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    Process and device for the screening of molecules with regard to their individual binding behaviour towards at least one given ligand
    1.
    发明授权
    Process and device for the screening of molecules with regard to their individual binding behaviour towards at least one given ligand 失效
    关于它们对至少一个给定配体的单独结合行为的分子筛选的方法和装置

    公开(公告)号：US06713264B2

    公开(公告)日：2004-03-30

    申请号：US08891778

    申请日：1997-07-14

    申请人： Klaus Luttermann ,  Edgar Diessel ,  Winfried Kosch ,  Walter Weichel

    发明人： Klaus Luttermann ,  Edgar Diessel ,  Winfried Kosch ,  Walter Weichel

    IPC分类号： G01N3353

    CPC分类号： C12M47/04 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/0072 ,  G01N15/1468 ,  Y10S435/808

    摘要： The process is used for the screening of molecules from molecule libraries with regard to their individual binding behavior towards at least one given ligand. For this purpose the ligands labelled with a fluorescent dye are mixed with the molecule library which is in the form of a suspension. The mixture is plated out on a two-dimensional substrate (2) after the excess, unbound ligands have been washed out. Then the local fluorescence intensities on the substrate are electro-optically identified in a fluorescence microscope (5) and electronically discriminated in accordance with given selection criteria. The objects selected and localised in this way are then sequentially positioned exactly by a displacement, the coordinates of which are controlled by the image calculator, between the substrate (2) and a separation actuator (20, 21) and are spatially separated from the substrate (2) by the separation actuator (20, 21).

    摘要翻译： 该方法用于从分子文库筛选针对至少一种给定配体的单个结合行为的分子。 为此，用荧光染料标记的配体与悬浮液形式的分子文库混合。 在过量的未结合配体被洗掉之后，将混合物镀在二维底物（2）上。 然后在荧光显微镜（5）中对基底上的局部荧光强度进行电光学鉴定，并根据给定的选择标准进行电子鉴别。 以这种方式选择和定位的物体然后通过基板（2）和分离致动器（​​20,21）之间的位移（其坐标由图像计算器控制）精确地顺序地定位并且在空间上与基板分离 （2）通过分离致动器（​​20,21）。

    Method for the detection of nucleic acids
    3.
    发明申请
    Method for the detection of nucleic acids 审中-公开
    检测核酸的方法

    公开(公告)号：US20050014174A1

    公开(公告)日：2005-01-20

    申请号：US10844769

    申请日：2004-05-12

    申请人： Jens Burmeister ,  Edgar Diessel

    发明人： Jens Burmeister ,  Edgar Diessel

    IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566

    CPC分类号： C12Q1/6837 ,  C12Q1/6816 ,  C12Q2600/156 ,  C12Q2565/607 ,  C12Q2563/137 ,  C12Q2563/131 ,  C12Q2527/107 ,  C12Q2527/137

    摘要： A method is described for the specific detection of nucleic acids on a solid phase. The method involves the steps of: A) providing a surface having immobilized nucleic acids or nucleic-acid analogues, which are suitable for forming non-covalent (base-pair) bonds with the target nucleic acids, B) non-stringent hybridization of the target nucleic acids to be detected onto the immobilized nucleic acids from a solution of the analyte nucleic acid, C) labeling of the nucleic acids of the analysis mixture with labeling elements, D) optionally repeated treatment of the surface with a washing liquid in order to remove weakly bound nucleic acids, and E) detection of the nucleic-acid pairs remaining on the surface with the aid of the labeling unit bonded to them, by means of optical, optical-spectroscopic, electrical, mechanical or magnetic detection methods; wherein steps B) and C) can be carried out in any order.

    摘要翻译： 描述了用于特异性检测固相上的核酸的方法。 该方法包括以下步骤：A）提供具有固定的核酸或核酸类似物的表面，其适合于与靶核酸形成非共价键（碱基对）键，B）非严格杂交 要从分析物核酸的溶液中检测到待固定的核酸的靶核酸，C）用标记元件标记分析混合物的核酸，D）任选地用洗涤液重复处理表面，以便 去除弱结合的核酸，以及E）通过光学，光谱，电学，机械或磁性检测方法，借助于与其结合的标记单元，检测残留在表面上的核酸对; 其中步骤B）和C）可以以任何顺序进行。

    Sorting device for biological cells or viruses
    6.
    发明授权
    Sorting device for biological cells or viruses 失效
    用于生物细胞或病毒的分选装置

    公开(公告)号：US5837200A

    公开(公告)日：1998-11-17

    申请号：US653143

    申请日：1996-05-24

    申请人： Edgar Diessel ,  Walter Weichel

    发明人： Edgar Diessel ,  Walter Weichel

    IPC分类号： C12M1/00 ,  B01L3/00 ,  B03C1/035 ,  C12M1/42 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/70 ,  F15C1/08 ,  G01N15/14 ,  G01N33/569 ,  G01N33/00

