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    METHOD FOR IMPROVED PRODUCTION OF INTRACELLULAR PROTEINS IN BACILLUS
    1.
    发明申请
    METHOD FOR IMPROVED PRODUCTION OF INTRACELLULAR PROTEINS IN BACILLUS 有权

    公开(公告)号：US20250051822A1

    公开(公告)日：2025-02-13

    申请号：US18720123

    申请日：2022-12-19

    Applicant: BASF SE

    Inventor： Max Fabian Felle ,  Christopher Sauer ,  Mathis Appelbaum ,  Stefan Jenewein ,  Sebastian Schoof ,  Christian Riedele

    IPC: C12P21/02 ,  C12N9/38 ,  C12N15/75

    Abstract: The present invention relates to a method for producing at least one polypeptide of interest in a Bacillus host cell, said method comprising the steps of a) providing a Bacillus host cell comprising a polynucleotide encoding the at least one polypeptide of interest, b) cultivating the Bacillus host cell under conditions which allow for intracellular accumulation of said at least one polypeptide of interest, thereby producing said at least one polypeptide of interest, c) stop of aeration and addition of feed, and d) incubating the cells for at least hours. Moreover, the present invention relates to a Bacillus host cell comprising a polynucleotide encoding at least one polypeptide of interest lacking a functional secretion sequence, wherein the ispA gene has been inactivated in the Bacillus host cell.

    ALANINE RACEMASE DOUBLE DELETION AND TRANSCOMPLEMENTATION
    3.
    发明公开
    ALANINE RACEMASE DOUBLE DELETION AND TRANSCOMPLEMENTATION 审中-公开

    公开(公告)号：US20230295603A1

    公开(公告)日：2023-09-21

    申请号：US18017430

    申请日：2021-07-23

    Applicant: BASF SE

    Inventor： Max Fabian Felle ,  Stefan Jenewein ,  Christopher Sauer ,  Tobias Klein

    IPC: C12N9/90 ,  C12N15/75

    CPC classification number: C12N9/90 ,  C12N15/75 ,  C12Y501/01001 ,  C12N2800/101

    Abstract: The present invention relates to a bacterial host cell in which a first chromosomal gene encoding a first alanine racemase and a second chromosomal gene encoding a second alanine racemase have been inactivated. Said bacterial host cell comprises – either on a plasmid comprising at least one autonomous replication sequence or present as multiple copies in the chromosome – a gene expression cassette comprising a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter, and a polynucleotide encoding a third alanine racemase, operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

    AMYLASE ENZYMES
    4.
    发明申请
    AMYLASE ENZYMES 有权

    公开(公告)号：US20220162576A1

    公开(公告)日：2022-05-26

    申请号：US17442489

    申请日：2020-03-24

    Applicant: BASF SE

    Inventor： Stefan Jenewein ,  Zachary David Miles ,  Asfia Qureshi ,  Jared Dennis ,  Tong Li ,  Xuqiu Tan ,  Priya Anand ,  Jesper Nielsen

    IPC: C12N9/28

    Abstract: Genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. Fragments of parent amylase enzymes activity and methods of using the fragments for making genetically engineered enzymes having amylase activity. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.

    Improved Combination of Protease and Protease Inhibitor with Secondary Enzyme
    6.
    发明公开
    Improved Combination of Protease and Protease Inhibitor with Secondary Enzyme 审中-公开

    公开(公告)号：US20240240114A1

    公开(公告)日：2024-07-18

    申请号：US18027461

    申请日：2021-09-17

    Applicant: BASF SE

    Inventor： Stefan Jenewein ,  Gabriele Boenemann

    IPC: C11D3/386 ,  C11D3/32 ,  C12N9/54

    CPC classification number: C11D3/386 ,  C11D3/32 ,  C12N9/54

    Abstract: The present invention relates to a composition comprising an improved combination of protease and protease inhibitor. It was revealed that the combination of a protease with introduced negative charges in the active site loop and a peptide aldehyde-based protease inhibitor results in an increased stability of additional enzymes being also present in a composition together with the protease and the peptide aldehyde-based protease inhibitor. This is in particular beneficial for detergent compositions comprising proteases together with other enzymes and which frequently suffer from enzyme instability during storage.

    ALANINE RACEMASE SINGLE DELETION AND TRANSCOMPLEMENTATION
    8.
    发明公开
    ALANINE RACEMASE SINGLE DELETION AND TRANSCOMPLEMENTATION 审中-公开

    公开(公告)号：US20230272358A1

    公开(公告)日：2023-08-31

    申请号：US18017426

    申请日：2021-07-23

    Applicant: BASF SE

    Inventor： Stefan Jenewein ,  Max Fabian Felle ,  Christopher Sauer ,  Tobias Klein

    IPC: C12N9/48 ,  C12N15/75 ,  C12N9/90 ,  C12N1/20

    CPC classification number: C12N9/48 ,  C12N15/75 ,  C12N9/90 ,  C12N1/20 ,  C12R2001/10

    Abstract: The present invention relates to a Bacillus host cell belonging to the species Bacillus lichemformis or Bacillus pumilus in which the chromosomal alr gene has been inactivated. Said bacterial host cell comprises a plasmid comprising at least one autonomous replication sequence, a first polynucleotide encoding at least one polypeptide of interest, wherein said first polynucleotide is operably linked to a promoter, and a second polynucleotide encoding an alanine racemase which is not native to the host cell, wherein said second polynucleotide is operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

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