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公开(公告)号：US09778263B2
公开(公告)日：2017-10-03
申请号：US14079347
申请日：2013-11-13
Applicant: General Electric Company
Inventor： Srabani Bhaumik , Michael John Gerdes , Zhengyu Pang , Fiona Ginty , Christopher James Sevinsky , Qing Li , Alberto Santamaria-Pang , Yunxia Sui , Keyur Hemantkumar Desai
IPC: G01N33/574 , G01N33/569 , G01N33/543 , G06T7/00 , G06K9/00 , G01N1/30
CPC classification number: G01N33/57492 , G01N1/30 , G01N33/54393 , G01N33/56972 , G01N33/56977 , G06K9/00147 , G06T7/0012 , G06T2207/10024 , G06T2207/30024
Abstract: The present disclosure relates to characterization of biological samples. By way of example, a biological sample may be contacted with a plurality of probes specific for targets in the sample, such as probes for immune markers and segmenting probes. Acquired image data of the sample may be used to segment the images into epithelial and stromal regions to characterize individual cells in the sample based on the binding of the probes. Further, the biological sample may be characterized by a distribution, location, and type of a plurality of the characterized cells.
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公开(公告)号：US20150133321A1
公开(公告)日：2015-05-14
申请号：US14079347
申请日：2013-11-13
Applicant: General Electric Company
Inventor： Srabani Bhaumik , Michael John Gerdes , Zhengyu Pang , Fiona Ginty , Christopher James Sevinsky, JR. , Qing Li , Alberto Santamaria-Pang , Yunxia Sui , Keyur Hemantkumar Desai
IPC: G01N33/574
CPC classification number: G01N33/57492 , G01N1/30 , G01N33/54393 , G01N33/56972 , G01N33/56977 , G06K9/00147 , G06T7/0012 , G06T2207/10024 , G06T2207/30024
Abstract: The present disclosure relates to characterization of biological samples. By way of example, a biological sample may be contacted with a plurality of probes specific for targets in the sample, such as probes for immune markers and segmenting probes. Acquired image data of the sample may be used to segment the images into epithelial and stromal regions to characterize individual cells in the sample based on the binding of the probes. Further, the biological sample may be characterized by a distribution, location, and type of a plurality of the characterized cells.
Abstract translation: 本公开涉及生物样品的表征。 举例来说，可以将生物样品与样品中靶标特异性的多种探针接触，例如用于免疫标记和分割探针的探针。 可以使用样本的获取图像数据将图像分割成上皮和基质区域，以基于探针的结合来表征样品中的各个细胞。 此外，生物样品可以由多个表征的细胞的分布，位置和类型来表征。
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公开(公告)号：US20160357905A1
公开(公告)日：2016-12-08
申请号：US14730032
申请日：2015-06-03
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Brion Daryl Sarachan , John Frederick Graf , Michael John Gerdes
Abstract: Identification of regions-of-interest within cell maps is disclosed. In certain embodiments, identification of the regions-of interest is based on the use of biomarkers selected based on nucleic acid sequence data. The nucleic acid sequence data may be acquired for a homogeneous or heterogeneous set of cells present in the respective tissue sample.
Abstract translation: 公开了细胞图中感兴趣区域的识别。 在某些实施方案中，感兴趣区域的鉴定基于使用基于核酸序列数据选择的生物标志物。 核酸序列数据可以针对存在于各组织样品中的均质或异质的细胞组获得。
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公开(公告)号：US20160289735A1
公开(公告)日：2016-10-06
申请号：US14672358
申请日：2015-03-30
Applicant: General Electric Company
Inventor： Michael John Gerdes , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk
CPC classification number: C12Q1/6804 , C12N15/1003 , C12Q2522/101 , G01N33/689
Abstract: A method is provided herein, wherein the method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample, comprises contacting a lysis solution to the sample comprising at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate comprises a protamine-DNA complex. The method further comprises applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The antibody binding is followed by capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.
Abstract translation: 本文提供了一种方法，其中在样品中捕获精子脱氧核糖核酸（DNA）的方法包括将裂解液与包含至少精子细胞或精细胞裂解物的样品接触以裂解精子细胞。 精子细胞或精子细胞裂解物包含鱼精蛋白-DNA复合物。 该方法还包括向裂解的精子细胞施加至少一种鱼精蛋白特异性抗体，其中鱼精蛋白特异性抗体结合裂解的精细胞的鱼精蛋白-DNA复合物以形成抗体 - 鱼精蛋白-DNA复合物。 抗体结合后，捕获抗体 - 鱼精蛋白-DNA复合物; 并从捕获的抗体 - 鱼精蛋白-DNA复合物中分离和检测精子DNA。
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公开(公告)号：US20140045251A1
公开(公告)日：2014-02-13
申请号：US14060650
申请日：2013-10-23
Applicant: General Electric Company
Inventor： Anup Sood , Michael Christopher Montalto , Michael John Gerdes , Maximilian Lewis Seel , Robert John Filkins
IPC: G01N33/53
CPC classification number: G01N33/53 , C12Q1/68 , G01N33/542 , G01N33/582 , Y10T436/143333
Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.
