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    GROUP OF NOVEL ENANTIOSLEECTIVE MICROBIAL NITRILE HYDRATASES WITH BROAD SUBSTRATE SPECIFICITY
    1.
    发明申请
    GROUP OF NOVEL ENANTIOSLEECTIVE MICROBIAL NITRILE HYDRATASES WITH BROAD SUBSTRATE SPECIFICITY 失效
    具有广泛基质特征的新型强化微生物氮化物组

    公开(公告)号：US20100047863A1

    公开(公告)日：2010-02-25

    申请号：US12296057

    申请日：2007-04-05

    申请人： Jürgen Eck ,  Klaus Liebeton ,  Lutz Fischer ,  Sabine Lutz-Wahl ,  Martina Hensel ,  Christian Ewert ,  Marcus Wälz

    发明人： Jürgen Eck ,  Klaus Liebeton ,  Lutz Fischer ,  Sabine Lutz-Wahl ,  Martina Hensel ,  Christian Ewert ,  Marcus Wälz

    IPC分类号： C12P21/06 ,  C12P13/02 ,  C12N15/74 ,  C12N1/21 ,  C12N15/63 ,  C07K14/00 ,  C07K16/00 ,  C07H21/04

    CPC分类号： C12N9/88 ,  C12P13/02 ,  C12P41/006

    摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitrites.

    摘要翻译： 本发明提供了编码具有腈水合酶（NHase）[E.C.]的酶的多核苷酸或一对多核苷酸。 4.2.1.84]活动。 此外，提供了包含所公开的多核苷酸或一对多核苷酸的载体和宿主及其生产方法。 此外，本发明涉及一对多肽或具有NHase活性的融合蛋白，特异性结合所述一对多肽或融合蛋白的抗体，在严格条件下与所公开的多核苷酸特异性杂交的引物或探针，或者 一对多核苷酸，包含多核苷酸或一对多核苷酸的组合物，一对多肽或融合蛋白，本发明的抗体和/或一种或多种引物或探针以及生产酰胺的方法，其包括对映选择性转化 亚硝酸盐

    Group of novel enantioselective microbial nitrile hydratases with broad substrate specificity
    2.
    发明授权
    Group of novel enantioselective microbial nitrile hydratases with broad substrate specificity 失效
    一组具有广泛底物特异性的新型对映选择性微生物腈水合酶

    公开(公告)号：US08470576B2

    公开(公告)日：2013-06-25

    申请号：US12296057

    申请日：2007-04-05

    申请人： Jürgen Eck ,  Klaus Liebeton ,  Lutz Fischer ,  Sabine Lutz-Wahl ,  Martina Hensel ,  Christian Ewert ,  Marcus Wälz

    发明人： Jürgen Eck ,  Klaus Liebeton ,  Lutz Fischer ,  Sabine Lutz-Wahl ,  Martina Hensel ,  Christian Ewert ,  Marcus Wälz

    IPC分类号： C12N9/78 ,  C12N15/00 ,  C12N1/20 ,  C12P21/06 ,  C12P21/04 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N9/88 ,  C12P13/02 ,  C12P41/006

    摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.

    摘要翻译： 本发明提供了编码具有腈水合酶（NHase）[E.C.]的酶的多核苷酸或一对多核苷酸。 4.2.1.84]活动。 此外，提供了包含所公开的多核苷酸或一对多核苷酸的载体和宿主及其生产方法。 此外，本发明涉及一对多肽或具有NHase活性的融合蛋白，特异性结合所述一对多肽或融合蛋白的抗体，在严格条件下与所公开的多核苷酸特异性杂交的引物或探针，或者 一对多核苷酸，包含多核苷酸或一对多核苷酸的组合物，一对多肽或融合蛋白，本发明的抗体和/或一种或多种引物或探针以及生产酰胺的方法，其包括对映选择性转化 腈类

    GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS
    3.
    发明申请
    GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 有权

    公开(公告)号：US20090311745A1

    公开(公告)日：2009-12-17

    申请号：US12300275

    申请日：2007-05-03

    申请人： Klaus Liebeton ,  Jürgen Eck ,  Uwe Bornscheuer ,  Dominique Böttcher ,  Peter Langer ,  Esen Bellur

    发明人： Klaus Liebeton ,  Jürgen Eck ,  Uwe Bornscheuer ,  Dominique Böttcher ,  Peter Langer ,  Esen Bellur

    IPC分类号： C12P21/00 ,  C12P7/62 ,  C12P7/40 ,  C12P7/02 ,  C12N15/00 ,  C12N15/74 ,  C12N9/18 ,  C12N1/21 ,  C12N15/63 ,  C07K16/40 ,  C07H21/00

    CPC分类号： C12N9/18

    摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (f) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a process for producing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, comprising culturing the host of the present invention and recovering the polypeptide produced by said host. Moreover, The present invention also relates to a process for producing bacteria or eukaryotic cells capable of expressing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, the process comprising genetically engineering bacteria or eukaryotic cells with the vector of the present invention. Finally, the present invention relates to (poly)peptides, antibodies, compositions and various methods for the production of optically active compounds.

    Reca-negative and rhab-negative microorganism
    8.
    发明申请
    Reca-negative and rhab-negative microorganism 审中-公开
    Reca阴性和Rhab阴性微生物

    公开(公告)号：US20070128689A1

    公开(公告)日：2007-06-07

    申请号：US10492939

    申请日：2002-11-11

    申请人： Oliver May ,  Klaus Liebeton ,  Jurgen Eck

    发明人： Oliver May ,  Klaus Liebeton ,  Jurgen Eck

    IPC分类号： C12P21/06 ,  C12N9/16 ,  C12N15/74 ,  C07H21/04 ,  C12N1/21

    CPC分类号： C12R1/19 ,  C12N15/68 ,  C12N15/70 ,  C12P13/04 ,  C12P21/02 ,  C12P41/006

    摘要： The present invention describes new microorganisms specifically for use as expression systems for rhamnose-in-ducible promoters. Due to the combination of rhamnose and recombination deficiency artificially created in these organisms, ex-pression systems which require less of the expensive rhamnose for induction and therefore can be induced more inexpensively, and have a higher genetic stability are obtained.

    摘要翻译： 本发明描述了专门用作用于鼠李糖苷可启动子的表达系统的新微生物。 由于在这些生物体中人造产生的鼠李糖和重组缺陷的组合，需要较少昂贵的鼠李糖用于诱导并因此可以更廉价地诱导并且具有较高遗传稳定性的预压系统。