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    Methods and compositions for nucleic acid amplification
    1.
    发明授权
    Methods and compositions for nucleic acid amplification 有权

    公开(公告)号：US10415092B2

    公开(公告)日：2019-09-17

    申请号：US15417736

    申请日：2017-01-27

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold ,  Michael M. Becker

    IPC: C12Q1/68 ,  C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/6834 ,  C12Q1/6865

    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

    Asymmetric hairpin target capture oligomers
    2.
    发明授权
    Asymmetric hairpin target capture oligomers 有权

    公开(公告)号：US10655165B2

    公开(公告)日：2020-05-19

    申请号：US14376128

    申请日：2013-02-01

    Applicant: GEN-PROBE INCORPORATED

    Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

    IPC: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6816

    Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

    ASYMMETRIC HAIRPIN TARGET CAPTURE OLIGOMERS
    5.
    发明申请
    ASYMMETRIC HAIRPIN TARGET CAPTURE OLIGOMERS 审中-公开
    不对称的毛发目标捕获低聚物

    公开(公告)号：US20140370506A1

    公开(公告)日：2014-12-18

    申请号：US14376128

    申请日：2013-02-01

    Applicant: GEN-PROBE INCORPORATED

    Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

    IPC: C12Q1/68

    CPC classification number: C12Q1/6834 ,  C12Q1/6816 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2565/501

    Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

    Abstract translation: 本发明提供了改进的茎 - 环目标捕获寡聚体和使用方法。 这种靶捕获低聚物具有靶结合段，其形成两侧为形成茎的茎段的环。 茎段长度不等。 这样的探针在没有靶核酸的情况下显示很少或没有结合固定化的探针，但提供良好的靶灵敏度。 探针在多重检测寡核苷酸多重检测方法中特别有用，用于检测多种靶核酸（例如，检测靶基因的多态多态性）。

    Algorithms for sequence determinations
    7.
    发明授权
    Algorithms for sequence determinations 有权

    公开(公告)号：US11862299B2

    公开(公告)日：2024-01-02

    申请号：US16175742

    申请日：2018-10-30

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Tongjia Yin ,  Steven T. Brentano ,  Jennifer Bungo ,  Xianqun Wang ,  Michael Hadjisavas

    IPC: G16B30/00 ,  G16B30/10

    CPC classification number: G16B30/00 ,  G16B30/10

    Abstract: The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.

    Methods and compositions for nucleic acid amplification
    10.
    发明授权
    Methods and compositions for nucleic acid amplification 有权

    公开(公告)号：US10407723B2

    公开(公告)日：2019-09-10

    申请号：US15595353

    申请日：2017-05-15

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold ,  Michael M. Becker

    IPC: C12Q1/68 ,  C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/6834 ,  C12Q1/6865

    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

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