公开(公告)号：US20170369431A1

公开(公告)日：2017-12-28

申请号：US15529437

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  G01N33/58 ,  C07D311/84 ,  C07C309/51 ,  C07C2603/50

CPC classification number: C07C309/47 ,  C07C309/51 ,  C07C2603/50 ,  C07D311/84 ,  G01N33/582 ,  G01N2400/10

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20180031545A1

公开(公告)日：2018-02-01

申请号：US15532271

申请日：2015-12-03

Applicant: Life Technologies Corporation

Inventor： James STRAY ,  Shaheer KHAN

IPC: G01N33/53 ,  G01N27/447 ,  G01N33/58 ,  G06F19/00 ,  C12Q1/40 ,  C12Q1/68 ,  G01N33/533

CPC classification number: G01N33/5308 ,  C12Q1/40 ,  C12Q1/6876 ,  G01N27/447 ,  G01N27/44726 ,  G01N30/00 ,  G01N30/34 ,  G01N33/533 ,  G01N33/58 ,  G01N33/582 ,  G01N33/66 ,  G01N2030/8813 ,  G01N2030/8836 ,  G01N2333/924 ,  G01N2400/00 ,  G01N2400/22 ,  G01N2458/10 ,  G01N2800/26 ,  G06F19/70 ,  G06F19/709

Abstract: Methods, systems, compositions and kits are described for detecting cleaved glycans from a glycoconjugate. After labeling the glycan with a nucleic acid charged oligomer described herein, the labeled glycans can be separated under the influence of an electric field or based on their detectable tag and identified.

公开(公告)号：US20180222855A1

公开(公告)日：2018-08-09

申请号：US15894057

申请日：2018-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  C07D311/84 ,  C07C309/51 ,  G01N33/58

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20160348092A1

公开(公告)日：2016-12-01

申请号：US15235327

申请日：2016-08-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10

CPC classification number: C12N15/1003 ,  C12N1/06 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

Abstract translation: 本教导涉及从固体材料中提取核酸。 提供了用于从固体生物样品获得核酸的有用的组合物，方法和试剂盒或具有粘附或嵌入粘合剂基底内的生物材料的粘合剂材料。 提取的核酸可用于下游应用，例如生物材料来源的基因分型，检测，定量和鉴定。

公开(公告)号：US20160341640A1

公开(公告)日：2016-11-24

申请号：US15157011

申请日：2016-05-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maxim BREVNOV ,  James NURSE ,  James STRAY

IPC: G01N1/40

CPC classification number: G01N1/4005 ,  G01N2001/4016

Abstract: A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.

Abstract translation: 用于样品处理的装置可以包括具有出口的至少一个室，所述室被配置为接收用于处理的样品，过滤器，所述至少一个室中的至少一些样品部分通过所述过滤器和设置在所述室中的阻挡构件 第一状态以在至少一个室中容纳样品。 在足够的条件下，阻挡构件可以改变到第二状态，以允许包含在腔室中的至少一些样品部分沿流动方向流向出口并通过过滤器。

公开(公告)号：US20240200053A1

公开(公告)日：2024-06-20

申请号：US18379480

申请日：2023-10-12

Applicant: Life Technologies Corporation

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10 ,  C12N1/06 ,  C12Q1/6806

CPC classification number: C12N15/1003 ,  C12N1/06 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

公开(公告)号：US20210198655A1

公开(公告)日：2021-07-01

申请号：US17146163

申请日：2021-01-11

Applicant: Life Technologies Corporation

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10 ,  C12N1/06 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

公开(公告)号：US20180371449A1

公开(公告)日：2018-12-27

申请号：US16013938

申请日：2018-06-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10 ,  C12Q1/6806 ,  C12N1/06

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.