公开(公告)号：US20220155251A1

公开(公告)日：2022-05-19

申请号：US17649842

申请日：2022-02-03

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N27/26 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  B01F33/302 ,  B01F33/3031 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/327 ,  G01N27/416

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

公开(公告)号：US20150314293A1

公开(公告)日：2015-11-05

申请号：US14719731

申请日：2015-05-22

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: B01L3/00 ,  G01N27/447

CPC classification number: G01N33/54326 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0427 ,  B01L2400/043 ,  C12M25/01 ,  C12M25/16 ,  G01N27/44717 ,  G01N27/44786 ,  G01N33/54386 ,  G01N35/0098 ,  G01N2035/1046

Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

Abstract translation: 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器，其包括：（i）包括布置用于在表面上进行液滴操作的电极的基板; （ii）起始液滴，包括：（1）一个或多个磁响应珠; （2）物质的起始量; 和（3）起始体积; （b）将一个或多个磁响应珠磁性地固定在距离目标液滴分裂区一定距离的位置; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分开所述组合的液滴以产生一组液滴，所述液滴包括：（i）包含基本上所有所述一个或多个磁响应珠粒并且具有减少量 物质相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴。

公开(公告)号：US20150174577A1

公开(公告)日：2015-06-25

申请号：US14639612

申请日：2015-03-05

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01L3/00 ,  B03C5/02 ,  B81B1/00

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0448 ,  B03C5/02 ,  B81B1/00 ,  G01N27/447 ,  G01N2035/1046

Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.

Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了液滴微致动器，其包括：（a）第一基板，其包括被配置用于在所述基板的表面上进行液滴操作的电极; （b）第二衬底，其与衬底的表面间隔足以限定第一衬底和第二衬底之间的内部空间的距离，其中所述距离足以容纳设置在第一衬底上的空间中的液滴; 和（c）布置在内部体积中并且以允许使用电极对液滴进行液滴操作的方式相对于电极布置的液滴。

公开(公告)号：US20170080428A1

公开(公告)日：2017-03-23

申请号：US15367046

申请日：2016-12-01

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Alexander D. Shenderov ,  Michael G. Pollack

IPC: B01L7/00

CPC classification number: B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.

公开(公告)号：US09476856B2

公开(公告)日：2016-10-25

申请号：US13890715

申请日：2013-05-09

Applicant: Advanced Liquid Logic Inc. ,  Duke University

Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N33/543 ,  G01N27/72 ,  G01N27/74

CPC classification number: G01N27/72 ,  G01N27/745 ,  G01N33/54326

Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.

Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。

公开(公告)号：US20190324026A1

公开(公告)日：2019-10-24

申请号：US16455506

申请日：2019-06-27

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: G01N33/543 ,  B01L3/00 ,  G01N27/447 ,  C12M1/12

Abstract: A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.

公开(公告)号：US12181467B2

公开(公告)日：2024-12-31

申请号：US16455506

申请日：2019-06-27

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/12 ,  G01N27/447 ,  G01N35/00 ,  G01N35/10

Abstract: A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.

公开(公告)号：US11255809B2

公开(公告)日：2022-02-22

申请号：US16788187

申请日：2020-02-11

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N27/26 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/327 ,  G01N27/416 ,  B01L3/02

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

公开(公告)号：US10379112B2

公开(公告)日：2019-08-13

申请号：US14719731

申请日：2015-05-22

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/543 ,  C12M1/12 ,  G01N35/10 ,  G01N35/00

Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

公开(公告)号：US09952177B2

公开(公告)日：2018-04-24

申请号：US14795304

申请日：2015-07-09

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

IPC: G01N27/447 ,  G01N27/453 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N27/44743 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/046 ,  G01N27/44791

Abstract: The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.