-
1.发明授权公开(公告)号：US09273345B2
公开(公告)日：2016-03-01
申请号：US11965039
申请日：2007-12-27
IPC分类号： C12N15/115 , C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/115 , Y10T436/143333 , C12Q2525/205 , C12Q2565/101
摘要： Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
摘要翻译： 本文的实施方案涉及用于制备和使用适体的组合物和方法，例如DNA适体（DCE）和/或RNA适体。 在一些实施方案中，方法涉及制备和扩增目标DCE。 在某些实施方案中，用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。 在一些实施方案中，本文公开的方法可用于快速产生大量适配体，例如针对特定目标试剂的DCE。 一些实施例涉及用于执行适配体的自动生成的系统。
-
2.发明申请METHODS AND COMPOSITIONS FOR PROCESSES OF RAPID SELECTION AND PRODUCTION OF NUCLEIC ACID APTAMERS 有权快速选择和生产核酸APTAMERS的方法和组合物公开(公告)号：US20090004644A1
公开(公告)日：2009-01-01
申请号：US11965039
申请日：2007-12-27
IPC分类号： C12Q1/70 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6811 , C12N15/115 , Y10T436/143333 , C12Q2525/205 , C12Q2565/101
摘要： Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
摘要翻译： 本文的实施方案涉及用于制备和使用适体的组合物和方法，例如DNA适体（DCE）和/或RNA适体。 在一些实施方案中，方法涉及制备和扩增目标DCE。 在某些实施方案中，用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。 在一些实施方案中，本文公开的方法可用于快速产生大量适配体，例如针对特定目标试剂的DCE。 一些实施例涉及用于执行适配体的自动生成的系统。
-
公开(公告)号：US5658673A
公开(公告)日：1997-08-19
申请号：US504306
申请日：1995-07-20
CPC分类号： C03C17/3405 , G01R29/0857 , C03C2217/42 , C03C2217/475 , G01R29/0878 , Y10T428/31511 , Y10T428/31515 , Y10T428/31522 , Y10T428/31525 , Y10T428/31529 , Y10T428/31551 , Y10T428/31591 , Y10T428/31605 , Y10T428/31609 , Y10T428/31725 , Y10T428/31768 , Y10T428/31772 , Y10T428/31775 , Y10T428/31779 , Y10T428/31783 , Y10T428/31844 , Y10T428/31848
摘要： An article which is sensitive to non-ionizing electromagnetic radiation in the radiofrequency radiation range, consisting essentially of a substrate, a layer of a binder material and a layer of activated diazoluminomelanin. This article is prepared by coating at least one surface of a substrate with a binder, immersing the binder-coated substrate into a solution comprising luminol, 3AT and a soluble nitrite for a period of about 2 to 12 days to provide a layer of diazoluminomelanin (DALM) on the binder layer, removing the DALM-coated film from the solution and rinsing the same, activating the DALM with sodium bicarbonate and hydrogen peroxide for a period of about 2 to 12 days.
摘要翻译： 在射频辐射范围内对非电离电磁辐射敏感的物品，其基本上由基底，粘合剂材料层和活化的二叠氮木兰素层组成。 本文通过用粘合剂涂覆基材的至少一个表面来制备，将粘合剂涂覆的基材浸入包含鲁米诺3AT和可溶性亚硝酸盐的溶液中约2至12天的时间以提供一层二叠氮木兰宁（ DALM），从溶液中除去DALM涂膜并漂洗，用碳酸氢钠和过氧化氢活化DALM约2-12天。
-
公开(公告)号：US08383036B2
公开(公告)日：2013-02-26
申请号：US12973681
申请日：2010-12-20
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
-
5.发明授权公开(公告)号：US07892484B2
公开(公告)日：2011-02-22
申请号：US10291336
申请日：2002-11-08
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
-
6.发明授权
公开(公告)号：US5856108A
公开(公告)日：1999-01-05
申请号：US779694
申请日：1991-10-21
CPC分类号： C12N9/0036 , C12P17/12 , C12Q1/12 , G01N33/582
摘要： There is provided a method for producing diazoluminomelanin (DALM) which comprises culturing in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, a microorganism containing nitrate reductase.Also provided is a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube and activating luminescence.Further, there is provided a method for producing diazomelanin (DM) which comprises culturing in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, a microorganism containing nitrate reductase.Yet further, there is provided a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube, adding luminol and activating luminescence.
