公开(公告)号：US20150105275A1

公开(公告)日：2015-04-16

申请号：US14233711

申请日：2013-11-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Wong ,  Caifu Chen ,  Yalei Wu ,  Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/68

CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

Abstract translation: 本文提供了用于捕获，检测和定量成熟小RNA的方法，组合物和试剂盒。 方法的实施方案包括在5'和3'半简并连接夹板的存在下分别将5'和3'连接衔接子连接到成熟小RNA的5'和3'末端以产生连接产物。 其他实施方案包括具有通用逆转录引物的逆转录聚腺苷酸化成熟小RNA，并且在半退化连接夹板存在下将衔接子连接到cDNA的3'末端以产生cDNA连接产物。

公开(公告)号：US20180208979A1

公开(公告)日：2018-07-26

申请号：US15301947

申请日：2015-04-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yalei Wu ,  David Keys

IPC: C12Q1/6851 ,  G06F19/12 ,  G06F19/22 ,  G06F19/24 ,  G06F19/26

CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/16 ,  C12Q2545/114 ,  G16B5/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  G16B45/00

Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

公开(公告)号：US11208688B2

公开(公告)日：2021-12-28

申请号：US15887159

申请日：2018-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Wong ,  Caifu Chen ,  Yalei Wu ,  Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6848

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

公开(公告)号：US20170177791A1

公开(公告)日：2017-06-22

申请号：US15301949

申请日：2015-04-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yalei Wu ,  David Keys

IPC: G06F19/24 ,  G06F19/22 ,  G06F19/26 ,  G06F19/12

CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/16 ,  C12Q2545/114 ,  G16B5/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  G16B45/00

Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

公开(公告)号：US10385386B2

公开(公告)日：2019-08-20

申请号：US15301947

申请日：2015-04-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yalei Wu ,  David Keys

IPC: G16B5/00 ,  C12Q1/6851 ,  G16B30/00 ,  G16B40/00 ,  G16B45/00 ,  G16B25/00 ,  C12Q1/686

Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

公开(公告)号：US09920360B2

公开(公告)日：2018-03-20

申请号：US14233711

申请日：2013-11-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Wong ,  Caifu Chen ,  Yalei Wu ,  Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.