METHODS FOR FERMENTING CARBOHYDRATE-RICH CROPS
    1.
    发明申请
    METHODS FOR FERMENTING CARBOHYDRATE-RICH CROPS 审中-公开
    用于发酵碳酸氢盐的方法

    公开(公告)号:WO2016140711A1

    公开(公告)日:2016-09-09

    申请号:PCT/US2015/060765

    申请日:2015-11-14

    IPC分类号: C12P7/06 C08B37/00

    摘要: A method for fermenting carbohydrate-rich crops is provided. Sugar beet, sugar cane, sweet sorghum, tropical maize hybrids and fruits are rich in simple sugars; potato, sweet potato, cassava and yam are rich in starch; and Jerusalem artichoke is rich in inulin. This method uses vacuum infusion to infuse yeast into the intercellular space (apoplast) of the parenchyma tissue. The simple sugars diffuse into the apoplast, come into contact with the yeast and produce ethanol. Ethanol can be extracted from the crop by vacuum stripping or crushing or can be left inside the starchy crop to preserve it. In some variants, pectinase enzymes degrade the parenchyma cell walls to speed up diffusion of simple sugars to the yeast, speed up diffusion of amylase to starch granules or speed up diffusion of inulinase to insoluble inulin.

    摘要翻译: 提供了一种发酵富含碳水化合物的作物的方法。 甜菜,甘蔗,甜高粱,热带玉米杂种和水果富含简单的糖类; 土豆,甘薯,木薯,山药均含淀粉; 而菊芋菊粉富含菊粉。 该方法使用真空输注将酵母浸入实质组织的细胞间隙(质外体)中。 简单的糖分散入原生质体,与酵母接触并产生乙醇。 可以通过真空剥离或破碎从作物中提取乙醇,也可以留在淀粉作物中以保存。 在一些变体中,果胶酶降解薄壁细胞壁以加速单糖向酵母的扩散,加速淀粉酶向淀粉颗粒的扩散,或加快菊粉酶向不溶性菊粉的扩散。

    一种两亲性氨基菊糖及其制备
    3.
    发明申请

    公开(公告)号:WO2014071593A1

    公开(公告)日:2014-05-15

    申请号:PCT/CN2012/084311

    申请日:2012-11-08

    IPC分类号: C08B37/00

    CPC分类号: C08B37/0054

    摘要: 本发明公开了一种两亲性氨基菊糖及其制备方法。两亲性氨基菊糖如式(1)所示,其中,R为H、CH 3 、CH 3 CH 2 CH 2 、(CH 3 ) 2 CH或C 6 H 5 ,n的平均取值范围是10-35。两亲性氨基菊糖通过如下方法制备:将菊糖的伯羟基进行卤代反应,反应后与叠氮钠或叠氮锂在40-70°C条件下反应8-24h,经纯化得6叠氮-3,4-二酰基-6脱氧菊糖,6叠氮-3,4-二酰基-6脱氧菊糖经三苯基膦还原即得两亲性氨基菊糖。

    FRUCTOOLIGOSACCHARIDE COMPOSITION, PROCESS FOR ITS PRODUCTION AND USE
    8.
    发明申请
    FRUCTOOLIGOSACCHARIDE COMPOSITION, PROCESS FOR ITS PRODUCTION AND USE 审中-公开
    木糖苷组合物及其生产和使用的方法

    公开(公告)号:WO2009149940A1

    公开(公告)日:2009-12-17

    申请号:PCT/EP2009/004222

    申请日:2009-06-12

    摘要: The invention relates to a process for the production of a fructooligosaccharide (FOS) composition on industrial scale, comprising the steps of: a) providing a raw material containing sucrose; b) bringing the raw material together with an enzyme to form a mixture, whereby the enzyme has an endo-inulinase activity and/or a fructosyltransferase activity and is in free, non-immobilized form; c) exposing the mixture to conditions whereby FOS-forming can take place for an amount of time such that the FOS in the mixture constitutes at least 45 wt. % of the total amount of carbohydrates in the mixture, thereby forming the FOS composition; d) optionally deactivating the enzyme; e) recovering the FOS composition. The invention further relates to Fructooligosaccharide composition (FC) wherein: a portion of at most 2 wt.% of the carbohydrates in the FC consists of oligosaccharides that have a degree of polymerisation (DP) of 7 or more; and at least 56 wt.% of the fructooligosaccharides in the FC have a DP of 3 as measured in HPLC; and the FC is obtained from sucrose in an enzymatic process, whereby no enzyme is used that is obtained from a genetically-modified organism.

