利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

11 条记录 (耗时 0.017 秒)

    Method for increasing glycosylation of a composition comprising steviol glycosides
    2.
    发明授权
    Method for increasing glycosylation of a composition comprising steviol glycosides 有权

    公开(公告)号：US10472661B2

    公开(公告)日：2019-11-12

    申请号：US15516854

    申请日：2015-10-08

    申请人： DSM IP ASSETS B.V.

    发明人： Marco Alexander Van Den Berg ,  Marcella Katharina Madern ,  Remko Tsjibbe Winter

    IPC分类号： C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12P19/18

    摘要： The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises: a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.

    HIGH THROUGHPUT TRANSFECTION OF FILAMENTOUS FUNGI
    4.
    发明申请
    HIGH THROUGHPUT TRANSFECTION OF FILAMENTOUS FUNGI 有权
    真菌真菌的高通量转移

    公开(公告)号：US20140178932A1

    公开(公告)日：2014-06-26

    申请号：US14192236

    申请日：2014-02-27

    申请人： DSM IP ASSETS B.V.

    发明人： Marco Alexander Van Den Berg ,  Bianca Elisabeth Maria Gielesen ,  Adriana Marina Riemens

    IPC分类号： C12N15/80

    CPC分类号： C12N15/80 ,  C12P21/02

    摘要： The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.

    摘要翻译： 本发明提供丝状真菌细胞的转染方法，包括提供多个容器，向每个容器填充一定量的转染所需的聚合物，将待转染的细胞以及转染试剂的水溶液加入 每个容器，孵育所得混合物，从培养的混合物中除去转染试剂; 并选择已经转化的细胞，其特征在于培养混合物的总体积小于每个容器1ml。 此外，本发明提供了转化的丝状真菌细胞用于生产蛋白质或代谢物的用途。

    High throughput transfection of filamentous fungi
    8.
    发明授权
    High throughput transfection of filamentous fungi 有权
    高通量转染丝状真菌

    公开(公告)号：US09334504B2

    公开(公告)日：2016-05-10

    申请号：US14192236

    申请日：2014-02-27

    申请人： DSM IP ASSETS B.V.

    发明人： Marco Alexander Van Den Berg ,  Bianca Elisabeth Maria Gielesen ,  Adriana Marina Riemens

    IPC分类号： C12N15/80 ,  C12P21/02

    CPC分类号： C12N15/80 ,  C12P21/02

    摘要： The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.

    摘要翻译： 本发明提供丝状真菌细胞的转染方法，包括提供多个容器，向每个容器填充一定量的转染所需的聚合物，将待转染的细胞以及转染试剂的水溶液加入 每个容器，孵育所得混合物，从培养的混合物中除去转染试剂; 并选择已经转化的细胞，其特征在于培养混合物的总体积小于每个容器1ml。 此外，本发明提供了转化的丝状真菌细胞用于生产蛋白质或代谢物的用途。