公开(公告)号：US11773426B2

公开(公告)日：2023-10-03

申请号：US16514814

申请日：2019-07-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Joun ,  Chieh-Yuan Li ,  Brian Reed ,  Craig Obergfell ,  Devin Dressman ,  Abraham Rosenbaum ,  Scott Benson ,  Andi Broka ,  Srinka Ghosh

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6844

CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q1/6844 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537

Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

公开(公告)号：US09150917B2

公开(公告)日：2015-10-06

申请号：US14057450

申请日：2013-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Jessica Lynn Reed ,  Jason Gioia ,  Devin Dressman ,  Nicholas Hapshe ,  Brian Reed ,  John Andrew Sheridan

IPC: C12Q1/68 ,  B01L3/00 ,  G01N33/50 ,  G01N33/543

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0893 ,  C12Q1/6837 ,  G01N33/50 ,  G01N33/543

Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.

Abstract translation: 提供了用于将样品沉积到表面上的方法，组合物，系统，装置和试剂盒。 样品可以包括一个或多个颗粒，并且该表面可以包括一个或多个反应室。 在一些实施方案中，沉积可以包括使用伴随颗粒与样品颗粒的组合。

公开(公告)号：US20180371541A1

公开(公告)日：2018-12-27

申请号：US16123823

申请日：2018-09-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

公开(公告)号：US09587269B2

公开(公告)日：2017-03-07

申请号：US14832772

申请日：2015-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

Abstract translation: 在一些实施方案中，从与支持物连接的核酸模板获得序列信息的方法包括将第一引物与连接于载体的模板链杂交，对核酸模板的一部分进行测序，从而形成延伸的第一引物产物， 与一部分核酸模板互补。在一些实施方案中，该方法还包括将切口引入与延伸的第一引物产物杂交的模板链的一部分中，使用下述方法从模板中降解模板链的一部分： 其中一部分延伸的第一引物保留与模板链的未降解部分杂交，并通过合成测序延伸的第一引物的至少一些单链部分。

公开(公告)号：US10508306B2

公开(公告)日：2019-12-17

申请号：US16123823

申请日：2018-09-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

公开(公告)号：US10081837B2

公开(公告)日：2018-09-25

申请号：US15730536

申请日：2017-10-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

公开(公告)号：US09822411B2

公开(公告)日：2017-11-21

申请号：US15423744

申请日：2017-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.