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公开(公告)号:US10577611B2
公开(公告)日:2020-03-03
申请号:US14249533
申请日:2014-04-10
申请人: DSM IP ASSETS B.V.
摘要: The present invention relates to polynucleotide sequences which enable a polynucleotide control sequence, such as a promoter, to direct expression in a wide range of industrially relevant species, both prokaryotes and eukaryotes. When the polynucleotide sequences of the invention are applied in combination with selection marker genes it is possible to perform selectable cloning in a laboratory host and use the same construct in the final host.
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公开(公告)号:US10472661B2
公开(公告)日:2019-11-12
申请号:US15516854
申请日:2015-10-08
申请人: DSM IP ASSETS B.V.
摘要: The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises: a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.
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公开(公告)号:US20150031868A1
公开(公告)日:2015-01-29
申请号:US14373571
申请日:2013-01-23
申请人: DSM IP ASSETS B.V.
发明人: Martin Lehmann , Joshua Trueheart , Priscilla Zwart Jens , Liang Wu , Viktor Marius Boer , Cornelis Maria Jacobus Sagt , Manoj Kumar , Bernard Meijrink , Marco Alexander Van Den Berg
IPC分类号: C12P19/56 , C12N9/10 , A23L1/236 , C07H15/256
CPC分类号: C12P19/56 , A23K20/10 , A23K20/121 , A23K20/163 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0073 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/007 , C12Y114/13078 , C12Y204/01035 , C12Y402/03019 , C12Y505/01013
摘要: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.
摘要翻译: 本发明涉及包含一个或多个核苷酸序列的重组微生物,所述核苷酸序列编码:具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有海藻酸13-羟化酶活性的多肽,由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。 重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶,使得微生物能够产生一种或多种甜菊糖苷。
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公开(公告)号:US20140178932A1
公开(公告)日:2014-06-26
申请号:US14192236
申请日:2014-02-27
申请人: DSM IP ASSETS B.V.
IPC分类号: C12N15/80
摘要: The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.
摘要翻译: 本发明提供丝状真菌细胞的转染方法,包括提供多个容器,向每个容器填充一定量的转染所需的聚合物,将待转染的细胞以及转染试剂的水溶液加入 每个容器,孵育所得混合物,从培养的混合物中除去转染试剂; 并选择已经转化的细胞,其特征在于培养混合物的总体积小于每个容器1ml。 此外,本发明提供了转化的丝状真菌细胞用于生产蛋白质或代谢物的用途。
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公开(公告)号:US12114682B2
公开(公告)日:2024-10-15
申请号:US15734094
申请日:2019-06-03
申请人: DSM IP ASSETS B.V.
摘要: The present invention describes a process for modifying a sweet protein or a taste modifying protein, comprising incubating a sweet protein solution or a taste modifying protein solution with a peptidyl arginine deiminase (PAD).
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公开(公告)号:US11627752B2
公开(公告)日:2023-04-18
申请号:US17689765
申请日:2022-03-08
申请人: DSM IP Assets B.V.
IPC分类号: A23L27/21 , A23L9/10 , A23C9/156 , A23L2/56 , A23L9/20 , A23L33/14 , A23L23/00 , A23L23/10 , A23L27/00 , A23L31/15 , A23L27/23 , A23L2/60 , C12N9/24
摘要: The present invention relates to a method for producing a flavour composition comprising providing a slurry of yeast cell walls and contacting the slurry of yeast cell walls with a glucanase and with an endoprotease, followed by separating a liquid fraction by solid/liquid separation to provide the liquid flavour composition.
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公开(公告)号:US11020423B2
公开(公告)日:2021-06-01
申请号:US16696637
申请日:2019-11-26
申请人: DSM IP ASSETS B.V.
发明人: Hendrik Arie Schols , Paulus De Vos , Marco Alexander Van Den Berg , Geert Bruggeman , Erik Martinus Andrianus Maria Bruininx , Neha Mohan Sahasrabudhe , Jan Scholte , Lingmin Tian , Anton Johannes Scheurink
IPC分类号: A61K31/732 , A23K20/163 , A23L29/231 , A23L33/21 , A61P29/00
摘要: This invention relates generally to compositions and methods for combating inflammatory disease and particularly to the use of food and/or feed compositions for preventing, reducing and/or treating inflammatory disorders, diseases, or discomforts, wherein the compositions comprising at least one specific pectin.
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公开(公告)号:US09334504B2
公开(公告)日:2016-05-10
申请号:US14192236
申请日:2014-02-27
申请人: DSM IP ASSETS B.V.
摘要: The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.
摘要翻译: 本发明提供丝状真菌细胞的转染方法,包括提供多个容器,向每个容器填充一定量的转染所需的聚合物,将待转染的细胞以及转染试剂的水溶液加入 每个容器,孵育所得混合物,从培养的混合物中除去转染试剂; 并选择已经转化的细胞,其特征在于培养混合物的总体积小于每个容器1ml。 此外,本发明提供了转化的丝状真菌细胞用于生产蛋白质或代谢物的用途。
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公开(公告)号:US11297860B2
公开(公告)日:2022-04-12
申请号:US15761281
申请日:2016-09-15
申请人: DSM IP Assets B.V.
IPC分类号: A23L27/21 , A23L9/10 , A23C9/156 , A23L2/56 , A23L9/20 , A23L33/14 , A23L23/00 , A23L27/23 , A23L2/60 , A23L23/10 , A23L27/00 , A23L31/15 , C12N9/24
摘要: The present invention relates to a method for producing a flavour composition comprising providing a slurry of yeast cell walls and contacting the slurry of yeast cell walls with a glucanase and with an endoprotease, followed by separating a liquid fraction by solid/liquid separation to provide the liquid flavour composition.
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公开(公告)号:US11098332B2
公开(公告)日:2021-08-24
申请号:US16471589
申请日:2017-12-14
申请人: DSM IP Assets B.V.
摘要: The present invention relates to a method for the enzymatic reduction of cystine to cysteine comprising contacting cystine with a reduction solution comprising: (i) an active glutathione reductase (EC1.8.1.7); (ii) a cofactor; and (iii) glutathione; and recovering a cysteine comprising composition, wherein the reduction solution has a pH of at least 6 during contacting with cystine.
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