Soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene
    6.
    发明授权
    Soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene 有权
    土壤微生物,从土壤微生物分离的新型氧化还原酶,编码氧化还原酶的基因,以及使用微生物,氧化还原酶和基因产生糖苷配基的方法

    公开(公告)号:US09394562B2

    公开(公告)日:2016-07-19

    申请号:US14588790

    申请日:2015-01-02

    Abstract: The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.

    Abstract translation: 本发明涉及新根瘤菌属(Rhizobium sp。) GIN611KCTC11708BP或其细胞提取物,对具有糖酵解活性的新型氧化还原酶,与编码氧化还原酶的基因相连,包含重组载体蛋白的重组菌株或编码重组蛋白的表达载体,以及糖酵解方法 使用与生物催化剂相同的天然产物。 本发明还涉及使用它们的各种天然产物制备糖苷配基的方法。 从本发明的新型微生物分离的新型氧化还原酶不属于葡萄糖苷酶基团,属于氧化还原酶基团,对天然产物具有糖酵解活性。 新型氧化还原酶氧化天然产物的糖苷配基中的糖,从而产生各种糖苷配基。

    POSITIVE AND NEGATIVE EXTRACTION DEVICE AND EXTRACTION METHOD
    10.
    发明申请
    POSITIVE AND NEGATIVE EXTRACTION DEVICE AND EXTRACTION METHOD 审中-公开
    积极和负面提取装置和提取方法

    公开(公告)号:US20160016984A1

    公开(公告)日:2016-01-21

    申请号:US14807971

    申请日:2015-07-24

    CPC classification number: B01D11/0215 C07D407/04

    Abstract: Provided is a pressure-cycling type of extraction device comprising: an extraction unit for accommodating and extracting an extraction target substance; a compression unit for increasing the pressure inside the extraction unit; and a decompression unit for decreasing the pressure inside the extraction unit; wherein the pressure inside the extraction unit is increased and decreased by alternately operating the compression unit and the decompression unit, and the extraction unit comprises a bubbling nozzle for supplying bubbles into the extraction target substance. Further provided is a pressure-cycling type of extraction method in which a compression process and a decompression process are carried out alternately, and compositions produced by the method. The extraction device and method allow low temperature extraction, permit outstanding extraction efficiency and can prevent colour changes, olfactory changes and thermal denaturation of the extraction target substance.

    Abstract translation: 提供一种压力循环型提取装置,包括:提取单元,用于容纳和提取提取目标物质; 用于增加提取单元内的压力的压缩单元; 以及用于降低提取单元内的压力的减压单元; 其中,通过交替地操作压缩单元和减压单元,提取单元内的压力增加和减少,并且提取单元包括用于向提取目标物质中提供气泡的鼓泡喷嘴。 进一步提供了一种压力循环型提取方法,其中交替进行压缩过程和减压过程,以及通过该方法制备的组合物。 提取装置和方法允许低温提取,提供优异的提取效率,并可以防止提取目标物质的颜色变化,嗅觉变化和热变性。

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