    CPC分类号： F15C1/08 ,  B01L3/502761 ,  B03C1/035 ,  B01L2200/0647 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0439 ,  G01N2015/149 ,  Y10S209/906 ,  Y10S435/808

    摘要： The sorting device is able to sort biological objects, particularly cells or viruses, in liquids as a function of physically measurable criteria or properties. The sorting apparatus is provided with a feed inlet (1) for the biological liquid which branches into a microstructured system of multi-parallel main channels (3) and sorting modules (4) each with a switch unit for distribution to two different outlet channels (19,20). Inside a sorting module (4), at least one sensor (9,10) is arranged on each main channel, and a sorting actuator (12) controlled by the relevant sensor is arranged on each switch unit (11). Finally, each outlet channel (20) is connected to a collecting channel (6) for the unselected biological cells or viruses, and each outlet channel (19) is connected to a collecting channel (5) for the selected cells or viruses. If the cells or viruses are magnetically marked, it is possible to dispense with the sensor (9,10) on the main channels (3). In that case, the sorting actuators arranged on the switch units are designed as magnetic deflection modules (37,38). In this case, the magnetic deflection modules undertake direct classification or selection as a function of the magnetic properties of the biological objects to be sorted.

    摘要翻译： 分选装置能够根据物理上可测量的标准或性质将生物体，特别是细胞或病毒分离在液体中。 分拣装置设置有用于生物液体的进料口（1），其分支成多平行主通道（3）和分拣模块（4）的微结构化系统，每个分配模块具有开关单元，用于分配到两个不同的出口通道 19,20）。 在分拣模块（4）内，在每个主通道上布置有至少一个传感器（9,10），并且在每个开关单元（11）上布置由相关传感器控制的分拣机构（12）。 最后，每个出口通道（20）连接到用于未选择的生物细胞或病毒的收集通道（6），并且每个出口通道（19）连接到用于所选细胞或病毒的收集通道（5）。 如果细胞或病毒被磁性标记，则可以省去主通道（3）上的传感器（9,10）。 在这种情况下，布置在开关单元上的排序致动器被设计为磁偏转模块（37,38）。 在这种情况下，磁偏转模块根据要分选的生物体的磁特性进行直接分类或选择。

    Method and device for the quantitative electrical detection of analytes
    7.
    发明申请
    Method and device for the quantitative electrical detection of analytes 审中-公开
    分析物定量电检测的方法和装置

    公开(公告)号：US20050112617A1

    公开(公告)日：2005-05-26

    申请号：US10870190

    申请日：2004-06-16

    申请人： Edgar Diessel ,  Jens Burmeister

    发明人： Edgar Diessel ,  Jens Burmeister

    IPC分类号： G01N33/53 ,  C12Q1/68 ,  G01N27/06 ,  G01N27/12 ,  G01N33/543 ,  G01N37/00

    CPC分类号： G01N33/5438 ,  C12Q1/6816 ,  G01N27/12 ,  C12Q2565/607

    摘要： The invention relates to a method for detecting one or more analytes by a recognition reaction, using a device with an electrolyte-filled electrolyte space, having at least one measurement electrode, optionally with an insulating region (insulator layer) next to the measurement electrode, and recognition molecules which are immobilized on the measurement electrode and/or on the insulating region next to the measurement electrode, and a counterelectrode. The electrical conductance or the resistance between the measurement electrode/s and the counterelectrode is measured as a function of the development time, and the time profile of the conductance or the resistance is analyzed. The invention also relates to a device with which the method according to the invention can be carried out.

    摘要翻译： 本发明涉及一种通过识别反应检测一种或多种分析物的方法，该装置使用具有电解质填充的电解质空间的装置，该装置具有至少一个测量电极，可选地具有邻近测量电极的绝缘区域（绝缘体层） 以及固定在测量电极上和/或在测量电极旁边的绝缘区域上的识别分子和反电极。 作为显影时间的函数测量测量电极和对电极之间的电导率或电阻，并分析电导或电阻的时间曲线。 本发明还涉及可以执行根据本发明的方法的装置。

    Method for determining an active agent dose
    8.
    发明申请
    Method for determining an active agent dose 审中-公开
    确定活性剂剂量的方法

    公开(公告)号：US20050074803A1

    公开(公告)日：2005-04-07

    申请号：US10947982

    申请日：2004-09-23

    申请人： Walter Schmitt ,  Stefan Willmann ,  Edgar Diessel ,  Ingmar Dorn ,  Jens Burmeister

    发明人： Walter Schmitt ,  Stefan Willmann ,  Edgar Diessel ,  Ingmar Dorn ,  Jens Burmeister

    IPC分类号： G06F19/18 ,  G06Q50/24 ,  C12Q1/68 ,  G06F17/60 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50

