt beverage product,such as in a brewing process.
摘要翻译： 本发明涉及具有改进性质的新型酶以及包含适合用于生产食品，饲料或麦芽饮料产品的这些酶的组合物，例如在酿造方法中。
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5.发明申请ENZYMATIC GENERATION OF OLIGASACCHARIDES FROM CEREALS OR CEREAL BI-STREAMS 审中-公开来自谷物或谷物双歧杆菌的寡糖的酶促生成
公开(公告)号：WO2010081870A1
公开(公告)日：2010-07-22
申请号：PCT/EP2010/050446
申请日：2010-01-15
申请人： Danisco A/S , SØRENSEN, Jens Frisbæk , MIKKELSEN, René , POULSEN, Charlotte Horsmans , KRAGH, Karsten Matthias
发明人： SØRENSEN, Jens Frisbæk , MIKKELSEN, René , POULSEN, Charlotte Horsmans , KRAGH, Karsten Matthias
CPC分类号： A21D2/188 , A21D8/042 , A21D13/02 , A21D13/062 , A23L7/107 , A23L7/109 , A23L7/115 , A23L7/122 , A23L7/13
摘要： The present invention relates to the solubilisation of cereal bran, for preparing compositions comprising soluble fractions of cereal bran and the use of these compositions comprising solubilised cereal bran for the preparation of food products, such as bread.
摘要翻译： 本发明涉及用于制备包含谷物麸的可溶性级分的组合物的谷蛋白的增溶，以及包含增溶的谷类麸皮的这些组合物用于制备食品如面包的用途。
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公开(公告)号：WO2011114251A1
公开(公告)日：2011-09-22
申请号：PCT/IB2011/050869
申请日：2011-03-01
CPC分类号： A23D7/005 , A21D2/183 , A21D2/186 , A21D8/042 , A23D9/007 , A23L5/25 , A23L29/06 , A23V2002/00 , C12Y203/01 , C12Y204/01019 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , A23V2250/5112
摘要： A foodstuff containing an effective amount of: (a) a lipolytic enzyme capable of acting on a lipid substrate to liberate a free fatty acid molecule; and (b) a cyclodextrin; is disclosed. Methods of producing the foodstuff, as well as uses of a cyclodextrin for decreasing the free fatty acid content of a foodstuff incorporating a lipolytic enzyme, are also disclosed.
摘要翻译： 一种含有有效量的食品：（a）能够作用于脂质底物以释放游离脂肪酸分子的脂肪分解酶; 和（b）环糊精; 被披露。 还公开了生产食品的方法以及环糊精用于降低含有脂肪分解酶的食品的游离脂肪酸含量的用途。
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公开(公告)号：WO2010143149A3
公开(公告)日：2010-12-16
申请号：PCT/IB2010/052581
申请日：2010-06-10
申请人： DANISCO A/S , SØE, Jørn, Borch , POULSEN, Charlotte, Horsmans , ZARGAHI, Masoud, Rajabi , SØRENSEN, Jens, Frisbæk , JØRGENSEN, Tina , BRUNSTEDT, Janne , MIKKELSEN, Rene , MADRID, Susan, Mampusti
发明人： SØE, Jørn, Borch , POULSEN, Charlotte, Horsmans , ZARGAHI, Masoud, Rajabi , SØRENSEN, Jens, Frisbæk , JØRGENSEN, Tina , BRUNSTEDT, Janne , MIKKELSEN, Rene , MADRID, Susan, Mampusti
摘要： The present invention provides methods and uses for treating pyropheophytin-containing compositions, particularly in order to remove pyropheophytin therefrom. The compositions are typically plant, algal, or bacterial derived products such as vegetable oils. The method comprises a step of contacting the compositions with an enzyme having pyropheophytinase activity. Also provided are related apparatus and products for performing such methods and uses.
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公开(公告)号：WO2010072225A1
公开(公告)日：2010-07-01
申请号：PCT/DK2009/050352
申请日：2009-12-23
IPC分类号： C12N9/24
CPC分类号： C12N9/2482 , C12N9/2434 , Y02E50/16
摘要： Polypeptides with xylanase activity modified to increase bran solubilization and/or xylanase activity. The modification comprises modification of one or more amino acids in position 12 or 13 in combination with one or more further amino acid modifications in position 15, 34, 54, 77, 81, 82, 99, 104, 110, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding the position of Bacillus subtilis xylanase (SEQ ID NO 1).
摘要翻译： 具有木聚糖酶活性的多肽被修饰以增加麸增溶和/或木聚糖酶活性。 修饰包括在位置12或13上与位置15,34,54,77,81,82,99,104,110,113,114,118中的一个或多个其它氨基酸修饰的一个或多个氨基酸的修饰 ，122,141,154,159,162,164,166,175或179，其中所述位置被确定为与枯草芽孢杆菌木聚糖酶（SEQ ID NO 1）的位置相对应的位置。
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公开(公告)号：WO2010072224A1
公开(公告)日：2010-07-01
申请号：PCT/DK2009/050351
申请日：2009-12-23
IPC分类号： C12N9/24
CPC分类号： C12N9/2482 , A23L29/06 , A61K38/47 , C12N9/248 , C12Y302/01008
摘要： Polypeptides with xylanase activity modified to increase bran solubilisation and/or xylanase activity. The modification comprises at least an amino acid modification i position 110 and may have further modifications of one or more amino acids in position 11, 12, 13, 34, 54, 77, 81, 99, 104, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166 or 175 wherein the positions are determined as the position corresponding the position of Bacillus subtilis xyianase (SEQ ID NO 1) The polypeptides have at least 88% identity with SEQ ID NO 1 or 75% identity to a sequence selected from SEQ ID NO 2-22.
摘要翻译： 具有木聚糖酶活性的多肽被修饰以增加麸质增溶和/或木聚糖酶活性。 该修饰包含至少一个氨基酸修饰i位置110，并且可以在11位，12位，13个，34个，54个，77个，81个，99个，104个，113个，114个，118个，122个 ，141,154,159,162,164,166或175，其中所述位置被确定为对应于枯草芽孢杆菌木糖酶（SEQ ID NO 1）的位置的位置。多肽与SEQ ID NO 1或75具有至少88％的同一性 与SEQ ID NO 2-22的序列具有同一性。
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公开(公告)号：WO2011124678A1
公开(公告)日：2011-10-13
申请号：PCT/EP2011/055502
申请日：2011-04-08
IPC分类号： A23L1/10
CPC分类号： C12Y302/01008 , A23L7/107 , A23L7/115 , C12Y302/01004 , C12Y302/01015 , C12Y302/01021 , C12Y302/01055 , C12Y302/01089 , C12Y302/01091
摘要： The present invention relates to a method for the modification of cereal bran, a method for the production of a modified cereal bran product as well as the use of such products in the production of food products.
摘要翻译： 本发明涉及一种改性谷糠的方法，一种改性谷物麸产品的生产方法以及这些产品在食品生产中的应用。
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