公开(公告)号：US20230085832A1

公开(公告)日：2023-03-23

申请号：US17936947

申请日：2022-09-30

Applicant: Life Technologies Corporation

Inventor： Kyle GEE ,  Kathleen KIHN ,  Yi-Zhen HU ,  Yexin WU ,  Kathleen FREE

IPC: G01N21/64 ,  C07D311/82 ,  C07D209/10 ,  C07F15/00 ,  C07F9/24

Abstract: Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

公开(公告)号：US20170166943A1

公开(公告)日：2017-06-15

申请号：US15385340

申请日：2016-12-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian AGNEW ,  Kyle GEE ,  Schuyler CORRY

IPC: C12P21/00 ,  C07K14/47 ,  G01N33/533 ,  C07K16/00

Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.

公开(公告)号：US20150355186A1

公开(公告)日：2015-12-10

申请号：US14829346

申请日：2015-08-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Evan ANTOULINAKIS ,  Kyle GEE ,  Aleksey RUKAVISHNIKOV

IPC: G01N33/58 ,  C07D311/82 ,  C07D311/18 ,  C07D493/14 ,  C07D495/22 ,  C07F5/02 ,  C07D209/18

CPC classification number: G01N33/58 ,  C07D209/14 ,  C07D209/18 ,  C07D311/18 ,  C07D311/82 ,  C07D401/10 ,  C07D493/14 ,  C07D495/22 ,  C07F5/022 ,  C09B11/24 ,  C09B11/26 ,  C09B23/04 ,  C09B23/08 ,  C09B45/00 ,  C09B56/16 ,  G01N33/532 ,  G01N33/582

Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.

Abstract translation: 本公开涉及能够将报告分子与载体分子或固体支持物缀合的反应性酯试剂。 反应性酯剂具有以下通式：其中在整个申请中描述了变量。

公开(公告)号：US20150219634A1

公开(公告)日：2015-08-06

申请号：US14615818

申请日：2015-02-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Michael Janes ,  Iain Johnson ,  Hee Chol Kang

IPC: G01N33/52 ,  C07D491/06

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

Abstract translation: 描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。 该化合物可用于检测活细胞中氧化应激和相关过程。

公开(公告)号：US20150104823A1

公开(公告)日：2015-04-16

申请号：US14521346

申请日：2014-10-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel BEACHAM ,  Kyle GEE

IPC: G01N33/84 ,  G01N33/68 ,  C12Q1/02

CPC classification number: G01N33/84 ,  C12Q1/02 ,  G01N33/6872 ,  G01N2333/705

Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.

Abstract translation: 本发明涉及用于检测细胞中离子通道的活性的方法。 所述方法包括向具有离子通道的电池提供加载缓冲溶液。 加载缓冲液包含至少一种铊指示剂（例如，环境敏感的发光染料）和氯离子的生理浓度。 所述方法还包括向细胞提供刺激缓冲液，其中刺激缓冲液包含铊（例如铊离子）。 提供刺激缓冲液使铊通过离子通道流入细胞。 在提供刺激缓冲液之后，检测细胞中染料的发光（例如荧光）。 在存在或不存在铊的情况下，染料的发光可以改变。 该方法可用于测量铊通过离子通道的流入或流出。

公开(公告)号：US20140220612A1

公开(公告)日：2014-08-07

申请号：US14247619

申请日：2014-04-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert BATCHELOR ,  Yue GE ,  Iain JOHNSON ,  Wai-Yee LEUNG ,  Jixiang LIU ,  Brian PATCH ,  Peter SMALLEY ,  Thomas STEINBERG ,  Kyle GEE

IPC: C12Q1/28 ,  C09B19/00

CPC classification number: C09B19/00 ,  C07D265/38 ,  C07D413/12 ,  C07D495/04 ,  C12Q1/28

Abstract: The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.

Abstract translation: 本发明提供可用于各种测定形式的新型氟化聚砜化合物。 还提供了使用包括本发明化合物的化合物和试剂盒的方法以及详细说明化合物在一种或多种测定形式中的用途的说明书。

公开(公告)号：US20200261602A1

公开(公告)日：2020-08-20

申请号：US16816015

申请日：2020-03-11

Applicant: Life Technologies Corporation ,  Pierce Biotechnology, Inc.

Inventor： Surbhi DESAI ,  Marie Christine NLEND ,  Kyle GEE ,  Matthew BAKER ,  Robert AGGELER ,  Scott SWEENEY ,  Aleksey RUKAVISHNIKOV ,  Shih-Jung HUANG

IPC: A61K49/00 ,  G01N33/58 ,  G01N33/68 ,  G01N33/533 ,  A61K47/18 ,  A61K47/10

Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.

公开(公告)号：US20200216871A1

公开(公告)日：2020-07-09

申请号：US16738274

申请日：2020-01-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian AGNEW ,  Kyle GEE ,  Tamara NYBERG

IPC: C12P21/00 ,  A61K47/54 ,  G01N33/531 ,  C07K1/13 ,  G01N33/532 ,  G01N33/50 ,  G01N33/533 ,  C07K16/00 ,  G01N33/58 ,  C07K14/47

Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.

公开(公告)号：US20180078662A1

公开(公告)日：2018-03-22

申请号：US15826593

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian AGNEW ,  Robert AGGELER ,  Hee Chol KANG ,  Aimei CHEN ,  Kyle GEE

IPC: A61K51/10 ,  G01N33/534 ,  C12P21/00 ,  C07K16/18 ,  G01N33/68 ,  C12P21/06 ,  C07K14/435 ,  G01N33/58

CPC classification number: A61K51/10 ,  C07K14/435 ,  C07K16/18 ,  C12P21/005 ,  C12P21/06 ,  G01N33/534 ,  G01N33/582 ,  G01N33/6854

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

公开(公告)号：US20170362624A1

公开(公告)日：2017-12-21

申请号：US15474901

申请日：2017-03-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian AGNEW ,  Kyle GEE ,  Tamara NYBERG

IPC: C12P21/00 ,  A61K47/54 ,  C07K14/47 ,  G01N33/50 ,  G01N33/533 ,  G01N33/532 ,  G01N33/531 ,  G01N33/58 ,  C07K16/00 ,  G01N33/534

CPC classification number: C12P21/005 ,  A61K47/549 ,  C07K1/13 ,  C07K14/47 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C12Y302/01096 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/531 ,  G01N33/532 ,  G01N33/533 ,  G01N33/534 ,  G01N33/581 ,  G01N33/582 ,  G01N33/583 ,  Y10T436/17

Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.