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1.发明申请公开(公告)号：US20160129442A1
公开(公告)日：2016-05-12
申请号：US14875418
申请日：2015-10-05
Applicant: Fluidigm Corporation
Inventor： Tim Woudenberg , Jing Wang , Hou-Pu Chou
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01F13/0059 , B01F13/0094 , B01F13/1013 , B01F13/1022 , B01J2219/00317 , B01J2219/00479 , B01J2219/00585 , B01L3/5025 , B01L3/502723 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/027 , B01L2200/0605 , B01L2300/0829 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , B01L2400/082 , B33Y80/00 , G01N1/28 , H04W4/70 , H04W76/30
Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.
Abstract translation: 描述了包括刚性基层和基层上的弹性体层的微流体装置。 弹性体层可以包括用于输送液体试剂的流体通道的至少一部分，以及排气通道，其接纳从流动通道穿过弹性体层扩散的气体并将其从弹性体层排出。 装置还可以包括流体连接到流体通道的混合室，以及与限定流动通道的一部分的可偏转膜重叠的控制通道，其中控制通道可操作以改变液体试剂流动的速率 通过流体通道。 所述装置还可包括弹性体层上的刚性塑料层。
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2.发明申请公开(公告)号：US20130296196A1
公开(公告)日：2013-11-07
申请号：US13781318
申请日：2013-02-28
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A.A. West
IPC: C12Q1/68
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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3.发明申请公开(公告)号：US20130295602A1
公开(公告)日：2013-11-07
申请号：US13781307
申请日：2013-02-28
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A.A. West
IPC: C12Q1/68
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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公开(公告)号：US10227624B2
公开(公告)日：2019-03-12
申请号：US14800548
申请日：2015-07-15
Applicant: Fluidigm Corporation
Inventor： Peilin Chen , Jing Wang , Andrew May
IPC: C12Q1/68 , C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q1/683
Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.
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5.发明授权公开(公告)号：US09506845B2
公开(公告)日：2016-11-29
申请号：US13781318
申请日：2013-02-28
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A. A. West
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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Patent Agency Ranking
公开(公告)号：US09381512B2
公开(公告)日：2016-07-05
申请号：US14071598
申请日：2013-11-04
Applicant: Fluidigm Corporation
Inventor： David S. Cohen , Jing Wang , Andrew May , Robert C. Jones , Hany Nassef
CPC classification number: B01L3/502738 , B01L3/5025 , B01L7/52 , B01L2200/0684 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0487 , B01L2400/0605 , B01L2400/0638 , B01L2400/0655 , B33Y80/00 , F16K99/0001 , F16K99/0026 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , Y10T137/0318 , Y10T137/85938
Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.
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7.发明申请公开(公告)号：US20160017395A1
公开(公告)日：2016-01-21
申请号：US14800548
申请日：2015-07-15
Applicant: Fluidigm Corporation
Inventor： Peilin Chen , Jing Wang , Andrew May
IPC: C12P19/34
CPC classification number: C12P19/34 , C12Q1/6846 , C12Q1/6848 , C12Q2525/113 , C12Q2525/179 , C12Q2527/101 , C12Q2527/125 , C12Q2531/119
Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.
Abstract translation: 本发明提供使用Phi 29 DNA聚合酶的单细胞全基因组扩增的试剂，试剂盒和方法。
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8.发明申请公开(公告)号：US20140308178A1
公开(公告)日：2014-10-16
申请号：US14091344
申请日：2013-11-27
Applicant: Fluidigm Corporation
Inventor： Tim Woudenberg , Jing Wang , Hou-Pu Chou
IPC: G01N1/28
CPC classification number: B01L3/502715 , B01F13/0059 , B01F13/0094 , B01F13/1013 , B01F13/1022 , B01J2219/00317 , B01J2219/00479 , B01J2219/00585 , B01L3/5025 , B01L3/502723 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/027 , B01L2200/0605 , B01L2300/0829 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , B01L2400/082 , B33Y80/00 , G01N1/28 , H04W4/70 , H04W76/30
Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.
Abstract translation: 描述了包括刚性基层和基层上的弹性体层的微流体装置。 弹性体层可以包括用于输送液体试剂的流体通道的至少一部分，以及排气通道，其接纳从流动通道穿过弹性体层扩散的气体并将其从弹性体层排出。 装置还可以包括流体连接到流体通道的混合室，以及与限定流动通道的一部分的可偏转膜重叠的控制通道，其中控制通道可操作以改变液体试剂流动的速率 通过流体通道。 所述装置还可包括弹性体层上的刚性塑料层。
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9.发明申请METHODS, SYSTEMS AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 有权使用微流控法进行单细胞捕获和处理的方法，系统和设备公开(公告)号：US20130302883A1
公开(公告)日：2013-11-14
申请号：US13781292
申请日：2013-02-28
Applicant: FLUIDIGM CORPORATION
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A.A. West
IPC: G01N1/34
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
Abstract translation: 描述了利用微流体的多单细胞捕获和处理的方法，系统和装置。 提供的工具和技术用于捕获，分配和/或操纵来自较大群体的细胞的单个细胞以及产生与每个单个细胞相关的遗传信息和/或反应。 可以使用不同的捕获配置来捕获单个细胞，然后在多室反应配置中处理每个单独的细胞。 一些实施方案可以提供已经从较大群体中划分的多个单个细胞的特异性靶扩增，全基因组扩增，全转录组扩增，实时PCR制备，拷贝数变异，预扩增，mRNA测序和/或单倍型 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置为用于对作为处理的一部分的各个单元或相关联的反应产物进行成像。 在某些情况下可以收获和/或进一步分析反应产物。
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10.发明授权Methods, systems and devices for multiple single-cell capturing and processing using microfluidics 有权使用微流体法进行多单细胞捕获和处理的方法，系统和装置公开(公告)号：US09304065B2
公开(公告)日：2016-04-05
申请号：US13781307
申请日：2013-02-28
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A. A. West
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
Abstract translation: 描述了利用微流体的多单细胞捕获和处理的方法，系统和装置。 提供的工具和技术用于捕获，分配和/或操纵来自较大群体的细胞的单个细胞以及产生与每个单个细胞相关的遗传信息和/或反应。 可以使用不同的捕获配置来捕获单个细胞，然后在多室反应配置中处理每个单独的细胞。 一些实施方案可以提供已经从较大群体中划分的多个单个细胞的特异性靶扩增，全基因组扩增，全转录组扩增，实时PCR制备，拷贝数变异，预扩增，mRNA测序和/或单倍型 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置为用于对作为处理的一部分的各个单元或相关联的反应产物进行成像。 在某些情况下可以收获和/或进一步分析反应产物。
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