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公开(公告)号：US07829316B2
公开(公告)日：2010-11-09
申请号：US12104595
申请日：2008-04-17
申请人： Chie Koseki , Keita Fukui , Jun Nakamura , Hiroyuki Kojima
发明人： Chie Koseki , Keita Fukui , Jun Nakamura , Hiroyuki Kojima
CPC分类号： C12P7/46
摘要： Disclosed is a process for production of succinic acid, which comprises the step of reacting a bacterium which has been modified so as to increase the expression of a sucE1 gene or a product produced by any treatment of the bacterium with an organic raw material in a reaction solution containing a carbonate ion, a bicarbonate ion or carbon dioxide gas to thereby yield the desired succinic acid.
摘要翻译： 公开了一种生产琥珀酸的方法，其包括使已经被修饰以增加sucE1基因的表达的细菌或通过任何处理细菌产生的产物与反应中的有机原料反应的步骤 含有碳酸根离子，碳酸氢根离子或二氧化碳气体的溶液，从而得到所需的琥珀酸。
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公开(公告)号：US07993888B2
公开(公告)日：2011-08-09
申请号：US12280426
申请日：2007-02-23
申请人： Makoto Murase , Ryusuke Aoyama , Akiko Sakamoto , Sanae Sato , Madoka Yonekura , Shuichi Yunomura , Kenji Yamagishi , Keita Fukui , Chie Koseki , Jun Nakamura , Hiroyuki Kojima
发明人： Makoto Murase , Ryusuke Aoyama , Akiko Sakamoto , Sanae Sato , Madoka Yonekura , Shuichi Yunomura , Kenji Yamagishi , Keita Fukui , Chie Koseki , Jun Nakamura , Hiroyuki Kojima
CPC分类号： C12P7/46
摘要： Provided is a bacterium which is capable of producing an organic acid and is modified so as to have an enhanced 2-oxoglutarate dehydrogenase activity as compared with that of an unmodified strain. An organic acid such as succinic acid can be produced by culturing the bacterium.
摘要翻译： 本发明提供一种能够产生有机酸的细菌，并且与未改变的菌株相比被改性以具有增强的2-氧戊二酸脱氢酶活性。 可以通过培养细菌来生产琥珀酸等有机酸。
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3.发明申请公开(公告)号：US20090156779A1
公开(公告)日：2009-06-18
申请号：US12280426
申请日：2007-02-23
申请人： Makoto Murase , Ryusuke Aoyama , Akiko Sakamoto , Sanae Sato , Madoka Yonekura , Shuichi Yunomura , Kenji Yamagishi , Keita Fukui , Chie Koseki , Jun Nakamura , Hiroyuki Kojima
发明人： Makoto Murase , Ryusuke Aoyama , Akiko Sakamoto , Sanae Sato , Madoka Yonekura , Shuichi Yunomura , Kenji Yamagishi , Keita Fukui , Chie Koseki , Jun Nakamura , Hiroyuki Kojima
CPC分类号： C12P7/46
摘要： Provided is a bacterium which is capable of producing an organic acid and is modified so as to have an enhanced 2-oxoglutarate dehydrogenase activity as compared with that of an unmodified strain. An organic acid such as succinic acid can be produced by culturing the bacterium.
摘要翻译： 本发明提供一种能够产生有机酸的细菌，并且与未改变的菌株相比被改性以具有增强的2-氧戊二酸脱氢酶活性。 可以通过培养细菌来生产琥珀酸等有机酸。
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4.发明授权公开(公告)号：US08628941B2
公开(公告)日：2014-01-14
申请号：US11753710
申请日：2007-05-25
申请人： Keita Fukui , Jun Nakamura , Hiroyuki Kojima
发明人： Keita Fukui , Jun Nakamura , Hiroyuki Kojima
IPC分类号： C12P13/14
摘要： Coryneform bacteria are described that have an ability to produce L-amino acids and are modified so that acetyl-CoA hydrolase activity is decreased. The bacteria are used to produce L-amino acids generated by a biosynthetic pathway in which pyruvic acid is an intermediate, such as L-glutamic acid, L-arginine, L-glutamine, L-proline, L-alanine, L-valine, and L-lysine.
