公开(公告)号：US20230357307A1

公开(公告)日：2023-11-09

申请号：US18311876

申请日：2023-05-03

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE LTD.

Inventor： Abdul Sadeer Abd Salam ,  Xiangyuan Yang ,  Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Min Yen Lee ,  Ramesh Neelakandan ,  Jeffrey G. Mandell ,  Erin Garcia ,  Sharyuen Soh ,  Daniel Hartoyo Lukamto

IPC: C07H21/00 ,  C12Q1/6869 ,  C07H19/10

CPC classification number: C07H21/00 ,  C12Q1/6869 ,  C07H19/10

Abstract: In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a linker construct between two positions of the nucleotide, wherein the linker construct optionally comprises a reporter moiety corresponding to the identity of the nucleotide, and wherein the linker construct is a part of the cleavable cyclic loop nucleotide comprising a cleavable site. In some embodiments, the nucleotides further comprise arresting constructs for slowing or halting the polynucleotide translocation through a nanopore.

公开(公告)号：US20250101505A1

公开(公告)日：2025-03-27

申请号：US18894517

申请日：2024-09-24

Applicant: Illumina, Inc.

Inventor： Nam Trong Nguyen ,  Xavier von Hatten ,  Xiangyuan Yang ,  Eric Brustad ,  Will Tovey ,  Matthew Worsdale ,  Gianluca Artioli ,  Teodora Gal

IPC: C12Q1/6853

Abstract: In some examples, a dendritic molecule may include a dendritic core; a seeding primer coupled to the dendritic core; and a plurality of dendrons, each of the dendrons comprising an inert, elongated polymer comprising a first end coupled to the dendritic core and a second end coupled to a first functional group, wherein the first functional group is to react with a second functional group to form a covalent bond. In other examples, a dendritic molecule may include a dendritic core; a single-stranded polynucleotide covalently bonded to the dendritic core; and a plurality of dendrons, each of the dendrons comprising an inert, elongated polymer comprising a first end coupled to the dendritic core and a second end.

公开(公告)号：US20230383342A1

公开(公告)日：2023-11-30

申请号：US18325610

申请日：2023-05-30

Applicant: Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd. ,  Illumina, Inc. ,  Illumina Software, Inc.

Inventor： Xiaolin Wu ,  Patrick McCauley ,  Madushani Dharmarwardana ,  Saurabh Nirantar ,  Misha Golynskiy ,  Xiangyuan Yang ,  Ramesh Neelakandan ,  Benedict Mackworth ,  Carole Anastasi ,  Aathavan Karunakaran ,  Gery M. Vessere ,  David Bracher

IPC: C12Q1/6869 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  G01N2021/6439 ,  G01N2021/6419 ,  G01N21/6428

Abstract: Embodiments of the present disclosure relate to kits, compositions, and methods for nucleic acid sequencing, for example, two-channel nucleic acid sequencing by synthesis using blue and green light excitation. In particular, unlabeled nucleotides for incorporation may be used in conjunction with affinity reagents containing detectable labels excitable by blue and/or green lights, for specific binding to each type of nucleotides incorporated.

公开(公告)号：US20250109433A1

公开(公告)日：2025-04-03

申请号：US18887792

申请日：2024-09-17

Applicant: Illumina, Inc.

Inventor： Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Ramesh Neelakandan ,  Erin Garcia ,  Abdul Sadeer Abd Salam ,  Daniel Hartoyo Lukamto ,  Soumadwip Ghosh ,  Xiangyuan Yang ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6853

Abstract: In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a macromolecular block. In some embodiments, the macromolecular blocks are configured for slowing or halting the polynucleotide translocation through a nanopore. In some embodiments the macromolecular blocks have linear and branched structures.

公开(公告)号：US20240240217A1

公开(公告)日：2024-07-18

申请号：US18530865

申请日：2023-12-06

Applicant: Illumina, Inc.

