公开(公告)号：US20230313241A1

公开(公告)日：2023-10-05

申请号：US18059330

申请日：2022-11-28

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ja Kyong KO ,  Youngyun JO ,  Gyeongtaek GONG ,  Jung Ho AHN ,  Sun Mi LEE ,  Youngsoon UM

IPC: C12P7/625 ,  C12N15/74 ,  C12N9/10

CPC classification number: C12P7/625 ,  C12N15/74 ,  C12N9/1022 ,  C12Y202/01006 ,  C12N9/1029 ,  C12Y203/01009 ,  C12Y203/01182

Abstract: The present disclosure provides a recombinant microorganism for producing PHBV, a method for preparing the same, and a method for producing PHBV using the microorganism. The present disclosure may provide a recombinant microorganism capable of producing PHBV, which is a biodegradable plastic material with superior physical properties, directly from an inexpensive single carbon source with high efficiency without supplementation of organic acid. The present disclosure can enhance the utilization of PHA, which is expensive and has limited physical properties, and can also provide a technology more effective for industrialization using an inexpensive single carbon source. The PHBV produced according to an exemplary embodiment of the present disclosure can be used not only for general-purpose inexpensive products such as ecofriendly packing materials but also as a high-value-added medical biopolymer.

公开(公告)号：US20190161511A1

公开(公告)日：2019-05-30

申请号：US16198017

申请日：2018-11-21

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Youngsoon UM ,  Kyung Min LEE ,  Sun-Mi LEE ,  Hong Gon KIM ,  Gyeongtaek GONG

IPC: C07H1/06 ,  C07H3/02 ,  C12P7/06 ,  C10L1/02 ,  C25B3/00 ,  C25B9/08

CPC classification number: C07H1/06 ,  C07H3/02 ,  C10L1/02 ,  C10L2200/0469 ,  C10L2290/26 ,  C10L2290/38 ,  C10L2290/548 ,  C12P7/06 ,  C25B3/00 ,  C25B9/08

Abstract: The present disclosure relates to a method for separating sugars and acids with reduced energy consumption, including a step of diffusively dialyzing a first acid hydrolysate obtained by saccharifying biomass with an acid solution, thereby preparing a second acid hydrolysate wherein the concentration of the acid solution contained in the acid hydrolysate is decreased; and a step of electrolyzing the second acid hydrolysate, thereby separating sugars from the acid solution, which is advantageous in that less energy is consumed, the separated acid solution can be recycled directly without further treatment due to high concentration and loss of sugars can be minimized.

公开(公告)号：US20160130620A1

公开(公告)日：2016-05-12

申请号：US14934710

申请日：2015-11-06

Applicant: Korea Institute of Science and Technology

Inventor： Yunje KIM ,  Youngsoon UM ,  Han Min WOO ,  Gyeongtaek GONG ,  Kyoungseon MIN

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12N9/0061 ,  C12N9/0065 ,  C12P19/02 ,  Y02E50/343

Abstract: The present disclosure relates to a method for simultaneously degrading lignin and cellulose and for boosting effect on the cellulase activity using a specific catalyst. Since the present disclosure allows for the preparation of sugars by degrading not only lignin but also cellulose and hemicellulose using the enzymes which were previously known only as lignin-degrading biocatalysts, it provides the advantage that the preparation of a hydrolysate as a source material for the production of biofuels or biochemicals from lignocellulosic biomass can be simplified and facilitated. As a result, the present disclosure can reduce enzyme cost and can provide improved production efficiency by simplifying the biofuel production process.