    CPC分类号： G06Q50/24 ,  G16B20/00

    摘要： Method for determining the dose of at least one active agent based on a genetic analysis. The method comprises analyzing specific genes for their nucleotide sequence or their expression levels of gene-specific proteins and/or RNA molecules. The gene-specific data is assigned one or more relevant physiological functions of the human or animal body, in particular those which have an influence on the metabolism, absorption, excretion or distribution of the active agent in the body. The gene-specific data and the assigned physiological functions are then integrated into a physiology-based pharmacokinetic model (PBPK model). The PBPK model integrates pharmacokinetic data relating to one or more active agents. The PBPK model may also receive and evaluate patient-specific data directly inputted and combined with data from a knowledge database comprising known values of pharmacokinetic parameters. The PBPK's integration of these data provide a calculation of the individual dose of the active agent.

    摘要翻译： 基于遗传分析确定至少一种活性剂的剂量的方法。 该方法包括分析其核苷酸序列的特定基因或其基因特异性蛋白质和/或RNA分子的表达水平。 基因特异性数据被分配人或动物体的一个或多个相关的生理功能，特别是那些对体内活性剂的代谢，吸收，排泄或分布有影响的那些。 然后将基因特异性数据和分配的生理功能整合到基于生理学的药代动力学模型（PBPK模型）中。 PBPK模型整合了与一种或多种活性剂有关的药代动力学数据。 PBPK模型还可以接收和评估直接输入的患者特异性数据，并与包括已知的药代动力学参数值的知识数据库的数据组合。 PBPK对这些数据的整合提供了活性剂个体剂量的计算。

    Device for separating micro objects
    9.
    发明授权
    Device for separating micro objects 失效
    用于分离微物体的装置

    公开(公告)号：US06517779B1

    公开(公告)日：2003-02-11

    申请号：US09214839

    申请日：1999-01-13

    申请人： Klaus Luttermann ,  Edgar Diessel ,  Markus Weidauer

    发明人： Klaus Luttermann ,  Edgar Diessel ,  Markus Weidauer

    IPC分类号： B01L302

    CPC分类号： C12M47/04 ,  B01L3/021 ,  C12M35/00

    摘要： The device serves for the transfer of microobjects, in particular of biological objects, from a current substrate (9) to a target substrate. In this case, use is made of a displaceable pipette system which is connected to a pressure generating device for aspirating in and flushing out the biological objects. The pipette system is disposed in a micromanipulator (3) and consists mainly of a substantially vertically disposed microcapillary (1) having a clear width of 1 &mgr;m to 50 &mgr;m.

    摘要翻译： 该装置用于将微物体，特别是生物物体从当前基底（9）转移到目标基底。 在这种情况下，使用可移位移液管系统，其连接到用于吸入和冲洗生物体的压力产生装置。 移液管系统设置在显微操纵器（3）中，主要由基本上垂直设置的微毛细管（1）组成，微毛细管（1）的清晰宽度为1〜50μm。

    Semiconductor component with adiabatic transport in edge channels
    10.
    发明授权
    Semiconductor component with adiabatic transport in edge channels 失效
    边缘通道绝热运输的半导体元件

    公开(公告)号：US5332911A

    公开(公告)日：1994-07-26

    申请号：US822299

    申请日：1992-01-17

    申请人： Klaus von Klitzing ,  Gerhard Mueller ,  Edgar Diessel ,  Dieter Weiss

    发明人： Klaus von Klitzing ,  Gerhard Mueller ,  Edgar Diessel ,  Dieter Weiss

    IPC分类号： G01J1/02 ,  G01T1/24 ,  H01L31/0352 ,  H01L31/08 ,  H01L37/00 ,  H01L27/14 ,  H01L31/00

    CPC分类号： H01L31/0352 ,  H01L31/08

    摘要： A semiconductor radiation detector with layer-wise construction and a contive region having a two-dimensional or quasi one-dimensional electron or hole gas is provided in which adiabatic transport in edge channels occurs at least regionally and in which at least two contacts are provided to this conductive region. The transport in the edge channels is disturbed by interaction with the electromagnetic radiation to be detected, i.e. an increase of the scattering rate between the edge channels is caused. This leads to a change of the resistance measurable between the contacts, with a means being provided for measuring the change of resistance to thereby detect the incident radiation.

    摘要翻译： 提供具有层次构造的半导体辐射检测器和具有二维或准一维电子或空穴气体的导电区域，其中边缘通道中的绝热传输至少在区域上发生，并且其中至少两个触点被提供到 这个导电区域。 通过与待检测的电磁辐射的相互作用扰乱边缘通道的传输，即引起边缘通道之间的散射速率的增加。 这导致在触点之间可测量的电阻的变化，提供了用于测量电阻变化从而检测入射辐射的装置。