公开(公告)号：WO1991016449A1

公开(公告)日：1991-10-31

申请号：PCT/US1991002430

申请日：1991-04-11

Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

Inventor： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA ,  ROTH, Steve

IPC: C12P21/100

CPC classification number: C12N11/00 ,  C12N9/1051 ,  C12P19/18 ,  C12P19/26 ,  Y10S435/815 ,  Y10S435/819

Abstract: A method for preparing saccharide compositions is disclosed. The method is reiterative and comprises the following three steps: (i) a glycosyltransferase capable of transfering a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltranferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.

Abstract translation: 公开了制备糖组合物的方法。 该方法是重复的并且包括以下三个步骤：（i）能够将预选的糖单元转移到受体部分的糖基转移酶通过在有效结合受体部分的条件下使受体部分与疑似含有糖基转移酶的混合物接触来分离 和糖基转移酶，从而分离糖基转移酶。 受体部分是蛋白质，糖蛋白，脂质，糖脂或碳水化合物。 （ii）然后将分离的糖基转移酶用于催化受体部分和预选糖单元之间的键。 （iii）使用在该方法的第一次迭代中获得的中间产物作为第二次迭代的受体部分，重复步骤（i）和（ii）。

公开(公告)号：WO1991016340A1

公开(公告)日：1991-10-31

申请号：PCT/US1991002650

申请日：1991-04-18

Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM ,  BROWN, Michael, S. ,  GOLDSTEIN, Joseph, L. ,  REISS, Yuval

Inventor： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

IPC: C07K07/06

CPC classification number: C07K7/06 ,  A61K38/00 ,  C07K1/1077 ,  C07K5/1013 ,  C07K5/1016 ,  C07K5/1019 ,  C07K5/1024 ,  C07K7/02 ,  C07K14/82 ,  C12N9/1085 ,  Y10S435/815

Abstract: Disclosed are methods and compositions for the identification, characterization and inhibition of farnesyl protein transferases, enzymes involved in the farnesylation of various cellular proteins, including cancer related ras proteins such as p21ras. One farnesyl protein transferase which is disclosed herein exhibits a molecular weight of between about 70,000 and about 100,000 upon gel exclusion chromatography. The enzyme appears to comprise one or two subunits of approximately 50 kDa each. Methods are disclosed for assay and purification of the enzyme, as well as procedures for using the purified enzyme in screening protocols for the identification of possible anticancer agents which inhibit the enzyme and thereby prevent expression of proteins such as p21ras. Also disclosed is a family of compounds which act either as false substrates for the enzyme or as pure inhibitors and can therefore be employed for inhibition of the enzyme. The most potent inhibitors are ones in which phenylalanine occurs at the third position of a tetrapeptide whose amino terminus is cysteine.

公开(公告)号：WO1991002790A1

公开(公告)日：1991-03-07

申请号：PCT/JP1990001036

申请日：1990-08-14

Applicant: SHISEIDO COMPANY, LTD. ,  IIDA, Toshii ,  KAMINUMA, Toshihiko ,  FUSE, Yuka ,  TAJIMA, Masahiro ,  YANAGI, Mitsuo ,  OKAMOTO, Hiroshi ,  KISHIMOTO, Jiro ,  IFUKU, Ohji ,  KATO, Ichiro

Inventor： SHISEIDO COMPANY, LTD.

IPC: C12N09/02

CPC classification number: C12N9/0071 ,  C07K1/107 ,  C12Y114/17003 ,  Y10S435/814 ,  Y10S435/815

Abstract: An enzyme which acts on a peptide C-terminal/glycine adduct of formula (I), (wherein A represents a residue formed by removing an alpha -amino or imino group and an alpha -carboxyl group from a natural alpha -amino acid, and X represents a hydrogen atom or a residue of an amino acid derivative which combines with the N atom via a carbonyl group) to produce a peptide C-terminal/ alpha -hydroxyglycine adduct of formula (II), (wherein A and X are each as defined above), and another enzyme which acts on the compound of formula (II) to produce a C-terminal amidated peptide of formula (III), wherein A and X are each as defined above.

Abstract translation: 作用于式（I）的肽C-末端/甘氨酸加合物的酶（其中A表示通过从天然α-氨基酸除去α-氨基或亚氨基和α-羧基形成的残基，以及 X表示氢原子或通过羰基与N原子结合的氨基酸衍生物的残基），以制备式（II）的肽C-末端/α-羟基甘氨酸加合物（其中A和X各自为 以及另一种作用于式（II）化合物以产生式（III）的C-末端酰胺化肽的酶，其中A和X各自如上所定义。

公开(公告)号：WO1989008702A1

公开(公告)日：1989-09-21

申请号：PCT/US1989000936

申请日：1989-03-08

Applicant: CODON

Inventor： CODON ,  RICE, Craig ,  MORSER, Michael, John ,  GLASER, Charles

IPC: C12N09/48

CPC classification number: C12N9/6459 ,  C12Y304/21069 ,  Y10S435/815 ,  Y10S514/824

Abstract: This invention relates to the efficient recovery of tissue plasminogen activator (t-PA) from liquid media and more specifically, to improved methods for recovering intact single-chain t-PA substantially free of degraded t-PA and other non-homologous proteins using chaotropic agents to stabilize high concentrations of t-PA during purification.