摘要翻译： 提供了一种生产重氮酚蓝蛋白（DALM）的方法，该方法包括在合适的代谢条件下，在含硝酸盐，3-氨基-L-酪氨酸（3-AT）和鲁米诺的培养基中培养含有硝酸还原酶的微生物。 还提供了一种用于直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐，3-氨基-L-酪氨酸（3-AT）和 将鲁米诺转移到适当的代谢条件下，将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管并激活发光。 此外，提供了一种生产重氮木兰素（DM）的方法，其包括在适当的代谢条件下在含有硝酸盐和3-氨基-L-酪氨酸（3-AT）的培养基中培养含有硝酸还原酶的微生物。 此外，提供了一种直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐和3-氨基-L-酪氨酸（3- AT），将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管，加入鲁米诺并激活发光。
-
7.发明授权Compositions, methods and uses for biosynthetic plasmid integrated capture elements 有权生物合成质粒综合捕获元素的组成，方法和用途公开(公告)号：US08628955B2
公开(公告)日：2014-01-14
申请号：US12792492
申请日：2010-06-02
申请人： Johnathan L. Kiel , Amanda Tijerina , Eric A. Holwitt , Jill Parker , Mark A. Sloan , Melanie Woitaske , Maomian Fan
发明人： Johnathan L. Kiel , Amanda Tijerina , Eric A. Holwitt , Jill Parker , Mark A. Sloan , Melanie Woitaske , Maomian Fan
CPC分类号： B82Y5/00 , C12N15/111 , C12N2320/32 , C12N2330/00 , C12P19/34
摘要： Embodiments herein report compositions, systems and methods for making and using plasmid vectors and nanotube complexes. In certain embodiments, compositions, systems and methods herein include making plasmid vectors having aptamer inserts. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of plasmid vectors having aptamer inserts directed to a particular target agent. Other aspects concern plasmid constructs associated with organic semiconductors. Yet other aspects concern complexes of nanotubes associated with dsDNA aptamers and tracking molecules.
摘要翻译： 本文的实施方案报道了用于制备和使用质粒载体和纳米管复合物的组合物，系统和方法。 在某些实施方案中，本文的组合物，系统和方法包括制备具有适体插入片段的质粒载体。 在一些实施方案中，本文公开的方法可用于快速产生具有针对特定目标试剂的适配子插入物的大量质粒载体。 其他方面涉及与有机半导体相关的质粒构建体。 其他方面涉及与dsDNA适体和跟踪分子相关的纳米管的复合物。
-
公开(公告)号：US20120134877A1
公开(公告)日：2012-05-31
申请号：US12973681
申请日：2010-12-20
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
-
公开(公告)号：US06303316B1
公开(公告)日：2001-10-16
申请号：US09608706
申请日：2000-06-30
申请人： Johnathan L. Kiel , John G. Bruno , Jill E. Parker , John L. Alls , Charles R. Batishko , Eric A. Holwitt
发明人： Johnathan L. Kiel , John G. Bruno , Jill E. Parker , John L. Alls , Charles R. Batishko , Eric A. Holwitt
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , C12Q1/6837 , Y10T436/143333 , C12Q2565/607
摘要： In a recognition complex system, nucleic acid ligands comprising random DNA sequences are operatively coupled to an organic semiconductor and distributed so as to form an array of recognition complexes. When an unknown chemical or biological analyte is applied to the array, the electrical and/or photochemical properties of one or more of the recognition complexes are altered upon binding of the nucleic acid ligand to the analyte. The degree to which the electrical and/or photochemical properties change is a function of the affinity of the nucleic acid ligand sequence for the analyte. The electrical and photochemical changes associated with the array, as a whole, can be used as a unique signature to identify the analyte. In certain embodiments, an iterative process of selection and amplification of nucleic acid ligands that bind to the analyte can be used to generate a new array with greater affinity and specificity for a target analyte, or to produce one or more nucleic acid ligands with high binding affinity for an analyte. The present invention also provides methods for preparing nucleic acid ligands that bind with high affinity to an analyte and using such nucleic acid ligands to neutralize the analyte.