Abstract translation: 本公开内容涉及同时降解木质素和纤维素并且使用特定催化剂对纤维素酶活性的促进作用的方法。 由于本公开允许通过使用先前已知的仅作为木质素降解生物催化剂的酶降解木质素而且降解纤维素和半纤维素来制备糖，因此其具有以下优点：制备水解产物作为 可以简化和促进从木质纤维素生物质生产生物燃料或生物化学品。 结果，本公开可以降低酶成本，并且可以通过简化生物燃料生产过程来提供提高的生产效率。

公开(公告)号：US20160289712A1

公开(公告)日：2016-10-06

申请号：US15088935

申请日：2016-04-01

Applicant: Korea Institute Of Science And Technology

Inventor： Youngsoon UM ,  Han Min WOO ,  Gyeongtaek GONG ,  Eun Sook KIM ,  Sukhyeong CHO

IPC: C12P7/56 ,  C12N1/20 ,  C12N9/42 ,  C12P7/54 ,  C12P7/40 ,  C12P7/10 ,  C12R1/01 ,  C12N1/22

CPC classification number: C12P7/56 ,  C12N1/20 ,  C12N1/22 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  C12P7/40 ,  C12P7/54 ,  C12P19/02 ,  C12P19/12 ,  C12R1/01 ,  C12Y302/01004 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present disclosure relates to a novel strain capable of saccharifying and fermenting biomass-derived cellulose and a recombinant strain thereof with improved biomass saccharification capability. The present disclosure also relates to a method for producing a material useful as a bioenergy source material such as ethanol, acetic acid, formic acid, etc. using the strain or the recombinant strain. The strain or the recombinant strain may be usefully used in bioenergy industry.

Abstract translation: 本公开涉及能够糖化和发酵生物质衍生的纤维素的新菌株及其具有改善的生物质糖化能力的重组菌株。 本公开还涉及使用该菌株或重组菌株生产可用作生物能源材料如乙醇，乙酸，甲酸等的材料的方法。 菌株或重组菌株可用于生物能源工业。

公开(公告)号：US20160168580A1

公开(公告)日：2016-06-16

申请号：US14860373

申请日：2015-09-21

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Jun Won CHWA ,  Youngsoon UM ,  Gyeong Taek GONG ,  Yunje KIM

IPC: C12N15/74

CPC classification number: C12N15/74

Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.

Abstract translation: 本公开公开了可以用于蓝细菌和大肠杆菌的载体，其包含顺序地作为复制起点的pUC复制起点; 壮观霉素抗性基因作为选择标记; 和选自trc启动子，tetA启动子或修饰的tetA启动子，BAD启动子和cbbL启动子的启动子。 可以使用用载体转化的宿主细胞有效地产生工业上有用的物质。 此外，载体可以用于通过简单组合插入各种靶基因，结果可以有效地制备各种载体。

公开(公告)号：US20160152942A1

公开(公告)日：2016-06-02

申请号：US14679622

申请日：2015-04-06

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Youngsoon UM ,  Gyeong Taek GONG ,  Dong Jin SUH ,  Yunje KIM

IPC: C12N1/20

CPC classification number: C12N1/20 ,  C12R1/01

Abstract: A method for isolating a highly furfural-resistant strain is disclosed. The method includes (A) mixing soil with an isotonic solution and collecting the soil supernatant from the mixture, (B) diluting the soil supernatant and spreading the dilution on a medium supplemented with carboxymethyl cellulose as a nutrient source, and (C) growing a desired strain by culture in the medium spread with the diluted soil supernatant and isolating the strain. Also disclosed is a strain isolated by the method. The strain is Enterobacter cloacae strain GGT036 [Accession No. KCTC 12672BP].

Abstract translation: 公开了一种分离高糠醛抗性菌株的方法。 该方法包括（A）将土壤与等渗溶液混合并从混合物中收集土壤上清液，（B）稀释土壤上清液并将稀释液铺展在补充有羧甲基纤维素作为营养源的培养基上，（C） 通过在稀释的土壤上清液中培养的培养物中所需的菌株并分离菌株。 还公开了通过该方法分离的菌株。 菌株为阴沟肠杆菌菌株GGT036 [保藏号KCTC 12672BP]。

公开(公告)号：US20160024535A1

公开(公告)日：2016-01-28

申请号：US14874040

申请日：2015-10-02

Applicant: Korea Institute of Science and Technology

Inventor： Youngsoon UM ,  Yunje KIM ,  Dongjin SUH ,  Kyung Min LEE ,  Jeong Myeong HA

IPC: C12P7/52 ,  C12P7/16

CPC classification number: C12P7/52 ,  C12P7/10 ,  C12P7/16 ,  C12P7/64 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/343

Abstract: Provided is a method for producing a saccharified solution of wood-based biomass having toxicity reduced or removed, comprising the steps of: preparing a saccharified solution in which wood-based biomass has been pretreated by hydrolysis; and reducing toxicity in the saccharified solution using an electrochemical detoxification method. The method is capable of efficiently removing the toxicity of compounds which are produced during the pretreatment process and inhibit growth of microorganisms and fermentation. In addition, the method is capable of increasing the efficiency of production by minimizing sugar loss and additional costs during the toxicity removal process.

