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    Plant gene construct encoding a protein capable of disrupting the biogenesis of viable pollen
    4.
    发明授权
    Plant gene construct encoding a protein capable of disrupting the biogenesis of viable pollen 失效
    编码能够破坏活花粉生物发生的蛋白质的植物基因构建体

    公开(公告)号：US06172279B2

    公开(公告)日：2001-01-09

    申请号：US08554811

    申请日：1995-11-07

    申请人： Ian George Bridges ,  Simon William Jonathan Bright ,  Andrew James Greenland ,  Wolfgang Walter Schuch

    发明人： Ian George Bridges ,  Simon William Jonathan Bright ,  Andrew James Greenland ,  Wolfgang Walter Schuch

    IPC分类号： C12N1511

    CPC分类号： C12N9/1088 ,  C07K14/005 ,  C07K14/415 ,  C07K14/4703 ,  C07K2319/00 ,  C12N9/88 ,  C12N15/10 ,  C12N15/64 ,  C12N15/73 ,  C12N15/81 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8222 ,  C12N15/8231 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8289 ,  C12N2795/10022 ,  Y10S47/01

    摘要： Male sterility is imparted to a plant by a cascade of gene sequences which expresses a protein which disrupts the biosynthesis of viable pollen. Expression of the disrupter protein is restricted to male parts of the plant by an upstream promoter sequence which is specific to male flowers, the male specific promoter being under control of an operator sequence. The cascade also includes a gene encoding a repressor protein specific for that operator. Expression of the repressor protein is under control of a chemically inducible promoter which is inducible by the application to the plant by, spraying or like process, of an exogenous chemical. In the absence of the exogenous chemical inducer, no repressor protein is expressed, resulting in expression of the disrupter protein and, consequently, male sterility. Fertility may be restored to the plant, when required for maintenance of the line, by spraying with the inducer, resulting in expression of the repressor which binds the operator and inhibits expression of the disrupter protein.

    摘要翻译： 通过表达破坏活花粉生物合成的蛋白质的基因序列的级联赋予植物雄性不育。 破坏蛋白的表达通过对雄性花特异性的上游启动子序列限制在植物的雄性部分，雄性特异性启动子由操作者序列控制。 级联还包括编码针对该操作者特异性的阻遏物蛋白的基因。 抑制蛋白的表达受化学诱导型启动子的控制，其可通过外源化学品的喷雾或类似方法施用于植物而诱导。 在没有外源性化学诱导剂的情况下，没有表达阻抑蛋白，导致破坏蛋白的表达，从而导致雄性不育。 生殖力可以恢复到植物，当需要维持生产线时，通过喷洒诱导剂，导致阻遏物的表达，其结合操作者并抑制破坏蛋白的表达。

    Plants with altered ability to synthesize starch and process for obtaining them
    6.
    发明授权
    Plants with altered ability to synthesize starch and process for obtaining them 失效
    具有合成淀粉能力的植物和获得淀粉的方法

    公开(公告)号：US5792920A

    公开(公告)日：1998-08-11

    申请号：US274366

    申请日：1994-07-13

    申请人： Ian George Bridges ,  Philip Anthony Fentem ,  Wolfgang Walter Schuch ,  Peter Lewis Keeling ,  George William Singletary ,  Mark Olive

    发明人： Ian George Bridges ,  Philip Anthony Fentem ,  Wolfgang Walter Schuch ,  Peter Lewis Keeling ,  George William Singletary ,  Mark Olive

    IPC分类号： C07K16/40 ,  C12N9/12 ,  C12N15/11 ,  C12N15/54 ,  C12N15/82 ,  A01H5/00 ,  C12N15/29

    CPC分类号： C12N15/8245 ,  C07K16/40 ,  C12N9/1241

    摘要： Plants, particularly cereal plants, which have altered ability to synthesise starch are produced by inserting into the genome of the plant a gene encoding an enzyme of the starch biosynthetic pathway such that capacity to produce starch is improved or reduced. The gene encoding the enzyme ADP-glucose pyrophosphorylase has been isolated from wheat leaf and wheat endosperm tissue. The enzyme is essential to the biosynthesis of starch in plants. Addition of extra copies of the gene to a plant by transformation enhances starch production, increasing the food value of the crop. Conversely, insertion of a gene encoding mRNA antisense to the mRNA encoded by the endogenous ADP-glucose pyrophosphorylase gene will reduce starch production. cDNA sequences are provided which may be used to identify homologous sequences in other tissue and in other plants. The ADP-glucose pyrophosphorylase gene promoter may be used in the genetic manipulation of plants to alter their starch-synthesising ability.

    摘要翻译： 已经改变了合成淀粉能力的植物，特别是谷类植物，通过将植物基因组插入到编码淀粉生物合成途径的酶的基因中，从而提高或降低淀粉生产能力。 从小麦叶和小麦胚乳组织中分离出编码酶ADP-葡萄糖焦磷酸化酶的基因。 该酶对植物中淀粉的生物合成至关重要。 通过转化将基因的额外拷贝添加到植物中增强了淀粉的产生，增加了作物的食物价值。 相反，插入编码与内源性ADP-葡萄糖焦磷酸化酶基因编码的mRNA相反的mRNA的基因将降低淀粉产生。 提供了可用于鉴定其他组织和其它植物中的同源序列的cDNA序列。 ADP-葡萄糖焦磷酸化酶基因启动子可用于植物的遗传操作以改变其淀粉合成能力。