摘要翻译： 描述了具有产生L-氨基酸并且被修饰以使乙酰辅酶A水解酶活性降低的能力的棒状细菌。 细菌用于产生由丙酮酸是L-谷氨酸，L-精氨酸，L-谷氨酰胺，L-脯氨酸，L-丙氨酸，L-缬氨酸等中间体的生物合成途径产生的L-氨基酸， 和L-赖氨酸。
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5.发明授权公开(公告)号：US07972823B2
公开(公告)日：2011-07-05
申请号：US11561011
申请日：2006-11-17
申请人： Keita Fukui , Jun Nakamura , Hiroyuki Kojima
发明人： Keita Fukui , Jun Nakamura , Hiroyuki Kojima
CPC分类号： C12P7/46 , C08G63/16 , C12N9/16 , C12N15/77 , C12Y301/02001
摘要： Coryneform bacterium is modified so that an activity of acetyl-CoA hydrolase is decreased, and succinic acid is produced by using the bacterium.
摘要翻译： 修饰棒状细菌使得乙酰辅酶A水解酶的活性降低，并且通过使用细菌产生琥珀酸。
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6.发明申请公开(公告)号：US20070254345A1
公开(公告)日：2007-11-01
申请号：US11753710
申请日：2007-05-25
申请人： Keita Fukui , Jun Nakamura , Hiroyuki Kojima
发明人： Keita Fukui , Jun Nakamura , Hiroyuki Kojima
摘要： Coryneform bacteria are described that have an ability to produce L-amino acids and are modified so that acetyl-CoA hydrolase activity is decreased. The bacteria are used to produce L-amino acids generated by a biosynthetic pathway in which pyruvic acid is an intermediate, such as L-glutamic acid, L-arginine, L-glutamine, L-proline, L-alanine, L-valine, and L-lysine.
摘要翻译： 描述了具有产生L-氨基酸并且被修饰以使乙酰辅酶A水解酶活性降低的能力的棒状细菌。 细菌用于产生由丙酮酸是L-谷氨酸，L-精氨酸，L-谷氨酰胺，L-脯氨酸，L-丙氨酸，L-缬氨酸等中间体的生物合成途径产生的L-氨基酸， 和L-赖氨酸。
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公开(公告)号：US20060205048A1
公开(公告)日：2006-09-14
申请号：US11362931
申请日：2006-02-28
申请人： Makoto Murase , Ryusuke Aoyama , Miki Ikuta , Kenji Yamagishi , Mika Moriya , Jun Nakamura , Hiroyuki Kojima
发明人： Makoto Murase , Ryusuke Aoyama , Miki Ikuta , Kenji Yamagishi , Mika Moriya , Jun Nakamura , Hiroyuki Kojima
CPC分类号： C12P7/46
摘要： Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.
摘要翻译： 琥珀酸是通过使经修饰的细菌增强富马酸盐还原酶活性或其制备方法与含有碳酸根离子，碳酸氢根离子和二氧化碳气体的反应溶液中的有机原料反应生成琥珀酸而制备的。 更优选地，通过使经修饰的细菌增强富马酸盐还原酶和丙酮酸羧化酶的活性并降低乳酸脱氢酶活性或其细胞制备，在含有碳酸根离子，碳酸氢盐 离子和二氧化碳气体产生琥珀酸。 通过收集产生的琥珀酸获得琥珀酸。
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8.发明授权Method of producing succinic acid with bacterium comprising a modified fumarate reductase gene or a modified succinate dehydrogenase gene 有权用包含改性的富马酸酯还原酶基因或修饰的琥珀酸脱氢酶基因的细菌生产琥珀酸的方法公开(公告)号：US07763447B2
公开(公告)日：2010-07-27
申请号：US11362931
申请日：2006-02-28
申请人： Makoto Murase , Ryusuke Aoyama , Miki Ikuta , Kenji Yamagishi , Mika Moriya , Jun Nakamura , Hiroyuki Kojima
发明人： Makoto Murase , Ryusuke Aoyama , Miki Ikuta , Kenji Yamagishi , Mika Moriya , Jun Nakamura , Hiroyuki Kojima
CPC分类号： C12P7/46
摘要： Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.
