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    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS
    4.
    发明申请
    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS 有权
    序列合成方法中的替代核酸流

    公开(公告)号：US20130172201A1

    公开(公告)日：2013-07-04

    申请号：US13689252

    申请日：2012-11-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  John DAVIDSON

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

    Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

    Abstract translation: 通过使用按顺序合成技术测序多核苷酸链的方法。 为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。 与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。 这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES
    6.
    发明申请
    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES 审中-公开

    公开(公告)号：US20200017846A1

    公开(公告)日：2020-01-16

    申请号：US16542121

    申请日：2019-08-15

    Applicant: Life Technologies Corporation

    Inventor： John DAVIDSON ,  Theo NIKIFOROV ,  Guobin LUO

    IPC: C12N11/10 ,  C12N9/12

    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES
    7.
    发明申请
    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES 审中-公开

    公开(公告)号：US20180105805A1

    公开(公告)日：2018-04-19

    申请号：US15786223

    申请日：2017-10-17

    Applicant: Life Technologies Corporation

    Inventor： John DAVIDSON ,  Theo Nikiforov ,  Guobin Luo

    IPC: C12N11/10 ,  C12N9/12

    CPC classification number: C12N11/10 ,  C12N9/1241 ,  C12Q1/68 ,  G01N2333/9126

    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME
    9.
    发明申请
    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 有权
    改良蛋白质及其制备方法和使用方法

    公开(公告)号：US20150260680A1

    公开(公告)日：2015-09-17

    申请号：US14724354

    申请日：2015-05-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John DAVIDSON ,  Wolfgang HINZ ,  Jonathan ROTHBERG ,  Richard WHITAKER

    IPC: G01N27/414

    CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

    Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的经修饰的核酸结合蛋白质的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这些修饰蛋白质的方法，例如基于离子的核酸测序反应。

    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS
    10.
    发明申请
    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS 审中-公开
    序列合成方法中的替代核酸流

    公开(公告)号：US20140031238A1

    公开(公告)日：2014-01-30

    申请号：US13859667

    申请日：2013-04-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  John DAVIDSON

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

    Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

    Abstract translation: 通过使用按顺序合成技术测序多核苷酸链的方法。 为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。 与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。 这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

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