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    Droplet-based affinity assays
    1.
    发明授权
    Droplet-based affinity assays 有权
    基于液滴的亲和力测定

    公开(公告)号：US09476856B2

    公开(公告)日：2016-10-25

    申请号：US13890715

    申请日：2013-05-09

    Applicant: Advanced Liquid Logic Inc. ,  Duke University

    Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

    IPC: G01N33/543 ,  G01N27/72 ,  G01N27/74

    CPC classification number: G01N27/72 ,  G01N27/745 ,  G01N33/54326

    Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.

    Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。

    Droplet Actuator Devices and Methods Employing Magnetic Beads
    3.
    发明申请
    Droplet Actuator Devices and Methods Employing Magnetic Beads 审中-公开
    使用磁珠的液滴致动器装置和方法

    公开(公告)号：US20150314293A1

    公开(公告)日：2015-11-05

    申请号：US14719731

    申请日：2015-05-22

    Applicant: Advanced Liquid Logic, Inc. ,  Duke University

    Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

    IPC: B01L3/00 ,  G01N27/447

    CPC classification number: G01N33/54326 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0427 ,  B01L2400/043 ,  C12M25/01 ,  C12M25/16 ,  G01N27/44717 ,  G01N27/44786 ,  G01N33/54386 ,  G01N35/0098 ,  G01N2035/1046

    Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

    Abstract translation: 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器，其包括：（i）包括布置用于在表面上进行液滴操作的电极的基板; （ii）起始液滴，包括：（1）一个或多个磁响应珠; （2）物质的起始量; 和（3）起始体积; （b）将一个或多个磁响应珠磁性地固定在距离目标液滴分裂区一定距离的位置; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分开所述组合的液滴以产生一组液滴，所述液滴包括：（i）包含基本上所有所述一个或多个磁响应珠粒并且具有减少量 物质相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴。

    Droplet actuator devices and methods employing magnetic beads
    7.
    发明授权
    Droplet actuator devices and methods employing magnetic beads 有权

    公开(公告)号：US10379112B2

    公开(公告)日：2019-08-13

    申请号：US14719731

    申请日：2015-05-22

    Applicant: Advanced Liquid Logic, Inc. ,  Duke University

    Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

    IPC: B01L3/00 ,  G01N27/447 ,  G01N33/543 ,  C12M1/12 ,  G01N35/10 ,  G01N35/00

    Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

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