Inventor： Jean-Alexandre Richard ,  Xiangyuan Yang ,  Daniel Hartoyo Lukamto ,  Ramesh Neelakandan

IPC: C12P19/34 ,  C07H15/26 ,  C07H19/048 ,  C12Q1/6869 ,  G01N21/64

CPC classification number: C12P19/34 ,  C07H15/26 ,  C07H19/048 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N2021/6439

Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with 3′ vinyl or isonitrile containing blocking groups and/or tetrazine or strained unsaturated ring containing cleavable linkers. Additionally, the present disclosure provides methods of using the nucleoside/nucleotide in oligonucleotide synthesis, and methods of sequencing using the nucleotide described herein.

公开(公告)号：US20250109434A1

公开(公告)日：2025-04-03

申请号：US18900392

申请日：2024-09-27

Applicant: Illumina, Inc.

Inventor： Hugo Brandão ,  John Moon ,  Jeffrey G. Mandell ,  Erin Garcia ,  Lisa Savagian ,  Eunho Noh ,  Xiangyuan Yang ,  Sharyuen Soh ,  Sadeer Salam

IPC: C12Q1/6869 ,  C12Q1/6809

Abstract: A polymer is disposed through a nanopore that encodes a polynucleotide's sequence and includes monomer units that encode nucleotides and include first and second reporter moieties and first and second arresting constructs. The first reporter moiety is translocated into the nanopore's aperture, where a first value of an electrical property of the first reporter moiety is measured while the first arresting construct pauses translocation. The second reporter moiety is translocated into the aperture, where a second value of an electrical property of the second reporter moiety is measured while the second arresting construct pauses translocation. The first value and the second value for each monomer unit is used to identify the nucleotide encoded by that monomer unit; and distinguish the nucleotide encoded by that monomer unit from the nucleotides encoded by adjacent monomer units, including by those that encode the same type of nucleotide as that monomer unit.

公开(公告)号：US10851131B2

公开(公告)日：2020-12-01

申请号：US16627035

申请日：2019-02-19

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Jeffrey Mandell ,  Silvia Gravina ,  Sergio Peisajovich ,  Kaitlin Pugliese ,  Yin Nah Teo ,  Xiangyuan Yang ,  Maria Candelaria Rogert Bacigalupo

IPC: C07H21/00 ,  C12Q1/6869 ,  C40B70/00

Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I:

公开(公告)号：US20250019758A1

公开(公告)日：2025-01-16

申请号：US18650800

申请日：2024-04-30

Applicant: ILLUMINA, INC.

Inventor： Abdul Sadeer Abd Salam ,  Xiangyuan Yang ,  Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Tsz Ying Yuen ,  Daniel Hartoyo Lukamto ,  Erin Garcia ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  C12Q1/6806

Abstract: Provided herein are methods for modification-based controlled polynucleotide translocation in nanopores for sequencing, modified nucleotides, and kits and systems for performing the disclosed methods. In some embodiments, modifications can be used to control polynucleotide translocation by modifying nucleotides on a strand of polynucleotide to carry a modification, where the modifications can arrest or slow translocation when encountering the nanopore. In some embodiments, application of a voltage can move one nucleotide and its attached modification through the nanopore at a time.

公开(公告)号：US20240317793A1

公开(公告)日：2024-09-26

申请号：US18608556

申请日：2024-03-18

Applicant: Illumina, Inc.

Inventor： Hassan Bohra ,  Xiangyuan Yang ,  Ramesh Neelakandan ,  Yin Nah Teo ,  Abdul Sadeer Abd Salam ,  Eric Murtfeldt

IPC: C07H21/00

CPC classification number: C07H21/00

Abstract: In some examples, a nucleoside triphosphate analogue may include a sugar, a nucleobase coupled to the sugar, a triphosphate group coupled to the sugar, a heteroatom coupled to an alpha phosphate of the triphosphate group, and a first substituent coupled to the heteroatom. The heteroatom may be selected from the group consisting of oxygen, nitrogen, and carbon. The first substituent may include at least one of an alkyl chain or a polymer.

公开(公告)号：US11578094B2

公开(公告)日：2023-02-14

申请号：US17106963

申请日：2020-11-30

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Jeffrey Mandell ,  Silvia Gravina ,  Sergio Peisajovich ,  Kaitlin Pugliese ,  Yin Nah Teo ,  Xiangyuan Yang ,  Maria Candelaria Rogert Bacigalupo

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6869 ,  C40B70/00

Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I: