公开(公告)号：EP2821789A1

公开(公告)日：2015-01-07

申请号：EP13003337.6

申请日：2013-07-02

申请人： ETH Zurich

发明人： Millan, Christopher ,  Miranda-Nieves, David ,  Zenobi-Wong, Marcy ,  Yang, Yuan ,  Groth, Thomas

IPC分类号： G01N33/50

CPC分类号： G01N33/5082 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0662 ,  C12N5/0671 ,  C12N2513/00 ,  C12N2533/72 ,  C12N2533/74 ,  C12N2537/10 ,  G01N2500/10

摘要： A method of making a three-dimensional microtissue comprising the reaction of at least two cytocompatible polymer solutions, each polymer comprising complementary reactive groups capable of spontaneous reaction, at least one of which solutions additionally contains cells.
The method allows the rapid, efficient production of microtissues useful for purposes such as pharmaceutical screening and tissue reconstruction.

摘要翻译： 一种制备三维微量组织的方法，其包括至少两种细胞相容性聚合物溶液的反应，每种聚合物包含能够自发反应的互补反应性基团，其中至少一种溶液另外含有细胞。 该方法允许快速，有效地生产用于诸如药物筛选和组织重建等目的的微量组分。

公开(公告)号：EP2653534A4

公开(公告)日：2014-05-14

申请号：EP11848080

申请日：2011-12-07

申请人： SHISEIDO CO LTD

发明人： FUJIWARA SHIGEYOSHI ,  TAKEI KEIGO ,  SUDA YUKIMITSU

IPC分类号： C12N5/071 ,  C12M1/22 ,  C12Q1/02

CPC分类号： G01N33/502 ,  C12M23/20 ,  C12M35/08 ,  C12N5/0068 ,  C12N5/0667 ,  C12N5/0671 ,  C12N5/0693 ,  C12N2533/30 ,  G01N33/5032 ,  G01N33/5082

公开(公告)号：EP2633033A2

公开(公告)日：2013-09-04

申请号：EP11785714.4

申请日：2011-10-26

申请人： UPM-Kymmene Corporation

发明人： YLIPERTTULA, Marjo ,  LAURÉN, Patrick ,  BHATTACHARYA, Madhushree ,  LOU, Yanru ,  LAUKKANEN, Antti

IPC分类号： C12N5/00 ,  A61L27/36 ,  A61L27/38

CPC分类号： C12N5/0671 ,  A61K9/06 ,  A61K47/26 ,  A61K47/38 ,  A61L27/20 ,  A61L27/3637 ,  A61L27/38 ,  A61L27/52 ,  C08J3/075 ,  C08J2301/02 ,  C08J2389/00 ,  C08L1/02 ,  C08L97/02 ,  C12N5/0068 ,  C12N5/0606 ,  C12N5/0621 ,  C12N5/067 ,  C12N5/0672 ,  C12N11/04 ,  C12N11/12 ,  C12N2533/78

摘要： The present invention relates material based on microbial cellulose that is useful in culturing and transferring cells as well as delivering drugs. The material comprises cellulose nanofibers and/or derivatives thereof, based on microbial cellulose, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

公开(公告)号：EP2632493A2

公开(公告)日：2013-09-04

申请号：EP11785715.1

申请日：2011-10-26

申请人： UPM-Kymmene Corporation

发明人： YLIPERTTULA, Marjo ,  LAURÉN, Patrick ,  BHATTACHARYA, Madhushree ,  LOU, Yanru ,  LAUKKANEN, Antti

IPC分类号： A61K47/38 ,  A61L27/52

CPC分类号： C12N5/0671 ,  A61K9/06 ,  A61K47/26 ,  A61K47/38 ,  A61L27/20 ,  A61L27/3637 ,  A61L27/38 ,  A61L27/52 ,  C08J3/075 ,  C08J2301/02 ,  C08J2389/00 ,  C08L1/02 ,  C08L97/02 ,  C12N5/0068 ,  C12N5/0606 ,  C12N5/0621 ,  C12N5/067 ,  C12N5/0672 ,  C12N11/04 ,  C12N11/12 ,  C12N2533/78

摘要： The present invention relates material that is useful in drug delivery compositions. The material comprises cellulose nanofibers and/or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

公开(公告)号：EP2495310A3

公开(公告)日：2013-03-27

申请号：EP12158304.1

申请日：2006-05-24

申请人： The Regents Of the University of California

发明人： Bhatia, Sangeeta N. ,  Khetani, Salman R.

IPC分类号： C12N5/071

CPC分类号： G01N33/5023 ,  C12N5/0656 ,  C12N5/067 ,  C12N5/0671 ,  C12N5/0697 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2533/54 ,  C12N2535/10 ,  G01N33/5014 ,  G01N33/5067

摘要： The disclosure provides an in vitro culture systems. The invention provides methods and systems useful for developing and in vitro engineered tissue, method of using the tissue and compositions comprising the tissue.

公开(公告)号：EP2444477A4

公开(公告)日：2013-01-09

申请号：EP10789497

申请日：2010-06-15

申请人： SHISEIDO CO LTD

发明人： FUJIWARA SHIGEYOSHI ,  TAKEI KEIGO ,  HIRAYAMA AYA ,  SUDA YUKIMITSU

IPC分类号： C12M1/00 ,  C08J7/04 ,  C08J7/12 ,  C09D133/14 ,  C12M3/00 ,  C12N5/07

CPC分类号： C12M23/20 ,  C12N5/062 ,  C12N5/0667 ,  C12N5/0671 ,  C12N5/0693 ,  C12N2533/30 ,  G01N33/5082

摘要： A container for forming a cell aggregate is provided in which a group represented by a general formula of: (in the formula, each of R 1 , R 2 , and R 3 is independently a an alkyl group with a carbon number of 1 or more and 6 or less and m is an integer of 2 or more and 6 or less.) and at least one of an amino group, a carboxyl group, and a hydroxyl group are present near a surface thereof.

公开(公告)号：EP2471908A1

公开(公告)日：2012-07-04

申请号：EP10811643.5

申请日：2010-07-28

申请人： The University of Tokyo ,  Mitsubishi Chemical Medience Corporation

发明人： MATSUI, Hitoshi ,  SAKAI, Yasuyuki ,  FUJII, Teruo ,  TAKEUCHI, Shoji ,  TSUDA, Yukiko

IPC分类号： C12N5/077 ,  C12M1/34 ,  C12Q1/02

CPC分类号： C12M23/20 ,  C12M23/24 ,  C12M25/14 ,  C12M41/46 ,  C12N5/0671 ,  C12N2503/00 ,  C12N2533/30 ,  C12N2533/54 ,  G01N33/5044

摘要： A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.

摘要翻译： 培养肝细胞的方法，其中将包埋在细胞外基质中的肝细胞置于透气膜上，并且在从透气膜侧供给氧的同时培养肝细胞。 由此，可以诱导肝细胞中的极性，并且可以在短时间内形成胆小管。 此外，所形成的极性可以保持更长的时间。

公开(公告)号：EP2443230A2

公开(公告)日：2012-04-25

申请号：EP10730123.6

申请日：2010-06-18

申请人： Cellartis AB

发明人： STACHELSCHEID, Harald ,  ZEILINGER, Katrin ,  JENSEN, Janne ,  URBANIAK, Thomas

IPC分类号： C12N5/071

CPC分类号： C12N5/067 ,  C12M25/10 ,  C12N5/0671 ,  C12N2500/25 ,  C12N2500/34 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/23 ,  C12N2501/39 ,  C12N2501/70 ,  C12N2506/02

摘要： The present invention relates to the use of 3D culturing systems for the derivation of hepatocyte-like cells from human pluripotent stem cells (hPS). In particular, the invention concerns the directed differentiation and maturation of human pluripotent stem cells into hepatocyte like cells in 3D hollow fiber capillary bioreactors.

摘要翻译： 本发明涉及3D培养系统用于从人多能干细胞（hps）衍生的肝细胞样细胞的用途。 特别地，本发明涉及人类多能干细胞在3D中空纤维毛细血管生物反应器中进入肝细胞样细胞的定向分化和成熟。

公开(公告)号：EP2421954A1

公开(公告)日：2012-02-29

申请号：EP10714113.7

申请日：2010-04-21

申请人： Academisch Ziekenhuis bij de Universiteit van Amsterdam

发明人： CHAMULEAU, Robert, Antoine, François, Marie ,  HOEKSTRA, Ruurdtje ,  NIBOURG, Gerardus, Adrianus, Antonius

IPC分类号： C12N5/071 ,  C12M3/00

CPC分类号： A61M1/3621 ,  C12M21/08 ,  C12M25/14 ,  C12M29/00 ,  C12N5/0671 ,  C12N2500/32 ,  C12N2500/46

摘要： The present invention concerns human hepatocyte cell line cultures and their use in bioartificial liver (BAL) systems. These systems are used to treat subjects suffering from liver failure to temporarily compensate for loss of hepatocellular function and generally comprise a bioreactor loaded with functional liver cells. Until now, it has been problematic to acquire cells with a broad spectrum metabolic functionality, resembling that of freshly isolated human hepatocytes, to the extent that they are in fact suitable for successful clinical BAL application The present inventors have managed to develop human hepatocyte cell line cultures that display broad-spectrum metabolic functionality such as to render them particularly suitable for effective clinical BAL application.

摘要翻译： 本发明涉及人肝细胞细胞系培养物及其在生物人造肝（BAL）系统中的应用。 这些系统用于治疗患有肝衰竭的受试者以暂时补偿肝细胞功能的损失，并且通常包含装载功能性肝细胞的生物反应器。 到目前为止，获得具有广谱代谢功能的细胞是类似于新鲜分离的人肝细胞的细胞的一个问题，其程度实际上适合于成功的临床BAL应用本发明人已经设法开发人肝细胞细胞系 显示广谱代谢功能的培养物，使其特别适用于有效的临床BAL应用。

公开(公告)号：EP2245141A4

公开(公告)日：2011-07-27

申请号：EP09702371

申请日：2009-01-21

申请人： UNIV MINNESOTA ,  UNIV LEUVEN KATH

发明人： HU WEI-SHOU ,  SUBRAMANIAN KARTIK ,  VERFAILLIE CATHERINE M

IPC分类号： C12N5/071 ,  C12N5/074

CPC分类号： C12N5/067 ,  A61K35/12 ,  C12N5/0607 ,  C12N5/0671 ,  C12N2500/02 ,  C12N2501/11 ,  C12N2501/135 ,  C12N2501/235 ,  C12N2503/02 ,  C12N2506/03

摘要： The invention is directed to compositions of cell aggregates and methods for making and using the cell aggregates where the aggregates comprise cells that are not embryonic stern cells but can differentiate into cell types of at least two of ectodermal, undo dermal, and mesodermal embryonic germ layers, e.g., stem cells.

摘要翻译： 本发明涉及细胞聚集体的组合物和用于制备和使用细胞聚集体的方法，其中聚集体包含不是胚胎细胞但可分化为至少两种外胚层，去皮肤和中胚层胚胎胚层的细胞类型 ，例如干细胞。