    摘要翻译: 本发明涉及一种以工业规模生产低聚果糖(FOS)组合物的方法,包括以下步骤:a)提供含有蔗糖的原料; b)将原料与酶一起形成混合物,由此该酶具有内切菊糖酶活性和/或果糖基转移酶活性,并且是游离的,非固定的形式; c)将混合物暴露于其中FOS形成可以发生一段时间的条件,使得混合物中的FOS构成至少45重量% 混合物中碳水化合物总量的百分比,从而形成FOS组合物; d)任选地使酶失活; e)回收FOS组合物。 本发明还涉及低聚果糖组合物(FC),其中:FC中至多2wt%的碳水化合物的一部分由聚合度(DP)为7或更高的寡糖组成; 并且FC中至少56wt。%的低聚果糖的DP为3; 并且在酶处理中从蔗糖获得FC,由此不能使用从遗传修饰的生物获得的酶。

    VERFAHREN ZUR EXTRAKTION VON MEMBRANPROTEINEN
    9.
    发明申请
    VERFAHREN ZUR EXTRAKTION VON MEMBRANPROTEINEN 审中-公开
    膜蛋白的方法提取

    公开(公告)号:WO2009077046A1

    公开(公告)日:2009-06-25

    申请号:PCT/EP2008/009704

    申请日:2008-11-17

    IPC分类号: G01N33/68

    摘要: Die vorliegende Erfindung betrifft die Verwendung von linearen amphipathischen Kohlenhydrat-Polymeren zur Extraktion von Membranproteinen aus biologischen Proben, Verfahren zur Extraktion von Membranproteinen, ein Kit zur Extraktion dieser Proteine sowie dessen Verwendung.

    摘要翻译: 本发明涉及使用线性两亲性的碳水化合物聚合物的膜蛋白的从生物样品,膜蛋白,这些蛋白质和其使用的提取试剂盒的提取方法的提取。

    NON-BITTER CHICORY EXTRACT
    10.
    发明申请
    NON-BITTER CHICORY EXTRACT 审中-公开
    非TER子提取物

    公开(公告)号:WO0213625A3

    公开(公告)日:2002-07-18

    申请号:PCT/EP0109261

    申请日:2001-08-09

    发明人: CRELIER SIMON

    摘要: The present invention relates to processes for the production of a non-bitter chicory extract comprising subjecting an aqueous chicory extract to UV treatment and alkaline treatment or subjecting an aqueous chicory extract with a pH of below 7 to a UV treatment or subjecting an aqueous chicory extract with a pH of below 7 to UV treatment or subjecting an aqueous chicory extract to an alkaline treatment at a pH greater than 10, at a temperature of 0 to 75 DEG C and for a time of at least 15 minutes. The present invention also relates to a further process for the preparation of a non-bitter chicory extract comprising subjecting an aqueous chicory extract to ethanol precipitation, separating the precipitate by filtration and drying the precipitate.

    摘要翻译: 本发明涉及生产非苦瓜菊苣提取物的方法,包括使水性菊苣提取物经受UV处理和碱处理,或使pH值低于7的水性菊苣萃取液进行紫外线处理或使水性菊苣萃取液 pH值低于7以进行紫外线处理,或者在0至75℃的温度和至少15分钟的时间内,使pH值大于10的水性菊苣萃取液进行碱处理。 本发明还涉及一种制备非苦涩菊苣提取物的另一种方法,其包括使水性菊苣萃取物经乙醇沉淀,通过过滤分离沉淀物并干燥沉淀物。