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公开(公告)号：US11857643B2
公开(公告)日：2024-01-02
申请号：US16816015
申请日：2020-03-11
发明人： Surbhi Desai , Marie Christine Nlend , Kyle Gee , Matthew Baker , Robert Aggeler , Scott Sweeney , Aleksey Rukavishnikov , Shih-Jung Huang
CPC分类号： A61K49/0015 , A61K47/10 , A61K47/18 , A61K49/0058 , G01N33/533 , G01N33/582 , G01N33/68
摘要： This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.
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公开(公告)号：US11493446B2
公开(公告)日：2022-11-08
申请号：US16205702
申请日：2018-11-30
发明人： Kyle Gee , Kathleen Kihn , Yi-Zhen Hu , Yexin Wu , Kathleen Free
IPC分类号： G01N21/64 , C07D311/82 , C07D209/10 , C07F15/00 , C07F9/24
摘要： Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.
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公开(公告)号：US11193057B2
公开(公告)日：2021-12-07
申请号：US16324730
申请日：2017-08-24
发明人： Adam York , Eric Welch , Kyle Gee , Xin Wang , Robert Aggeler , Yi-Zhen Hu
IPC分类号： C09K11/02 , C09K11/06 , G01N33/50 , G01N33/58 , H04L12/18 , C09B67/00 , G01N33/533 , H04L29/08
摘要： Water-soluble, fluorescent particles and compositions, kits, and methods of making and using such particles are disclosed. Processes for preparing fluorescent particles and for controlling the size, polydispersity and optical properties of such particles also are provided.
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公开(公告)号：US20200333351A1
公开(公告)日：2020-10-22
申请号：US16838004
申请日：2020-04-01
发明人： Kyle Gee , Upinder Singh , Scott Grecian , Scott Clarke
IPC分类号： G01N33/58 , G01N33/533 , C07D213/74 , C07D213/79 , C07D213/81 , C07D215/12 , C07D401/04 , C07D235/14 , C07D213/53 , C07D213/68 , C07D213/78 , C09B23/06 , C09B23/08 , C09B11/24 , C07D401/14 , C07K14/575
摘要： Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.
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公开(公告)号：US10739330B2
公开(公告)日：2020-08-11
申请号：US16002991
申请日：2018-06-07
发明人： Kyle Gee , Hee Chol Kang , Wenjun Zhou , Bradley Dubbels , Michael Olszowy , Michael O'Grady
IPC分类号： G01N33/50 , C09B11/28 , C07K16/28 , C07C309/61 , C07D471/04
摘要： Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.
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公开(公告)号：US10006918B2
公开(公告)日：2018-06-26
申请号：US15176872
申请日：2016-06-08
发明人： Aimei Chen , Kyle Gee , Hee Chol Kang
IPC分类号： C07F5/02 , C09B11/24 , G01N21/64 , G01N33/50 , G01N33/64 , C07D311/82 , C07D471/06 , C07F9/54 , C07F9/655 , G01N33/52 , C09K11/06
CPC分类号： G01N33/64 , C07D311/82 , C07D471/06 , C07F5/022 , C07F9/5442 , C07F9/65522 , C09B11/24 , C09K11/06 , C09K2211/10 , C09K2211/1007 , C09K2211/1014 , C09K2211/1022 , C09K2211/1033 , C09K2211/1055 , C09K2211/1088 , G01N21/6428 , G01N21/6486 , G01N33/50 , G01N33/52 , G01N2021/6439 , Y10T436/143333
摘要： The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.
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7.发明授权Modified nucleic acid binding cyanine dyes for the detection of reactive oxygen species 有权用于检测活性氧的修饰的核酸结合花青染料公开(公告)号：US09518981B2
公开(公告)日：2016-12-13
申请号：US14384843
申请日：2013-02-26
发明人： Yi-Zhen Hu , Aimei Chen , Hee Chol Kang , Kyle Gee , Bhaskar Mandavilli
IPC分类号： C07D215/00 , G01N33/52 , C09B23/02 , C09B23/04 , C09B23/06 , G01N33/84 , C07D417/06
摘要： Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.
摘要翻译： 本文公开了通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原性核酸结合花青染料，其染料是用于检测ROS的探针，并测定体外和/或体内细胞中的氧化应激。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。
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公开(公告)号：US09388420B2
公开(公告)日：2016-07-12
申请号：US14600155
申请日：2015-01-20
IPC分类号： A01N43/04 , A61K31/70 , C12N15/67 , A61K31/7088 , A61K31/7084 , A61K31/7115 , A61K31/712 , C07C247/04 , C07D213/82 , C07H19/207 , C07H21/02 , C12Q1/68 , C12N15/11 , A61K48/00
CPC分类号： C12N15/67 , A61K31/7084 , A61K31/7088 , A61K31/7115 , A61K31/712 , A61K39/12 , A61K39/39 , A61K48/00 , A61K2039/53 , A61K2039/55511 , C07C247/04 , C07C323/60 , C07D213/82 , C07H1/00 , C07H1/02 , C07H1/04 , C07H19/207 , C07H21/02 , C12N7/00 , C12N15/11 , C12N2770/36134 , C12N2770/36171 , C12P19/34 , C12Q1/68
摘要： Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.
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公开(公告)号：US20150252373A1
公开(公告)日：2015-09-10
申请号：US14600155
申请日：2015-01-20
IPC分类号： C12N15/67 , C07D213/82 , C12N15/11 , C07C247/04
CPC分类号： C12N15/67 , A61K31/7084 , A61K31/7088 , A61K31/7115 , A61K31/712 , A61K39/12 , A61K39/39 , A61K48/00 , A61K2039/53 , A61K2039/55511 , C07C247/04 , C07C323/60 , C07D213/82 , C07H1/00 , C07H1/02 , C07H1/04 , C07H19/207 , C07H21/02 , C12N7/00 , C12N15/11 , C12N2770/36134 , C12N2770/36171 , C12P19/34 , C12Q1/68
摘要： Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.
摘要翻译： 提供炔基衍生的帽类似物，炔基修饰的封端RNA，1,4-二取代的三唑衍生的封端RNA，制备方法，分离方法及其用途。 “点击”修改有助于检测和分离封闭的RNA，通过“点击”反应形成的1,4-二取代的三唑衍生物可用于产生RNA转录物和编码的蛋白质。
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公开(公告)号：US20150218379A1
公开(公告)日：2015-08-06
申请号：US14402358
申请日：2013-03-14
发明人： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou
CPC分类号： C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
摘要： Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
摘要翻译： 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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