公开(公告)号：US20250137002A1

公开(公告)日：2025-05-01

申请号：US18983993

申请日：2024-12-17

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  A01H6/20 ,  A01H6/46 ,  A01H6/54 ,  A01H6/60 ,  C12N1/20 ,  C12N5/00 ,  C12N5/04 ,  C12N9/10 ,  C12R1/41 ,  G01N33/00

Abstract: The present invention relates to methods and compositions for transforming soybean, corn. cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US12163196B2

公开(公告)日：2024-12-10

申请号：US17319657

申请日：2021-05-13

Applicant: Monsanto Technology LLC

Inventor： David Butruille ,  Kevin L. Deppermann ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Michael W. Petersen ,  Bruce Schnicker ,  John Tamulonis

IPC: C12Q1/6895 ,  A01H1/04 ,  A01H5/10 ,  A01H6/54 ,  C12N15/82

Abstract: A method is provided for use in analyzing seeds, in a population of seeds, for one or more desired characteristic. The method includes removing tissue from individual seeds in a population of seeds using an automated seed sampler, while preserving germination viability of the seeds, and analyzing the tissue for the presence or absence of one or more desired characteristic. The method then includes, based on the analysis of the tissue, quantifying the one or more desired characteristic for each of the individual seeds that possess the one or more desired characteristic.

公开(公告)号：US11851667B2

公开(公告)日：2023-12-26

申请号：US17175479

申请日：2021-02-12

Applicant: Monsanto Technology LLC

Inventor： Jaishree M. Chittoor ,  Stanislaw Flasinski ,  Mohammed Oufattole ,  Michael W. Petersen

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8216 ,  C07K14/415 ,  C12N15/8222

Abstract: The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

公开(公告)号：US11629357B2

公开(公告)日：2023-04-18

申请号：US17079348

申请日：2020-10-23

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Susan J. Johnson ,  Michael W. Petersen ,  David Walters ,  Paul S. Chomet

IPC: C12N15/82 ,  C12N9/10

Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

公开(公告)号：US11530963B2

公开(公告)日：2022-12-20

申请号：US16993178

申请日：2020-08-13

Applicant: Monsanto Technology LLC

Inventor： Kevin L. Deppermann ,  Michael W. Petersen

IPC: A01C1/00 ,  G01N1/04 ,  A01C1/02 ,  A01H1/02 ,  A01H1/04 ,  A01H4/00 ,  C12Q1/6895 ,  G01N35/00 ,  G01N35/04

Abstract: An automated method for sampling seeds generally includes removing material from a seed at an automated sampling station and then, after removing the material from the seed, detecting, by at least one sensor, movement of the seed out of the automated sampling station. The automated method may also include, after removing the material from the seed, transferring the seed from the automated sampling station to a seed container and transferring the material removed from the seed from the automated sampling station to a sample container.

公开(公告)号：US10705102B2

公开(公告)日：2020-07-07

申请号：US14685047

申请日：2015-04-13

Applicant: Monsanto Technology LLC

Inventor： Kevin L. Deppermann ,  Michael W. Petersen ,  Allen N. Ondes ,  David W. Finley ,  William M. Fischer ,  John M. Jensen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather M. Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC: A01C1/00 ,  G01N35/00 ,  G01N33/00 ,  G01N33/50 ,  G01N1/20 ,  G01N1/28 ,  A01C1/02 ,  G01N1/04 ,  G01N1/00

Abstract: A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

公开(公告)号：US09938535B2

公开(公告)日：2018-04-10

申请号：US14949694

申请日：2015-11-23

Applicant: Monsanto Technology LLC

Inventor： Jaishree M. Chittoor ,  Amy J. Miyamoto ,  Amy M. Nichols ,  Mohammed Oufattole ,  Michael W. Petersen

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8241 ,  C12N15/8261 ,  C12N15/8274 ,  C12N15/8279

Abstract: Recombinant DNA molecules and constructs useful for modulating gene expression in plants, including molecules derived from Medicago truncatula sequences, are provided. Transgenic plants, plant cells, plant parts, and seeds comprising recombinant DNA molecules operably linked to heterologous transcribable DNA molecules are further provided, as are methods of their use.

公开(公告)号：US20150020241A1

公开(公告)日：2015-01-15

申请号：US14298785

申请日：2014-06-06

Applicant: Monsanto Technology LLC

Inventor： Wei Wu ,  Qi Wang ,  Michael Edgerton ,  Kim Beazley ,  Jill Deikman ,  Michael W. Petersen ,  Bei Zhang

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  A01H5/00 ,  C12N9/1085 ,  C12N15/8289 ,  C12Y205/01006

Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from the S-adenosyl methionine synthetase (SAMS3) gene of Arabidopsis thaliana and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Arabidopsis thaliana regulatory polynucleotide sequences, and methods for preparing and using the same.

Abstract translation: 本发明提供从拟南芥的S-腺苷甲硫氨酸合成酶（SAMS3）基因中分离的非编码调控元件多核苷酸分子，并且可用于在植物中表达转基因。 本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有拟南芥调节多核苷酸序列的种子，以及制备和使用拟南芥调节多核苷酸序列的方法。

公开(公告)号：US08872000B2

公开(公告)日：2014-10-28

申请号：US13899452

申请日：2013-05-21

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N15/62 ,  A01H1/04 ,  G01N33/00 ,  C12N5/04 ,  A01H4/00

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

Abstract translation: 本发明涉及使用壮观霉素作为转化外植体的选择剂转化大豆，玉米，棉花或油菜外植体的方法和组合物。 该方法还可以包括在转化和再生过程中用细胞分裂素处理外植体。

公开(公告)号：US12195736B2

公开(公告)日：2025-01-14

申请号：US18481464

申请日：2023-10-05

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  C12N5/00 ,  C12N5/04 ,  C12N9/10 ,  G01N33/00 ,  A01H6/20 ,  A01H6/46 ,  A01H6/54 ,  A01H6/60 ,  C12N1/20 ,  C12R1/41

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.