摘要翻译： 琥珀酸是通过使经修饰的细菌增强富马酸盐还原酶活性或其制备方法与含有碳酸根离子，碳酸氢根离子和二氧化碳气体的反应溶液中的有机原料反应生成琥珀酸而制备的。 更优选地，通过使经修饰的细菌增强富马酸盐还原酶和丙酮酸羧化酶的活性并降低乳酸脱氢酶活性或其细胞制备，在含有碳酸根离子，碳酸氢盐 离子和二氧化碳气体产生琥珀酸。 通过收集产生的琥珀酸获得琥珀酸。
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公开(公告)号：US10266909B2
公开(公告)日：2019-04-23
申请号：US14007992
申请日：2012-03-19
申请人： Tomohiko Omura , Jun Nakamura , Hirokazu Okada , Hiroyuki Semba , Yusaku Tomio , Hiroyuki Hirata , Masaaki Igarashi , Kazuhiro Ogawa , Masaaki Terunuma
发明人： Tomohiko Omura , Jun Nakamura , Hirokazu Okada , Hiroyuki Semba , Yusaku Tomio , Hiroyuki Hirata , Masaaki Igarashi , Kazuhiro Ogawa , Masaaki Terunuma
IPC分类号： C21D9/46 , C22C38/00 , C22C38/02 , C22C38/06 , C22C38/42 , C22C38/44 , C22C38/46 , C22C38/48 , C22C38/50 , C22C38/58 , C22C38/52 , C22C38/54 , C21D6/00 , C21D8/02 , C21D1/26 , F16L9/02
摘要： There is provided an austenitic stainless steel for high-pressure hydrogen gas consisting, by mass percent, of C: 0.10% or less, Si: 1.0% or less, Mn: 3% or more to less than 7%, Cr: 15 to 30%, Ni: 10% or more to less than 17%, Al: 0.10% or less, N: 0.10 to 0.50%, and at least one kind of V: 0.01 to 1.0% and Nb: 0.01 to 0.50%, the balance being Fe and impurities, wherein in the impurities, the P content is 0.050% or less and the S content is 0.050% or less, the tensile strength is 800 MPa or higher, the grain size number (ASTM E112) is No. 8 or higher, and alloy carbo-nitrides having a maximum diameter of 50 to 1000 nm are contained in the number of 0.4/μm2 or larger in cross section observation.
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公开(公告)号：US09929386B2
公开(公告)日：2018-03-27
申请号：US13318328
申请日：2010-05-13
IPC分类号： H01M2/10 , H01M10/04 , H01M10/48 , H01M10/625 , H01M10/6563 , H01M10/647 , H01M10/6557 , H01M10/613 , H01M10/6562
CPC分类号： H01M2/1077 , H01M10/0481 , H01M10/486 , H01M10/613 , H01M10/625 , H01M10/647 , H01M10/6557 , H01M10/6562 , H01M10/6563
摘要： There is provided a battery assembly wherein assembly can be facilitated by reducing the number of components to be assembled, by integrally fixing resin plates (52, 62) on the inside of metal plates (51, 61) of end plates (5, 6). A laminated battery body (3) is constituted by adjacently arranging battery holders (2) made of resin forming cooling passages (4) between a plurality of batteries (1) and holding such batteries (1) on both sides between other batteries (1). The metal plates (51, 61) and resin plates (52, 62) that are integrally fixed on the inside of these metal plates are provided on the outside of the batteries (1) at both ends of the laminated battery body (3). Thus, the batteries (1) are held by the resin plates (52, 62), and the end plates (5, 6) are respectively arranged to form the cooling passages (4) between these batteries (1) and the metal plates (51, 61). The laminated battery body (3) is fixed in a clamped manner by fixing means clamping these end plates (5, 6).
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