Abstract translation: 本发明涉及从液体培养基有效回收组织纤溶酶原激活剂（t-PA），更具体地说，涉及用于回收基本上不含降解的t-PA的完整单链t-PA和使用离液序列的其它非同源蛋白质的改进方法 在纯化期间稳定高浓度的t-PA的试剂。

公开(公告)号：WO1984002720A1

公开(公告)日：1984-07-19

申请号：PCT/EP1984000004

申请日：1984-01-07

Applicant: BOEHRINGER MANNHEIM GMBH ,  ROSALKI, Sidney, Bertram

Inventor： BOEHRINGER MANNHEIM GMBH

IPC: C12Q01/42

CPC classification number: C12Q1/007 ,  G01N27/44726 ,  Y10S435/80 ,  Y10S435/814 ,  Y10S435/815 ,  Y10S436/827

Abstract: Method and reagent for the differential analysis of the bone and lever isoenzyme of the alkaline phosphatase in a sample, said phosphatase being mixed and incubated with a lectine capable of binding the rests of N-acetylglucosamin. The free isoenzyme portions are separated from the portions bound to the lectine and the activity of the alkaline phosphatase is determined in one of the separated media or in both media.

Abstract translation: 用于差异分析样品中碱性磷酸酶的骨和杆同工酶的方法和试剂，将所述磷酸酶混合并与能够结合其余N-乙酰葡糖胺的凝固素一起温育。 游离同工酶部分与结合到凝胶素的部分分离，碱性磷酸酶的活性在分离的培养基之一或两种培养基中测定。

公开(公告)号：WO00065352A1

公开(公告)日：2000-11-02

申请号：PCT/US2000/011708

申请日：2000-04-28

IPC: A61L27/34 ,  A61L31/10 ,  G01N33/543 ,  G01N33/551

CPC classification number: B82Y30/00 ,  A61B5/14546 ,  A61B5/1459 ,  A61L27/34 ,  A61L31/10 ,  G01N33/54373 ,  G01N33/54393 ,  G01N33/551 ,  Y10S435/815 ,  Y10S435/961 ,  Y10S435/969 ,  Y10S436/819 ,  Y10S436/823

Abstract: Multifunctional, polyionic copolymers are synthesized on/or applied to substrate surfaces for analytical and sensing purposes, the coatings being particularly useful for suppression of non-specific interaction, adsorption or attachment. Groups can be coupled to, integrated into or absorbed to the multifunctional polymer that are able to recognize, interact with and bind specifically to target analyte molecules. These materials can also be used to modulate biological interactions upon substrate surfaces for use as selective implant surfaces that resist cell attachment and may optionally promote the attachment of specific cell types or induce a particular cellular behaviour. The multifunctional polymer coatings typically include brush copolymers based on a polycationic or polyanionic backbone with side chains that control interaction with the environment, such as poly(ethylene glycol) or poly(ethylene oxide)-based side chains that decrease cellular adhesion, and analyte-specific side chains.

Abstract translation: 多功能聚异氰酸酯共聚物在/或应用于底物表面上用于分析和检测目的，该涂料特别适用于抑制非特异性相互作用，吸附或附着。 组可以结合到，整合或吸收到能够识别，与靶分析物分子特异性相互作用并与其结合的多功能聚合物。 这些材料也可用于调节基底表面上的生物相互作用，用作抵抗细胞附着的选择性植入物表面，并且可任选地促进特定细胞类型的附着或诱导特定的细胞行为。 多官能聚合物涂层通常包括基于聚阳离子或聚阴离子主链的刷共聚物，侧链具有控制与环境相互作用的侧链，例如降低细胞粘附性的聚（乙二醇）或聚（环氧乙烷） - 侧链， 具体侧链。

公开(公告)号：WO99040122A1

公开(公告)日：1999-08-12

申请号：PCT/EP1999/000635

申请日：1999-02-01

IPC: C07K17/02 ,  C07K17/08 ,  C08F2/18 ,  C08F2/32 ,  C08F220/00 ,  C08F220/32 ,  C08F220/56 ,  C12N11/08 ,  C12P41/00

CPC classification number: C07K17/08 ,  C08F2/32 ,  C12N11/08 ,  Y10S435/803 ,  Y10S435/815 ,  Y10S530/815

Abstract: The invention relates to a method for producing a bead-shaped, cross-linked hydrophilic mixed polymer which actively bonds with ligands with nucleophilic groups, by inversely bead-polymerising a monomer phase. The invention also relates to polymer support materials with a high capacity for bonding with penicillin acylase and with a lower swelling number, and to their use.

