公开(公告)号：US20100317540A1

公开(公告)日：2010-12-16

申请号：US12772829

申请日：2010-05-03

申请人： JOHN MCCAFFERTY ,  ANTHONY RICHARD POPE ,  KEVIN STUART JOHNSON ,  HENDRICUS RENERUS JACOBUS MATTHEUS HOOGENBOOM ,  ANDREW DAVID GRIFFITHS ,  RONALD HENRY JACKSON ,  KASPER PHILIPP HOLLIGER ,  JAMES DAVID MARKS ,  TIMOTHY PIERS CLACKSON ,  DAVID JOHN CHISWELL ,  GREGORY PAUL WINTER ,  TIMOTHY PETER BONNERT

发明人： JOHN MCCAFFERTY ,  ANTHONY RICHARD POPE ,  KEVIN STUART JOHNSON ,  HENDRICUS RENERUS JACOBUS MATTHEUS HOOGENBOOM ,  ANDREW DAVID GRIFFITHS ,  RONALD HENRY JACKSON ,  KASPER PHILIPP HOLLIGER ,  JAMES DAVID MARKS ,  TIMOTHY PIERS CLACKSON ,  DAVID JOHN CHISWELL ,  GREGORY PAUL WINTER ,  TIMOTHY PETER BONNERT

IPC分类号： C40B30/04 ,  C12N15/63 ,  C12N7/01 ,  G01N33/50

CPC分类号： C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

公开(公告)号：US07723270B1

公开(公告)日：2010-05-25

申请号：US09417478

申请日：1999-10-13

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kasper Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kasper Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： C40B40/02 ,  C12P21/08 ,  A23J1/00 ,  C07H21/02 ,  C07H21/00 ,  C12Q1/68

CPC分类号： C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US20090155810A1

公开(公告)日：2009-06-18

申请号：US11555464

申请日：2006-11-01

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Hendricus Renerus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kasper Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Hendricus Renerus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kasper Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： G01N33/53 ,  C12N15/63

CPC分类号： C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US06172197B2

公开(公告)日：2001-01-09

申请号：US08484893

申请日：1995-06-07

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： C07K1600

CPC分类号： C07K16/468 ,  C07K16/18 ,  C07K16/462 ,  C07K2317/24 ,  C07K2319/02 ,  Y10S530/867

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 可以通过与互补sbp成员的亲和力来选择显示的sbps，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合配偶体多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而在sbp成员中获得比通过常规方法容易获得的更大的遗传多样性。

公开(公告)号：US5969108A

公开(公告)日：1999-10-19

申请号：US971857

申请日：1993-01-08

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： C12N1/21 ,  C07H21/00 ,  C07K16/00 ,  C07K16/18 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/46 ,  C07K19/00 ,  C12N7/00 ,  C12N7/01 ,  C12N15/00 ,  C12N15/09 ,  C12N15/10 ,  C12N15/13 ,  C12N15/62 ,  C12N15/73 ,  C12P21/02 ,  C12R1/19 ,  C40B40/02 ,  G01N33/53 ,  G01N33/531 ,  G01N33/68 ,  C07H21/04

CPC分类号： C07K16/005 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  B01J2219/00718 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  Y10S530/867

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： PCT No.PCT / GB91 / 01134 Sec。 371日期：1993年1月8日 102（e）日期1993年1月8日PCT 1991年7月10日PCT公布。 出版物WO92 / 01047 日期1992年1月23日通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定特异性结合对（sbp）的成员，其中sbp成员以分泌重组体的表面的功能形式显示 遗传显示包（rgdp），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US07732377B2

公开(公告)日：2010-06-08

申请号：US10803622

申请日：2004-03-18

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： C40B30/04 ,  C12Q1/70 ,  G01N33/53

CPC分类号： C40B40/02 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  G01N33/53 ,  G01N33/6857

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a filamentous bacteriophage particle containing DNA encoding the sbp member, wherein the sbp member has a binding domain that consists of a dAb fragment. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected filamentous bacteriophage particles for expression of the selected sbp members.

摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这样的sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以功能形式显示在含有编码DNA的DNA的丝状噬菌体颗粒的表面 sbp成员，其中sbp成员具有由dAb片段组成的结合结构域。 可以通过与互补sbp成员的亲和力来选择显示的sbps，并且从所选择的丝状噬菌体颗粒中回收DNA以表达所选择的sbp成员。

公开(公告)号：US07122646B2

公开(公告)日：2006-10-17

申请号：US10247839

申请日：2002-09-20

申请人： Kaspar-Philipp Holliger ,  Andrew David Griffiths ,  Hendricus Renerus Jacobus Matheus Hoogenboom ,  Magnus Malmqvist ,  James David Marks ,  Brian Timothy McGuinness ,  Anthony Richard Pope ,  Terence Derek Prospero ,  Gregory Paul Winter

发明人： Kaspar-Philipp Holliger ,  Andrew David Griffiths ,  Hendricus Renerus Jacobus Matheus Hoogenboom ,  Magnus Malmqvist ,  James David Marks ,  Brian Timothy McGuinness ,  Anthony Richard Pope ,  Terence Derek Prospero ,  Gregory Paul Winter

IPC分类号： C07H21/02

CPC分类号： C12N15/113 ,  A61K38/00 ,  C07K16/00 ,  C07K16/1063 ,  C07K16/241 ,  C07K16/26 ,  C07K16/2809 ,  C07K16/283 ,  C07K16/3007 ,  C07K16/3061 ,  C07K16/40 ,  C07K16/4283 ,  C07K16/44 ,  C07K16/468 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/624 ,  C07K2317/626 ,  C07K2317/73 ,  C07K2319/00

摘要： Polypeptides comprising a first domain, which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain, which comprises a binding region of an immunoglobulin light chain variable region, the domains being linked but incapable of associating with each other to form an antigen binding site, associate to form antigen binding multimers, such as dimers, which may be multivalent or have multispecificity. The domains may be linked by a short peptide linker or may be joined directly together. Bispecific dimers may have longer linkers. Methods of preparation of the polypeptides and multimers and diverse repertoires thereof, and their display on the surface of bacteriophage for easy selection of binders of interest, are disclosed, along with many utilities.

摘要翻译： 包含第一结构域的多肽，其包含免疫球蛋白重链可变区的结合区和第二结构域，其包含免疫球蛋白轻链可变区的结合区，所述结构域被连接但不能彼此缔合形成 抗原结合位点，缔合以形成抗原结合多聚体，例如二聚体，其可以是多价的或具有多特异性。 结构域可以通过短肽接头连接或者可以直接连接在一起。 双特异性二聚体可以具有更长的接头。 公开了多肽和多聚体及其多种组成成分的制备方法，以及它们在噬菌体表面上的显示以便于选择感兴趣的粘合剂，以及许多实用性。

公开(公告)号：US06806079B1

公开(公告)日：2004-10-19

申请号：US09726219

申请日：2000-11-28

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Henricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kaspar Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： C12N1500

CPC分类号： C40B40/02 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  G01N33/53 ,  G01N33/6857

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US5977319A

公开(公告)日：1999-11-02

申请号：US958201

申请日：1997-10-21

申请人： Anthony Richard Pope ,  Kevin Pritchard ,  Andrew James Williams ,  Kevin Stuart Johnson

发明人： Anthony Richard Pope ,  Kevin Pritchard ,  Andrew James Williams ,  Kevin Stuart Johnson

IPC分类号： C07K16/26 ,  C07K16/00 ,  A61K39/395

CPC分类号： C07K16/26 ,  C07K2317/34 ,  C07K2317/622

摘要： The invention relates to specific binding members for estradiol and materials and methods relating thereto, in particular antibodies or binding domains thereof which have high affinities (low dissociation constants) for estradiol and low cross-reactivity for other steroids. The invention further provides means to make such binding members, assay methods for the detection and/or quantitation of estradiol, and nucleic acid encoding said binding members, which nucleic acid may be used for their production. Preferred binding members include those with CDR regions of the D12 antibody heavy and light chain domains (SEQ ID NO:2 and SEQ ID NO:12 respectively).