Abstract translation: 本发明提供一种生产具有毒性降低或去除的木质生物质的糖化溶液的方法，包括以下步骤：制备通过水解预处理木质生物质的糖化溶液; 并使用电化学解毒方法降低糖化溶液中的毒性。 该方法能够有效地去除在预处理过程中产生的化合物的毒性并抑制微生物的生长和发酵。 此外，该方法能够通过最小化糖损失和毒性去除过程中的额外成本来提高生产效率。

公开(公告)号：US20240117389A1

公开(公告)日：2024-04-11

申请号：US18470284

申请日：2023-09-19

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Youngsoon UM ,  Deurim YUN ,  Sun Mi LEE ,  Gyeongtaek GONG ,  Ja Kyong KO ,  Jung Ho AHN

IPC: C12P7/40 ,  C12P7/02 ,  C12P7/16 ,  C12P7/52

CPC classification number: C12P7/40 ,  C12P7/02 ,  C12P7/16 ,  C12P7/52

Abstract: The present disclosure is to provide a method capable of producing an organic acid in high yield from an acetic acid strain using synthesis gas as a substrate. According to the present disclosure, the productivity of metabolites with C2 to C6 carbon atoms derived from synthesis gas and the selectivity of hexanoic acid production among metabolites can be improved through a first fermentation step of simultaneously providing a substrate comprising synthesis gas and sugar; and a second fermentation step of semi-mixotrophic fermentation of providing only the substrate comprising the synthesis gas. Therefore, the disclosure can contribute to sustainable chemical production using synthesis gas.

公开(公告)号：US20230383317A1

公开(公告)日：2023-11-30

申请号：US18249379

申请日：2020-11-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hyunju OH ,  Youngsoon UM ,  Sun Mi LEE ,  Gyeongtaek GONG ,  Ja Kyong KO

IPC: C12P7/16 ,  C12N1/20

CPC classification number: C12P7/16 ,  C12N1/20

Abstract: The present invention relates to a composition for producing 2,3-butanediol by using ethanol and synthetic gas and a 2,3-butanediol production method. A composition according to an aspect of the present invention includes ethanol as an active ingredient. In the case where a 2,3-butanediol production strain is inoculated into a medium containing the composition and cultured and a synthetic gas is added thereto, 2,3-butanediol, which is a biofuel and a chemical substance, can be economically produced with ethanol serving as a substrate. In the metabolism process, the carbon flux can be concentrated on the production route of the target material, whereby the composition has an excellent effect of increasing the production efficiency of 2,3-butanediol and enhancing the productivity of 2,3-butanediol by controlling only fermentation conditions such as amounts of the synthetic gas or ethanol and stirring speeds of the medium.

公开(公告)号：US20230093685A1

公开(公告)日：2023-03-23

申请号：US17849571

申请日：2022-06-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hyun Ju OH ,  Youngsoon UM ,  Sun Mi LEE ,  Gyeongtaek GONG ,  Ja Kyong KO

IPC: C12P7/06 ,  C12P7/16

Abstract: The present disclosure relates to a composition for preparing hexanol or butanol and a method for preparing hexanol or butanol using ethanol and synthesis gas, wherein the composition according to an aspect of the present disclosure is a medium composition containing ethanol as an active ingredient, and by culturing a strain producing hexanol or butanol after inoculating with a medium containing the composition and supplying synthesis gas, hexanol or butanol can be prepared economically using inexpensive synthesis gas, and hexanol or butanol can be prepared with high efficiency by focusing the flow of a carbon source consumed in a fermentation process to the production of hexanol or butanol.