摘要翻译： 在识别复合体系中，包含随机DNA序列的核酸配体可操作地偶联到有机半导体上，并分布以形成识别复合物的阵列。 当将未知的化学或生物分析物施加到阵列时，一个或多个识别复合物的电和/或光化学性质在核酸配体与分析物结合时被改变。 电和/或光化学性质变化的程度是核酸配体序列对分析物的亲和力的函数。 与阵列相关联的电学和光化学变化作为一个整体，可以用作识别分析物的独特标记。 在某些实施方案中，结合分析物的核酸配体的选择和扩增的迭代过程可用于产生对靶分析物具有更大亲和性和特异性的新阵列，或产生具有高结合力的一个或多个核酸配体 对分析物的亲和力。 本发明还提供了制备以高亲和力结合分析物并使用这种核酸配体中和分析物的核酸配体的方法。
-
公开(公告)号：US5028541A
公开(公告)日：1991-07-02
申请号：US56034
申请日：1987-06-01
IPC分类号： C12M3/00
摘要： A flow-through cell cultivation apparatus and method is described. The flow-through cell cultivation system comprises a flowcell, a chamber inside the flowcell for holding cells, and a flowcell holder. The flowcell has a lower intake port for flowing liquid nutrient through a cellbed inside the flowcell and an upper outlet port for flowing the liquid nutrient out of the flowcell. The flowcell also includes a chuck for receipt of a thermal probe. The probe is made of electromagnetically non-interactive material. The flowcell is enclosed inside the flowcell holder. The flowcell holder includes a pair of intake ports into a cavity having an open end at the flowcell for turbulently flowing a temperature controlled gas against the flowcell. The cavity has rough walls to promote the turbulent flow. The flowcell holder includes an exhaust port for flowing the gas out of the flowcell holder which also serves as a port for another thermal probe. A one-way valve prevents flow of the liquid nutrient out of the chamber through the intake port. Temperature controlled air is supplied to the flowcell holder by dividing air from an air supply into two conduits. One conduit travels through a coil inside a refrigerated liquid bath and the other through a coil inside a heated liquid bath. The heated and cooled air are recombined to make an even temperature air supply which is then divided once more to provide the two air supplies for creating a turbulent supply of temperature controlled air through the two flowcell holder intake ports. The flowcell may include on one side a semi-permeable membrane for introducing a gas/liquid interface to the cellbed.
摘要翻译： 描述了流通细胞培养装置和方法。 流通池培养系统包括流通池，用于保持电池的流通池内的室和流通池保持器。 流通池具有用于使液体营养物流过流动池内的池底的较低进气口和用于使液体营养物流出流动池的上部出口。 流通池还包括用于接收热探针的卡盘。 探头由电磁非交互材料制成。 流通池封闭在流通池支架内。 流通池保持器包括一对进入端口，其具有在流通池处具有开口端的空腔，用于将受温度控制的气体湍流地流向流动池。 空腔具有粗糙的壁，以促进湍流。 流通池保持器包括用于将气体从流动池保持器流出的排气口，其还用作另一热探针的端口。 单向阀防止液体营养物质通过进气口流出室外。 通过将空气从空气供应分成两个管道，将温度控制的空气供应到流通池保持器。 一个导管穿过冷藏液槽内的线圈，另一个通过加热液槽内的线圈。 加热和冷却的空气被重新组合以产生均匀的温度空气供应，然后再分配一次，以提供两个空气供应，以通过两个流通池保持器进气口产生温度受控空气的湍流供应。 流动池可以在一侧包括用于将气体/液体界面引入细胞床的半透膜。
-
-
-
-
-
-
-
-
-
搜索结果
29 条记录 (耗时 0.037 秒)