摘要翻译： 本发明涉及雌二醇的特异性结合成员以及与其有关的材料和方法，特别是抗雌激素具有高亲和力（低解离常数）和其他类固醇的低交叉反应性的抗体或结合域。 本发明还提供了制备这样的结合成员，用于检测和/或定量雌二醇的测定方法和编码所述结合成员的核酸的方法，该核酸可用于其生产。 优选的结合成员包括具有D12抗体重链和轻链结构域的CDR区的那些（分别为SEQ ID NO：2和SEQ ID NO：12）。

公开(公告)号：US5837242A

公开(公告)日：1998-11-17

申请号：US448418

申请日：1996-05-14

申请人： Kaspar-Philipp Holliger ,  Andrew David Griffiths ,  Hendricus Renerus Jacobus Matheus Hoogenboom ,  Magnus Malmqvist ,  James David Marks ,  Brian Timothy McGuinness ,  Anthony Richard Pope ,  Terence Derek Prospero ,  Gregory Paul Winter

发明人： Kaspar-Philipp Holliger ,  Andrew David Griffiths ,  Hendricus Renerus Jacobus Matheus Hoogenboom ,  Magnus Malmqvist ,  James David Marks ,  Brian Timothy McGuinness ,  Anthony Richard Pope ,  Terence Derek Prospero ,  Gregory Paul Winter

IPC分类号： G01N33/531 ,  A61K38/00 ,  A61K39/395 ,  C07K1/00 ,  C07K2/00 ,  C07K16/00 ,  C07K16/46 ,  C07K19/00 ,  C12N1/21 ,  C12N15/09 ,  C12N15/10 ,  C12N15/113 ,  C12N15/13 ,  C12N15/62 ,  C12N15/70 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19 ,  C40B40/02

CPC分类号： C07K16/005 ,  C07K16/00 ,  C07K16/40 ,  C07K16/44 ,  C07K16/468 ,  C12N15/1037 ,  C12N15/113 ,  C40B40/02 ,  A61K38/00 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N2310/111 ,  C12N2310/1241 ,  C12N2310/127

摘要： Polypeptides comprising a first domain, which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain, which comprises a binding region of an immunoglobulin light chain variable region, the domains being linked but incapable of associating with each other to form an antigen binding site, associate to form antigen binding multimers, such as dimers, which may be multivalent or have multispecificity. The domains may be linked by a short peptide linker or may be joined directly together. Bispecific dimers may have longer linkers. Methods of preparation of the polypeptides and multimers and diverse repertoires thereof, and their display on the surface of bacteriophage for easy selection of binders of interest, are disclosed, along with many utilities.

摘要翻译： PCT No.PCT / GB93 / 02492 Sec。 371日期：1996年5月14日 102（e）日期1996年5月14日PCT提交1993年12月3日PCT公布。 公开号WO94 / 13804 日期1994年6月23日包含第一结构域的多肽，其包含免疫球蛋白重链可变区的结合区和第二结构域，其包含免疫球蛋白轻链可变区的结合区，所述结构域被连接但不能缔合 彼此形成抗原结合位点，缔合形成抗原结合多聚体，例如二聚体，其可以是多价的或具有多特异性。 结构域可以通过短肽接头连接或者可以直接连接在一起。 双特异性二聚体可以具有更长的接头。 公开了多肽和多聚体及其多种组成成分的制备方法，以及它们在噬菌体表面上的显示以便于选择感兴趣的粘合剂，以及许多实用性。