Abstract translation: 本发明涉及一种用于球形交联的亲水的制造方法，与用亲核基团的配体，通过单体的逆珠聚合结合活性共聚物。 本发明涉及一种载体聚合物材料具有用于青霉素酰化酶和低溶胀指数以及它们的用途具有高结合容量。

公开(公告)号：WO98059361A1

公开(公告)日：1998-12-30

申请号：PCT/US1998/012907

申请日：1998-06-19

IPC: G01N33/52 ,  A61K49/00 ,  B01D15/38 ,  B01J20/281 ,  B01J20/286 ,  B01J20/32 ,  C12M1/34 ,  C12M1/42 ,  C12N15/09 ,  C12Q1/00 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/10 ,  G01N27/62 ,  G01N27/64 ,  G01N30/00 ,  G01N30/02 ,  G01N30/46 ,  G01N30/90 ,  G01N30/95 ,  G01N33/15 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/531 ,  G01N33/538 ,  G01N33/543 ,  G01N33/566 ,  G01N33/68 ,  G01N37/00 ,  G06F19/00 ,  H01J49/04 ,  H01J49/26

CPC classification number: C40B30/04 ,  B01D15/1878 ,  B01D15/305 ,  B01D15/3809 ,  B01J20/281 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/3253 ,  B01J20/3265 ,  B01J20/3274 ,  B01J20/3289 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2220/54 ,  C12Q1/00 ,  C40B40/10 ,  G01N30/00 ,  G01N30/02 ,  G01N30/461 ,  G01N30/463 ,  G01N30/466 ,  G01N30/48 ,  G01N30/482 ,  G01N30/90 ,  G01N30/95 ,  G01N33/531 ,  G01N33/538 ,  G01N33/543 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/54393 ,  G01N33/566 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/6851 ,  G01N2030/008 ,  G01N2030/009 ,  Y02A90/26 ,  Y10S422/906 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S435/814 ,  Y10S435/815 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/824 ,  Y10S436/825 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  B01D15/363 ,  B01D15/322 ,  B01D15/327 ,  B01D15/3804 ,  B01D15/362 ,  B01D15/38 ,  B01D15/26

Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.

Abstract translation: 本发明提供了用于分析样品中分析物的滞留层析方法。 所述方法包括在多种不同的选择性条件下将分析物吸附到底物上，并通过解吸光谱法检测保留在底物上的分析物。 这些方法在生物学和医学中是有用的，包括临床诊断和药物发现。

公开(公告)号：WO1998003638A1

公开(公告)日：1998-01-29

申请号：PCT/AU1997000452

申请日：1997-07-18

Applicant: THE AUSTRALIAN NATIONAL UNIVERSITY ,  FREEMAN, Craig, Geoffrey ,  PARISH, Christopher, Richard

Inventor： THE AUSTRALIAN NATIONAL UNIVERSITY

IPC: C12N09/24

CPC classification number: C12Y302/01031 ,  C12N9/2402 ,  C12Q1/34 ,  C12Y302/01166 ,  Y10S435/814 ,  Y10S435/815

Abstract: A method for detecting mammalian heparanase activity in a sample such as mammalian tissue, cells or bodily fluids; and a method for the purification of mammalian heparanase from a heparanase-containing material, such as human platelets.

Abstract translation: 用于检测样品如哺乳动物组织，细胞或体液中哺乳动物肝素酶活性的方法; 以及从含有肝素酶的材料如人血小板纯化哺乳动物肝素酶的方法。

公开(公告)号：WO1997032015A1

公开(公告)日：1997-09-04

申请号：PCT/EP1997000755

申请日：1997-02-18

Applicant: KNOLL AKTIENGESELLSCHAFT ,  SCHWARZ, Margarete ,  ZAHN, Wolfgang

Inventor： KNOLL AKTIENGESELLSCHAFT

IPC: C12N09/64

CPC classification number: C12N9/6418 ,  Y10S435/814 ,  Y10S435/815

Abstract: Described is a method of purifying thrombin-like protease enzymes from snake venom, the method consisting of freeing the enzymes of impurities in three chromatographic stages.

Abstract translation: 提供了一种用于净化从描述蛇毒，这是它释放以杂质三个色谱步骤的蛋白酶凝血酶样蛋白酶的方法。