Abstract translation: 提供了用于检测生物样品中多个靶的方法。 所述方法包括使样品与第一探针接触; 将第一探针物理地绑定到第一靶; 观察来自第一探针的第一信号; 施用化学试剂来修饰第一信号; 使样品与第二探针接触; 将第二探针物理地绑定到第二靶上; 并观察来自第二探测器的第二信号。 本文公开的方法还提供绑定，观察，信号修改的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件和设备。
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Patent Agency Ranking
公开(公告)号：US10030241B2
公开(公告)日：2018-07-24
申请号：US15150806
申请日：2016-05-10
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Patrick McCoy Spooner , Peter James Tatnell , Jeffrey Kenneth Horton , John Richard Nelson , Michael John Gerdes , Suzana Kiel , Ralf Lenigk , Alexander Schenk , Wei Sun
IPC: C12N15/10 , G01N33/53 , C12Q1/6804 , G01N27/447 , G01N33/68 , C12Q1/6888
Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.
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公开(公告)号：US09957548B2
公开(公告)日：2018-05-01
申请号：US14672358
申请日：2015-03-30
Applicant: General Electric Company
Inventor： Michael John Gerdes , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk
CPC classification number: C12Q1/6804 , C12N15/1003 , C12Q2522/101 , G01N33/689
Abstract: A method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample is disclosed. The method includes a step of contacting a lysis solution to the sample that includes at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate includes a protamine-DNA complex. The method further includes applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The method further includes capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.
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公开(公告)号：US20160289666A1
公开(公告)日：2016-10-06
申请号：US15150806
申请日：2016-05-10
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Patrick McCoy Spooner , Peter James Tatnell , Jeffrey Kenneth Horton , John Richard Nelson , Michael John Gerdes , Suzana Kiel , Ralf Lenigk , Alexander Schenk , Wei Sun
IPC: C12N15/10 , C12Q1/68 , G01N27/447 , G01N33/53
CPC classification number: C12N15/1013 , C12N15/101 , C12Q1/6804 , C12Q1/6888 , C12Q2600/124 , G01N27/44743 , G01N27/44747 , G01N33/5308 , G01N33/689 , C12Q2522/101
Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.
Abstract translation: 本发明一般涉及从生物样品中或在生物样品中捕获精子核酸的方法和试剂盒。 在一个实施方案中，该方法包括使样品与裂解溶液接触，其具有鱼精蛋白-DNA复合物，以裂解细胞并施用鱼精蛋白特异性抗体。 这导致鱼精蛋白特异性抗体结合鱼精蛋白-DNA以形成可被捕获，纯化或检测的复合物。 还提供了用于实施所公开的方法的套件。
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公开(公告)号：US09322051B2
公开(公告)日：2016-04-26
申请号：US14047233
申请日：2013-10-07
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Anup Sood , Arunkumar Natarajan , Michael James Rishel , Michael John Gerdes
IPC: C12Q1/68 , G01N33/68 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/543
CPC classification number: C12Q1/6825 , C12Q2523/319 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/6854
Abstract: Disclosed are high throughput methods of probing multiple targets in a biological sample where the recurrent time-consuming antibody incubation steps and individual signal modification and activation steps are replaced by simultaneous hybridization of biomarkers and sequential detection by combining signal removal and activation into a single step. Also disclosed are images obtained by such methods.
Abstract translation: 公开了探测生物样品中多个靶标的高通量方法，其中复发耗时的抗体孵育步骤和单独的信号修饰和激活步骤被生物标志物的同时杂交和通过将信号去除和活化组合到单个步骤中的顺序检测来代替。 还公开了通过这种方法获得的图像。
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公开(公告)号：US10672505B2
公开(公告)日：2020-06-02
申请号：US14730032
申请日：2015-06-03
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Brion Daryl Sarachan , John Frederick Graf , Michael John Gerdes
IPC: G16B45/00 , G16B25/00 , C12Q1/6841
Abstract: Identification of regions-of-interest within cell maps is disclosed. In certain embodiments, identification of the regions-of interest is based on the use of biomarkers selected based on nucleic acid sequence data. The nucleic acid sequence data may be acquired for a homogeneous or heterogeneous set of cells present in the respective tissue sample.
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